Lanthanide Hydroxide Nanoparticles Induce Angiogenesis via ROS‐Sensitive Signaling

Abstract

Recent studies suggest that the nanorods consisting of europium hydroxide could promote angiogenesis. In this study, we sought to determine if additional types of nanoparticles were capable of enhancing angiogenesis and in addition, understand the underlying mechanisms. For this reason, we employed a method that combines a high throughput in vitro cell based screen coupled with an in vivo validation using vascular specific green fluorescent protein (GFP) reporter transgenic zebrafish for examining proangiogenesis activity. After screening multiple types of nanoparticles, we discovered that four of them: EuIII(OH)3 rods (Eu Rods), EuIII(OH)3 spheres (Eu Spheres), TbIII(OH)3 rods (Tb Rods) and TbIII(OH)3 spheres (Tb Spheres), were the most effective in promoting angiogenesis. We also showed that ionic forms of europium nitrate [Eu(NO3)3] (Eu) and terbium nitrate [Tb(NO3)3] (Tb), the two lanthanide elements for these four nanoparticles, were also capable of enhancing angiogenesis. However, this effect was further enhanced by nanoparticle synthesis. Finally, we demonstrated that reactive oxygen species H2O2 is a key factor in the process of proangiogenesis by lanthanide elemental nanoparticles.

Photocontrolled Multidirectional Differentiation of Mesenchymal Stem Cells on an Upconversion Substrate

“…There is thus an urgent need to develop alternative strategies to control stem‐cell differentiation. Engineering cell‐culture matrices is a good way approaching this issue because the complex signaling pathways of stem cells can be regulated by the physicochemical properties of the extracellular matrix, such as surface composition, adhesive ligands, topography, smoothness/roughness, and flexibility/rigidity . To date, some natural materials, such as alginate and hyaluronic acid and synthetic materials,, have been developed as matrices.…”

Multi-hierarchical profiling the structure-activity relationships of engineered nanomaterials at nano-bio interfaces

“…Given that various nanorods such as CeO 2 , AlOOH, and lanthanide materials or nanoplates (e.g., Ag nanoplates) were demonstrated to be more reactive than other shapes 25 – 27 , we synthesized a series of Fe 2 O 3 nanoparticles with different morphologies and sizes, including four hexagonal nanoplates (P1~P4) with controlled diameters and thicknesses, and four nanorods (R1~R4) with systematically tuned lengths and diameters. Transmission electron microscopy (TEM) was used to determine the size and morphology of all Fe 2 O 3 particles.…”

Insights into the angiogenic effects of nanomaterials: mechanisms involved and potential applications

“…Nanomaterials, such as nano zinc oxide [110][111][112], lanthanide nanoparticles [113], and europium hydroxide [114], promote the migration and early tube formation of HUVECs. These nanomaterials activate kinases such as Akt, extracellular regulated protein kinases (ERK) 1/2, mitogen-activated protein kinase (MAPK) p38 [115] and eNOS, which are ROS-dependent, even though the production of ROS is always associated with cytotoxicity.…”