Laminin-alpha6beta1 integrin interaction enhances survival and proliferation and modulates steroidogenesis of ovine granulosa cells

Bellego, Frédérique Le; Pisselet, C.; Huet, Clotilde; Monget, Philippe; Monniaux, Danielle

doi:10.1677/joe.0.1720045

Cited by 54 publications

(51 citation statements)

References 55 publications

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“…Integrin a6/b1 is the receptor for laminin on GCs and it has been shown that the interaction between them in GCs enhances survival and proliferation and modulates steroidogenesis (Le Bellego et al, 2002). Whether the ITGA6 antisense lncRNA directly regulates the expression of its ITGA6 host gene requires further investigation.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the human cumulus cell transcriptome during final follicular maturation and ovulation

Yerushalmi

Salmon‐Divon

Yung

et al. 2014

MHR: Basic Science of Reproductive Medicine

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Cumulus expansion and oocyte maturation are central processes in ovulation. Knowledge gained from rodent and other mammalian models has revealed some of the molecular pathways associated with these processes. However, the equivalent pathways in humans have not been thoroughly studied and remain unidentified. Compact cumulus cells (CCs) from germinal vesicle cumulus oocyte complexes (COCs) were obtained from patients undergoing in vitro maturation (IVM) procedures. Expanded CCs from metaphase 2 COC were obtained from patients undergoing IVF/ICSI. Global transcriptome profiles of the samples were obtained using state-of-the-art RNA sequencing techniques. We identified 1746 differentially expressed (DE) genes between compact and expanded CCs. Most of these genes were involved in cellular growth and proliferation, cellular movement, cell cycle, cell-to-cell signaling and interaction, extracellular matrix and steroidogenesis. Out of the DE genes, we found 89 long noncoding RNAs, of which 12 are encoded within introns of genes known to be involved in granulosa cell processes. This suggests that unique noncoding RNA transcripts may contribute to the regulation of cumulus expansion and oocyte maturation. Using global transcriptome sequencing, we were able to generate a library of genes regulated during cumulus expansion and oocyte maturation processes. Analysis of these genes allowed us to identify important new genes and noncoding RNAs potentially involved in COC maturation and cumulus expansion. These results may increase our understanding of the process of oocyte maturation and could ultimately improve the efficacy of IVM treatment.

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Section: Discussionmentioning

confidence: 99%

Characterization of the human cumulus cell transcriptome during final follicular maturation and ovulation

Yerushalmi

Salmon‐Divon

Yung

et al. 2014

MHR: Basic Science of Reproductive Medicine

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“…With continued in vitro culture, the expression of many parts of these genes is enhanced in the AF group. In addition, ITGA6, THBS1, THBS2, VEGF, AMH, FST and INHBA were reported to express high in growing follicles (Le Bellego et al 2002, Nilsson et al 2003, Greenaway et al 2005, Tesfaye et al 2009, Rosales-Torres et al 2010, Bonnet et al 2011 and Table 5 Expression of marker genes in granulosa cells.…”

Section: Discussionmentioning

confidence: 99%

Estradiol supports in vitro development of bovine early antral follicles

Endo¹,

Kawahara-Miki²,

Cao³

et al. 2013

Reproduction

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Antrum formation and estradiol (E 2 ) secretion are specific features of oocyte and granulosa cell complexes (OGCs). This study investigates the effect of E 2 on the in vitro development of bovine OGCs derived from early antral follicles as well as on the expression of genes in granulosa cells (GCs). The supplementation of culture medium with either E 2 or androstenedione (A 4 ) improved the in vitro development of OGCs and the nuclear maturation of enclosed oocytes. When OGCs were cultured in medium containing A 4 , developmentally competent OGCs secreted more E 2 than OGCs that were not competent. In addition, fulvestrant inhibited the effect of both E 2 and A 4 on OGCs development. Comprehensive gene expression analysis using next-generation sequence technology was conducted for the following three types of GCs: i) GCs of OGCs cultured for 4 days with E 2 (1 mg/ml; E 2 (C)), ii) GCs of OGCs cultured for 4 days without E 2 (E 2 (K)) or iii) OGCs that formed clear antrum after 8 days of in vitro culture in medium containing E 2 (1 mg/ml; AF group). GCs of the E 2 (C) group had a similar gene expression profile to the profile reported previously for the in vivo development of large follicles. This genetic profile included factors implicated in the up-regulation of E 2 biosynthesis and down-regulation of cytoskeleton and extracellular matrices. In addition, a novel gene expression profile was found in the AF group. In conclusion, E 2 impacts the gene expression profile of GCs to support the in vitro development of OGCs.Reproduction (2013) 145 85-96

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“…Then, the follicles were classified according to their size: 1-3, 3-5, 5-8, and O8 mm. Follicular fluids and granulosa cells were recovered from the follicles of both ovaries in each goat, as described previously (Le Bellego et al 2002). For each goat, the follicular fluids were pooled according to the size of the follicles (one to three pools of follicular fluids per follicular size class, when follicles of the size class were present) and stored at K20 8C until the AMH assay was performed.…”

Section: Plasma Follicular Fluid and Granulosa Cell Collectionmentioning

confidence: 99%

Anti-Müllerian hormone as a predictive endocrine marker for embryo production in the goat

Monniaux¹,

Baril²,

Lainé³

et al. 2011

Reproduction

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Recently, we demonstrated the relationship between anti-Mü llerian hormone (AMH) circulating concentrations, ovarian follicles, and embryo production in cattle. However, they have not yet been established in a species with a seasonal breeding activity. Thus, goats were subjected to repeated in vivo embryo production during the breeding season, at the end of the breeding season, and at the end of the anestrus season. Embryo production after FSH treatment was highly repeatable for each goat. Plasma AMH concentrations, measured before the first FSH treatment, were highly correlated with the number of collected, transferable, and freezable embryos, resulting from the three sessions of embryo production. Plasma AMH concentrations transiently decreased after each exogenous FSH treatment, but they showed little change with season, and no relationship was observed between AMH and endogenous FSH concentrations during seasonal transitions. Follicles of 1-5 mm in diameter were the main target of the FSH treatment and were major contributors to circulating AMH concentrations. Granulosa cell AMH expression decreased as the follicle approached terminal development, while the expression of maturation markers (CYP19A1 and FSHR) increased. In conclusion, circulating AMH concentrations can be predictive of the capacity of a donor goat to produce high or low numbers of high-quality embryos. This prediction could be accurately made from a single blood measurement of AMH during either breeding or anestrus seasons. Variability in the number of gonadotropin-responsive follicles of 1-5 mm in diameter between individuals resulted in the differences in circulating AMH concentrations measured between individuals.

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Laminin-alpha6beta1 integrin interaction enhances survival and proliferation and modulates steroidogenesis of ovine granulosa cells

Cited by 54 publications

References 55 publications

Characterization of the human cumulus cell transcriptome during final follicular maturation and ovulation

Characterization of the human cumulus cell transcriptome during final follicular maturation and ovulation

Estradiol supports in vitro development of bovine early antral follicles

Anti-Müllerian hormone as a predictive endocrine marker for embryo production in the goat

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