Lanostane triterpenes from Ganoderma lucidum suppress the adipogenesis in 3T3-L1 cells through down-regulation of SREBP-1c

Lee, Ik‐Soo; Kim, Jin-Pyo; Ryoo, In‐Ja; Kim, Young‐Hee; Choo, Soo‐Jin; Yoo, Ick Dong; Min, Byung‐Sun; Na, MinKyun; Hattori, Masao; Bae, KiHwan

doi:10.1016/j.bmcl.2010.06.093

Cited by 37 publications

(23 citation statements)

References 23 publications

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“…Adipogenesis is the process of preadipocyte differentiation to mature adipocyte, accompanied by coordinated changes in cell morphology, gene expression and hormone sensitivity [16]. The 3T3-L1 cell line has been used for identifying key molecular markers, transcription factors and various interactions that are required for adipogenesis [17-19], and is thus very useful to rapidly screen and assess the adipogenic potential of various agents.…”

Section: Discussionmentioning

confidence: 99%

“…They suggested that SREBP pathway can be a potential therapeutic target to treat obesity related metabolic diseases. The inhibitions of SREBP-1c mRNA expression were accompanied by a sharp reduction in the mRNA expression of SREBP-1c target genes, such as FAS and ACC [16]. We examined the effect of pear pomace water extract on the expression of FAS protein and observed pear pomace water extract to suppress the expression of FAS.…”

Section: Discussionmentioning

confidence: 99%

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Pear pomace water extract inhibits adipogenesis and induces apoptosis in 3T3-L1 adipocytes

et al. 2014

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Obesity occurs when a person's calorie intake exceeds the amount of energy burns, which may lead to pathologic growth of adipocytes and the accumulation of fat in the tissues. In this study, the effect and mechanism of pear pomace extracts on 3T3-L1 adipocyte differentiation and apoptosis of mature adipocytes were investigated. The effects of pear pomace extract on cell viability and the anti-adipogenic and proapoptotic effects were investigated via MTT assay, Oil red O staining, western blot analysis and apoptosis assay. 3T3-L1 preadipocytes were stimulated with DMEM containing 10% FBS, 0.5 mM 3-isobutyl-1-methylxanthine (IBMX), 5 µg/ml insulin and 1 µM dexamethasone for differentiation to adipocytes. 3T3-L1 cells were cultured with PBS or water extract of pear pomace. Water extract of pear pomace effectively inhibited lipid accumulations and expressions of PPAR-γ and C/EBPα in 3T3-L1 cells. It also increased expression of p-AMPK and decreased the expression of SREBP-1c and FAS in 3T3-L1 cells. The induction of apoptosis was observed in 3T3-L1 cells treated with pear pomace. These results indicate that pear pomace water extract inhibits adipogenesis and induces apoptosis of adipocytes and thus can be used as a potential therapeutic substance as part of prevention or treatment strategy for obesity.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Pear pomace water extract inhibits adipogenesis and induces apoptosis in 3T3-L1 adipocytes

et al. 2014

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“…demonstrated that some purified triterpenes from G. lucidum inhibit adipocyte differentiation, decrease lipid accumulation and down-regulate expression of PPAR-γ, SREBP-1c, C/EBP-α, FAS and ACC [73-75]. Although the identification of biologically active components from G. lucidum is important for their possible therapeutic use, and more than 130 triterpenes have been isolated and new triterpenes continue to be identified [76], the yield of a majority of purified triterpenes is generally low.…”

Section: Discussionmentioning

confidence: 99%

ReishiMax, mushroom based dietary supplement, inhibits adipocyte differentiation, stimulates glucose uptake and activates AMPK

Thyagarajan‐Sahu

Lane

Slíva

2011

BMC Complement Altern Med

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BackgroundObesity is a health hazard which is closely associated with various complications including insulin resistance, hypertension, dyslipidemia, atherosclerosis, type 2 diabetes and cancer. In spite of numerous preclinical and clinical interventions, the prevalence of obesity and its related disorders are on the rise demanding an urgent need for exploring novel therapeutic agents that can regulate adipogenesis. In the present study, we evaluated whether a dietary supplement ReishiMax (RM), containing triterpenes and polysaccharides extracted from medicinal mushroom Ganoderma lucidum, affects adipocyte differentiation and glucose uptake in 3T3-L1 cells.Methods3T3-L1 pre-adipocytes were differentiated into adipocytes and treated with RM (0-300 μg/ml). Adipocyte differentiation/lipid uptake was evaluated by oil red O staining and triglyceride and glycerol concentrations were determined. Gene expression was evaluated by semi-quantitative RT-PCR and Western blot analysis. Glucose uptake was determined with [3H]-glucose.ResultsRM inhibited adipocyte differentiation through the suppresion of expression of adipogenic transcription factors peroxisome proliferator-activated receptor-γ (PPAR-γ), sterol regulatory element binding element protein-1c (SREBP-1c) and CCAAT/enhancer binding protein-α (C/EBP-α). RM also suppressed expression of enzymes and proteins responsible for lipid synthesis, transport and storage: fatty acid synthase (FAS), acyl-CoA synthetase-1 (ACS1), fatty acid binding protein-4 (FABP4), fatty acid transport protein-1 (FATP1) and perilipin. RM induced AMP-activated protein kinase (AMPK) and increased glucose uptake by adipocytes.ConclusionOur study suggests that RM can control adipocyte differentiation and glucose uptake. The health benefits of ReishiMax warrant further clinical studies.

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“…To date, approximately 400 compounds have been obtained, including polysaccharides, triterpenoids, nucleotides, steroids, fatty acids, proteins, and peptides (Akihisa et al, 2005(Akihisa et al, , 2007Chien et al, 2004;Iwatsuki et al, 2003;Kawagishi et al, 1993;Luo and Lin, 2002;Luo et al, 2015;Wu et al, 2001). Among these, polysaccharides and triterpenoids were identified as the major bioactive substances in G. lucidum (Bao et al, 2002;Chien et al, 2004;Grienke et al, 2011;Kohda et al, 1985;Lee et al, 2010;Min et al, 2000;Sato et al, 2009;Pan et al, 2012). In particular, pharmacological effects of the triterpenoids from G. lucidum have increasingly been reported.…”

Section: Introductionmentioning

confidence: 97%

Isolation and identification of oxygenated lanostane-type triterpenoids from the fungus Ganoderma lucidum

Zhao

Zhang

Deng

et al. 2016

Phytochemistry Letters

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Lanostane triterpenes from Ganoderma lucidum suppress the adipogenesis in 3T3-L1 cells through down-regulation of SREBP-1c

Cited by 37 publications

References 23 publications

Pear pomace water extract inhibits adipogenesis and induces apoptosis in 3T3-L1 adipocytes

Pear pomace water extract inhibits adipogenesis and induces apoptosis in 3T3-L1 adipocytes

ReishiMax, mushroom based dietary supplement, inhibits adipocyte differentiation, stimulates glucose uptake and activates AMPK

Isolation and identification of oxygenated lanostane-type triterpenoids from the fungus Ganoderma lucidum

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