Changes in the territorial pattern of the Beibu Gulf, an environmentally sensitive and ecologically fragile area in China, will directly or indirectly affect the regional ecological environment, while profoundly influencing economic development and human well-being. Therefore, it is significant to understand the ecological response in the process of territorial space changes in the Beibu Gulf to promote the coordination between sea and land and sustainable regional development. This paper used remote sensing image interpretation to generate land-use maps in 2000, 2010 and 2020, and then analyzed the spatial and temporal evolution of the territorial pattern of the Beibu Gulf from 2000 to 2020. Finally, this paper proposed a comprehensive carrying capacity evaluation system and explored the spatial functional zones of the coastal areas of the Beibu Gulf. The results showed that the demand for urban development and ecological protection between 2000 and 2020 increased built-up land and forestland by 386.71% and 25.56%, respectively, and reduced farmland by 28.33%. There was significant spatial heterogeneity in various land-use types. Where forestland is mainly distributed in the west, farmland is mainly distributed in the east, wetland is mainly distributed in the south, and orchards are spread throughout the whole area. The evaluation results of land resources, water resources and ecological conditions in the Beibu Gulf area showed that its comprehensive carrying capacity was high in the south and low in the north, and high in the west and low in the east. On this basis, this paper considered the actual situation of natural resources, ecological conditions, socio-economic development, protection and development in coastal areas; divided the study area into four categories: developed areas, priority development areas, ecological reserve areas and coastal reserve areas; and put forward corresponding control suggestions. The results of this paper could provide a scientific basis for regional development and territorial spatial planning in the coastal areas.