Land plants identification in forensic botany: Multigene barcoding approach

Ferri, Gianmarco; Alù, Milena; Corradini, Beatrice; Angot, Andrée; Beduschi, Giovanni

doi:10.1016/j.fsigss.2007.10.023

Cited by 11 publications

(8 citation statements)

References 2 publications

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“…However, there are also some drawbacks of using universal markers for real forensic cases. First, poor coverage of plant species in the sequence databases, namely incomplete reference data, likely limits the application of the genetic approach in botanical forensics (20,21). Second, ITS sequences are not homogenized in some species (e.g., P. rhoeas , C. ambigua , and C. incisa in our study).…”

Section: Discussionmentioning

confidence: 99%

An Assessment of the Utility of Universal and Specific Genetic Markers for Opium Poppy Identification

Lee

Hwang

Kim

et al. 2010

Journal of Forensic Sciences

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The proper identification of illicit plants such as Papaver somniferum L (opium poppy) is important for law enforcement agencies. The identification of opium poppy was presently tested using 10 genetic markers that are universal for all plants or specific to a few poppy plants. The genetic distances of universal markers such as nuclear internal transcribed spacer (ITS), 18S rRNA, plastid rbcL, and trnL-trnF intergenic spacer (IGS) of 14 species included in the Papaveraceae and Fumariaceae family were acquired by sequence comparisons. Both the ITS region and trnL-trnF IGS showed high levels of interspecific divergence. Six Papaver genera-specific markers were developed from coding regions involved in morphine biosynthesis. Three markers (TYDC, NCS, and BBE) produced amplicons only in opium poppy, providing a presence/absence test for opium poppy, while three additional markers (CYP80B1, SAT, and COR) were genus specific. These 10 markers might be useful for the forensic DNA analysis of opium poppy.

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Section: Discussionmentioning

confidence: 99%

An Assessment of the Utility of Universal and Specific Genetic Markers for Opium Poppy Identification

Lee

Hwang

Kim

et al. 2010

Journal of Forensic Sciences

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“…But the lack of discriminatory power of rbcL and primer universality for matK subjects it to further improvement (Hollingsworth et al ., ). Other combinations particularly rbcL and trnH – psbA , the internal transcribed spacers of nuclear ribosomal DNA ( nrITS/nrITS2 ) have also been evaluated for their potential (Ferri et al ., ; Kress and Erickson, ; Kress et al ., ; Pang et al ., ; Tripathi et al ., ). It has been observed that in case of two loci, the conserved coding locus will align well with the taxa of a community sample to establish deep phylogenetic branches, while the hypervariable region of the DNA barcode will align with ease in the subclades of closely related species.…”

Section: Dna Barcoding: a Genomics‐based Modern Tool For Plant Authenmentioning

confidence: 99%

DNA barcoding: an efficient tool to overcome authentication challenges in the herbal market

Mishra

Kumar

Nagireddy

et al. 2015

Plant Biotechnology Journal

279

181

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biological reference material herbal barcode library. SummaryThe past couple of decades have witnessed global resurgence of herbal-based health care. As a result, the trade of raw drugs has surged globally. Accurate and fast scientific identification of the plant(s) is the key to success for the herbal drug industry. The conventional approach is to engage an expert taxonomist, who uses a mix of traditional and modern techniques for precise plant identification. However, for bulk identification at industrial scale, the process is protracted and time-consuming. DNA barcoding, on the other hand, offers an alternative and feasible taxonomic tool box for rapid and robust species identification. For the success of DNA barcode, the barcode loci must have sufficient information to differentiate unambiguously between closely related plant species and discover new cryptic species. For herbal plant identification, matK, rbcL, trnH-psbA, ITS, trnL-F, 5S-rRNA and 18S-rRNA have been used as successful DNA barcodes. Emerging advances in DNA barcoding coupled with next-generation sequencing and high-resolution melting curve analysis have paved the way for successful species-level resolution recovered from finished herbal products. Further, development of multilocus strategy and its application has provided new vistas to the DNA barcode-based plant identification for herbal drug industry. For successful and acceptable identification of herbal ingredients and a holistic quality control of the drug, DNA barcoding needs to work harmoniously with other components of the systems biology approach. We suggest that for effectively resolving authentication challenges associated with the herbal market, DNA barcoding must be used in conjunction with metabolomics along with need-based transcriptomics and proteomics.

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“…DNA barcodes can not only resolve phylogenies of plant taxa but are also useful in ecological forensics such as the tracking of illegal trade in plant products (Kress et al, 2015). Other applications of a DNA barcode include monitoring of exotic dispersion, conservation impact assessments, authentication of parts used in preparation of herbal medicine and botanical pesticides, such as tree barks, fruits and leaves (Ferri et al, 2008;Kritpetcharat et al, 2011;Mankga et al, 2013;Mishra et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

A DNA-barcode for Melia volkensii Grke (Meliaceae) and its phylogenetic relationship with some economically important relatives

et al. 2016

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The study reports the first DNA-barcode and molecular phylogeny of the East African endemic tree species Melia volkensii using the standard two-locus plant barcoding genes (rbcL and matK). The two genes were amplified and the PCR products sequenced. Complete coding sequences were obtained for both genes. The edited and aligned sequences had lengths of 1371 bp for rbcL and 1524 bp for matK. These DNA sequences were deposited into the DNA Data Bank of Japan (DDBJ) with cross-listing in the European Molecular Biology Labaratory (EMBL) and GenBank databases. The deposited gene sequences were then subjected to separate nucleotide BLASTs in NCBI's GenBank database. Out of 100 Blast results in which the query (M. volkensii) had 96-100 percentage similarity in nucleotide sequence for the rbcL gene and 90-100% similarity for the matK gene, only 16 taxa had data for both rbcL and matK genes. These 16 taxa were used for the phylogenetic analysis and comprised of 6, 9 and 1 taxa respectively from the families Meliaceae, Simaroubaceae and Rutaceae. The barcode allowed adequate discrimination of the taxa into their respective generic and species clades. Availability of a barcode for M. volkensii will ease identification of the species, provide more robust phylogenetic reconstructions and allow for better tracking of its exotic dispersal.

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Land plants identification in forensic botany: Multigene barcoding approach

Cited by 11 publications

References 2 publications

An Assessment of the Utility of Universal and Specific Genetic Markers for Opium Poppy Identification

An Assessment of the Utility of Universal and Specific Genetic Markers for Opium Poppy Identification

DNA barcoding: an efficient tool to overcome authentication challenges in the herbal market

A DNA-barcode for Melia volkensii Grke (Meliaceae) and its phylogenetic relationship with some economically important relatives

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