LAMTOR3 is a prognostic biomarker in kidney renal clear cell carcinoma

Gong, Yun; Y, Lv; Xu, Fang; Xiu, Youcheng; Lu, Yinhui; Liu, Zan; Deng, Leihong

doi:10.1002/jcla.24648

Cited by 4 publications

(2 citation statements)

References 23 publications

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“…In addition, LAMTOR3 expression was significantly decreased in renal clear cell carcinoma compared with normal renal tissue. LAMTOR3 may be a potential marker for the diagnosis and treatment of renal clear cell carcinoma (Gong et al 2022 ). Song et al ( 2015 ) found that LAMTOR3 polymorphisms may be a potential biomarker of genetic susceptibility to gastric cancer.…”

Section: Discussionmentioning

confidence: 99%

Immunogenic profiling of metastatic uveal melanoma discerns a potential signature related to prognosis

Wang,

Liu,

Sun

et al. 2024

J Cancer Res Clin Oncol

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Background Uveal melanoma (UM) is an aggressive intraocular malignant tumor. The present study aimed to identify the key genes associated with UM metastasis and established a gene signature to analyze the relationship between the signature and prognosis and immune cell infiltration. Later, a predictive model combined with clinical variables was developed and validated. Methods Two UM gene expression profile chip datasets were downloaded from TCGA and GEO databases. Immune-related genes (IRGs) were obtained from IMPORT database. First, these mRNAs were intersected with IRGs, and weighted gene co-expression network analysis (WGCNA) was used to identify the co-expression of genes primarily associated with metastasis of UM. Univariate Cox regression analysis screened the genes related to prognosis. LASSO-Cox established a risk score to distinguish high-risk group and low-risk group. Then the GSEA enrichment pathway and immune cell infiltration of the two groups were compared. And combined with clinical variables, a predictive model was constructed. The time-dependent receiver operating characteristic (ROC) curve, calibration curve, and decision curve analysis (DCA) curve were used to verify the stability and accuracy of the final predictive model, and a nomogram was then drawn. Results The MEblack, MEpurple, and MEblue modules were significantly associated with the metastasis of UM patients (P value < 0.001, = 0.001, = 0.022, respectively). Four genes (UBXN2B, OTUD3, KAT8, LAMTOR2) were obtained by Pearson correlation analysis, weighted gene correlation network analysis (WGCNA), univariate Cox, and LASSO-Cox. And a novel prognostic risk score was established. Immune-related prognostic signature can well classify UM patients into high-risk and low-risk groups. Kaplan–Meier curve showed that the OS of high-risk patients was worse than that of low-risk patients. In addition, the risk score played an important role in evaluating the signaling pathway and immune cell infiltration of UM patients in high-risk and low-risk groups. Both the training set and validation set of the model showed good predictive accuracy in the degree of differentiation and calibration (e.g., 1-year overall survival: AUC = 0.930 (0.857–1.003)). Finally, a nomogram was established to serve in clinical practice. Significance UM key gene signature and prognosis predictive model might provide insights for further investigation of the pathogenesis and development of UM at the molecular level, and provide theoretical basis for determining new prognostic markers of UM and immunotherapy.

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Section: Discussionmentioning

confidence: 99%

Immunogenic profiling of metastatic uveal melanoma discerns a potential signature related to prognosis

Wang,

Liu,

Sun

et al. 2024

J Cancer Res Clin Oncol

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“…Tubulin alpha-I a cell division, cell movement, microtubule based process, cell junction organization, cytoskeleton organization and cytoskeleton dependent intracellular transport upregulated in BC tissues [66], GC [67] PT involved in infiltration of macrophages to the tumor microenvironment [67], involved in EMT related to re-organization of cell-cell contact [68] overexpression was correlated with poor overall survival and a more aggressive phenotype in GC [67] [72] and regulation of mitochondrial membrane remodeling [73] highly upregulated in BC, CRC tissue and cell lines [72] PT positive correlation with p-Akt and CDK2 [72], c-Jun [73] important in tumorigenesis, poor prognosis in ER+, ER+PR+, HER2+, and TNBC, inhibits apoptosis by activation of c-Jun and Bcl-3 [73] [74] overexpressed in various cancers: PCa [74], pancreatic ductal carcinoma [75], ovarian carcinoma [76]; downregulated in human cervical carcinoma cells [77] PT OXPHOS; lipid metabolism signaling [74]; downregulation could induce EMT [77] aggressive tumor progression, poor prognosis, increases cell proliferation, growth, colony formation, migration, invasion, and cell cycle [74]; silencing induces reduction of cell proliferation, invasion, migration, and enhances apoptosis [76], cells being unable to grow or proliferate in response to extracellular growth factors [78] LAMTOR3/ MAPKSP1/MP1 Late endosomal/lysosomal adaptor, MAPK and MTOR activator 3/mitogen activated protein kinase scaffold protein 1/MEK partner 1 member of the Ragulator complex involved in multiple signaling pathways that acts as a scaffold protein complex [79] overexpressed in ER+ and ER-BC cell lines and in non-tumorigenic mammary epithelial cell lines [80]; downregulated in KIRC [79] PT considered to be a convergence point for MAPK and mTOR pathways [81]; targeting MEK1/MP1/ERK1/BCL2 axis may improve clinical outcome of MLCC patients [82] required for pro-survival signaling from PI3K/AKT pathway in ER+ BC cells [80]; upregulation induces BCL2 expression (anti-apoptotic protein)…”

Section: Ptbp1/hnrnp1mentioning

confidence: 99%

Two-Dimensional-PAGE Coupled with nLC-MS/MS-Based Identification of Differentially Expressed Proteins and Tumorigenic Pathways in MCF7 Breast Cancer Cells Transfected for JTB Protein Silencing

Jayathirtha,

Jayaweera,

Whitham

et al. 2023

Molecules

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The identification of new cancer-associated genes/proteins, the characterization of their expression variation, the interactomics-based assessment of differentially expressed genes/proteins (DEGs/DEPs), and understanding the tumorigenic pathways and biological processes involved in BC genesis and progression are necessary and possible by the rapid and recent advances in bioinformatics and molecular profiling strategies. Taking into account the opinion of other authors, as well as based on our own team’s in vitro studies, we suggest that the human jumping translocation breakpoint (hJTB) protein might be considered as a tumor biomarker for BC and should be studied as a target for BC therapy. In this study, we identify DEPs, carcinogenic pathways, and biological processes associated with JTB silencing, using 2D-PAGE coupled with nano-liquid chromatography tandem mass spectrometry (nLC-MS/MS) proteomics applied to a MCF7 breast cancer cell line, for complementing and completing our previous results based on SDS-PAGE, as well as in-solution proteomics of MCF7 cells transfected for JTB downregulation. The functions of significant DEPs are analyzed using GSEA and KEGG analyses. Almost all DEPs exert pro-tumorigenic effects in the JTBlow condition, sustaining the tumor suppressive function of JTB. Thus, the identified DEPs are involved in several signaling and metabolic pathways that play pro-tumorigenic roles: EMT, ERK/MAPK, PI3K/AKT, Wnt/β-catenin, mTOR, C-MYC, NF-κB, IFN-γ and IFN-α responses, UPR, and glycolysis/gluconeogenesis. These pathways sustain cancer cell growth, adhesion, survival, proliferation, invasion, metastasis, resistance to apoptosis, tight junctions and cytoskeleton reorganization, the maintenance of stemness, metabolic reprogramming, survival in a hostile environment, and sustain a poor clinical outcome. In conclusion, JTB silencing might increase the neoplastic phenotype and behavior of the MCF7 BC cell line. The data is available via ProteomeXchange with the identifier PXD046265.

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LAMTOR3 is a prognostic biomarker in kidney renal clear cell carcinoma

Gong

et al. 2022

Clinical Laboratory Analysis

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Objective The objective of the study was to investigate the expression of LAMTOR3 in kidney renal clear cell carcinoma (KIRC) and its clinical significance. Methods The expression of LAMTOR3 in KIRC and its relationship with clinical features were analyzed using the UALCAN online database. The results were verified using KIRC gene chip data and clinical specimens. The prognosis of KIRC patients was analyzed with the GEPIA2 database. GO, KEGG, and GSEA analyses were conducted to analyze the possible molecular mechanism of LAMTOR3 in KIRC. Immunohistochemical (IHC) and hematoxylin and eosin (H&E) staining were used for histopathological detection. Results UALCAN database analysis showed that LAMTOR3 expression in KIRC was significantly lower than in normal kidney tissues and correlated with the clinical stage, pathological grade, and tumor genotype ( p < .05). GSE53757 dataset analysis consistently showed that the expression of LAMTOR3 in KIRC was significantly lower than in normal kidney tissues ( p < .01). GEPIA2 database analysis indicated that patients with low LAMTOR3 expression had poor overall and disease‐free survival rates. GSEA analysis suggested that LAMTOR3 positively regulated the citrate cycle and drug metabolism cytochrome P450 and negatively regulated folate biosynthesis and olfactory transduction. The expression of LAMTOR3 in KIRC was also significantly correlated with immune cell infiltration. Finally, IHC showed that LAMTOR3 expression in the KIRC tissues was lower than in the adjacent normal tissues. Conclusion LAMTOR3 expression is significantly lower in KIRC. LAMTOR3 may be a potential marker for KIRC diagnosis and therapy.

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LAMTOR3 is a prognostic biomarker in kidney renal clear cell carcinoma

Cited by 4 publications

References 23 publications

Immunogenic profiling of metastatic uveal melanoma discerns a potential signature related to prognosis

Immunogenic profiling of metastatic uveal melanoma discerns a potential signature related to prognosis

Two-Dimensional-PAGE Coupled with nLC-MS/MS-Based Identification of Differentially Expressed Proteins and Tumorigenic Pathways in MCF7 Breast Cancer Cells Transfected for JTB Protein Silencing

LAMTOR3 is a prognostic biomarker in kidney renal clear cell carcinoma

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