Lampropedia cohaerens sp. nov., a biofilm-forming bacterium isolated from microbial mats of a hot water spring, and emended description of the genus Lampropedia

Abstract

A biofilm-forming, Gram-stain-negative, aerobic, catalase-positive but oxidase-negative strain, designated CT6 T , was isolated from the microbial mats (,45 8C) of a hot water spring, located within the Himalayan ranges at Manikaran, Himachal Pradesh, India. Strain CT6 T formed white, smooth colonies with irregular margins. Transmission electron microscopy revealed coccoid, non-flagellated cells with wavy boundaries. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CT6 T belongs to the genus Lampropedia with a sequence similarity value of 95.4 % to the sole member of this genus, Lampropedia hyalina ATCC 11041 T . Strain CT6 T was found to have phosphatidylethanolamine and phosphatidylglycerol as the major polar lipids. The major cellular fatty acids were C 16 : 0 , summed feature 8 (C 18 : 1 v7c and/or C 18 : 1 v6c), C 14 : 0 , C 19 : 0 v8c cyclo and summed feature 3 (C 16 : 1 v7c and/or C 16 : 1 v6c). The major respiratory quinone was ubiquinone-8. The major polyamines were putrescine, spermidine and the betaproteobacterial-specific 2-hydroxyputrescine. The DNA G+C content was 63.5 mol%. Based on the genotypic, phenotypic, physiological and biochemical data, strain CT6 T is considered to represent a novel species of the genus Lampropedia, for which the name Lampropedia cohaerens sp. nov. is proposed

Bacterial and Archaeal Viruses of Himalayan Hot Springs at Manikaran Modulate Host Genomes

“…In the past, the sequence-based viral characterization as well as electron and ion microscopy have been used for elucidating viral diversity within geothermally heated ecosystems (Häring et al, 2005). A series of Himalayan hot springs (surface temperature > 95°C) located at an altitude of 1,760 m at Manikaran (32°02′N, 72°21′E) have previously been investigated using sequencing approaches to characterize the bacterial communities (Dwivedi et al, 2012; Mahato et al, 2014; Sharma et al, 2014, 2016b; Tripathi et al, 2016). Culture-independent analysis of the sediment and microbial mat samples demonstrated unexpectedly high microbial diversity in these hot springs (Sangwan et al, 2015; Sharma et al, 2016a).…”

Draft genome sequence of Lampropedia cohaerens strain CT6T isolated from arsenic rich microbial mats of a Himalayan hot water spring

“…This Gram-stain-negative, aerobic bacterial strain, forms white, smooth colonies with irregular margins on LB agar [6]. Transmission electron microscopy (TEM) revealed coccoid, unflagellated cells approximately 0.62 μm × 0.39 μm in dimension (Fig.…”

“…L. hyalina was isolated from activated sludge, and was tested for its phosphate removal capabilities and was classified as belonging to the functional group of polyphosphate accumulating microorganisms [ 5 ]. Another species, L. cohaerens strain CT6 T [ 6 ] was isolated from arsenic rich microbial mats of a Himalayan hot water spring from Manikaran, India as a continuation to our efforts to explore the culturable [ 7 – 10 ] and unculturable [ 11 ] diversity at the Himalayan hot spring to understand the role played by niche specific genetic determinants in shaping the genomes of organisms inhabiting this stressed niche. L. cohaerens , a biofilm forming and arsenic tolerating bacterium [ 6 ], showed limited carbohydrate assimilation potential but could utilize some organic acids.…”

“…Another species, L. cohaerens strain CT6 T [ 6 ] was isolated from arsenic rich microbial mats of a Himalayan hot water spring from Manikaran, India as a continuation to our efforts to explore the culturable [ 7 – 10 ] and unculturable [ 11 ] diversity at the Himalayan hot spring to understand the role played by niche specific genetic determinants in shaping the genomes of organisms inhabiting this stressed niche. L. cohaerens , a biofilm forming and arsenic tolerating bacterium [ 6 ], showed limited carbohydrate assimilation potential but could utilize some organic acids. Currently, the genus Lampropedia is represented by three species, L. hyalina ATCC 11041 T [ 12 ], “ L. puyangensis 2-bin T ” (not validly published) [ 13 ] and L. cohaerens CT6 T [ 6 ], leading to the description of the genus being emended [ 6 ], however, the genomic potential of this small group remains unresolved.…”

“…L. cohaerens , a biofilm forming and arsenic tolerating bacterium [ 6 ], showed limited carbohydrate assimilation potential but could utilize some organic acids. Currently, the genus Lampropedia is represented by three species, L. hyalina ATCC 11041 T [ 12 ], “ L. puyangensis 2-bin T ” (not validly published) [ 13 ] and L. cohaerens CT6 T [ 6 ], leading to the description of the genus being emended [ 6 ], however, the genomic potential of this small group remains unresolved. The genome of strain CT6 T , which is the type strain for Lampropedia cohaerens was sequenced in order to supplement the phenotypic taxonomical observations with genetic data and obtain genomic insights into heavy metal resistance and metabolic potential of gene complements of this microbial mat dweller.…”