Large-area manufacturable active matrix digital microfluidics platform for high-throughput biosample handling

Ma, Hanbin; Shi, Subao; Jin, Kai; Wang, D.; Hu, Siyi; Su, Youfeng; Zhang, Y.; Li, J.; Liu, Z.; Jiang, Chen; Feng, Linrun; Guo, Xiaojun; Nathan, Arokia

doi:10.1109/iedm13553.2020.9372110

Cited by 27 publications

(21 citation statements)

References 8 publications

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“…Here, the DMF bottom plate is the electrode backplane based on an active matrix technology. The AM digital microfluidic chip integrates thin film transistors (TFT) as active switches into each pixel electrode, thus achieving independent pixel electrode control in a large-scale array [8]. And it realizes droplet manipulation by peripheral driving system.…”

Section: 1dmf Chip Design and Fabricationmentioning

confidence: 99%

A digital microfluidic system with integrated electrochemical impedance detection arrays

Jin

Huang

et al. 2022

J. Phys.: Conf. Ser.

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Electrochemical sensing provides a new way for miniaturization and low cost of equipment. Meanwhile, digital microfluidic (DMF) technology is making lab-on-chip a reality. The two technologies are essential in the point-of-care area. Electrochemical detection on electrodes and DMF-based droplet driving electrode realize their respective functions by means of electrical signals. In terms of working mode, it seems to have certain similarity, and electrochemical sensing seems to be more suitable for integrating digital microfluidic systems. We thus integrated electrochemical sensing on an active matrix (AM) DMF system. As a common transparent conductive glass, indium tin oxide (ITO) has been widely used as the top plate of the digital microfluidic chip. In this work, we used ITO glass with patterned interdigital electrodes as the top plate of the AM-DMF chip. We achieved on-chip droplet volume detection by electrochemical impedance spectroscopy (EIS). We also proposed a novel sample dilution method on the DMF chip. It takes only 30 seconds to dilute solutions into 4 different concentrations. We then analyzed droplet samples of different concentrations with EIS method. And the correlation coefficient of the fitted curve is as high as 0.9964. In conclusion, EIS is a powerful detection technology for fast, high-integration and low-cost detection on DMF system. The integration of EIS and DMF can be used in various biochemical analyses in the future.

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Section: 1dmf Chip Design and Fabricationmentioning

confidence: 99%

A digital microfluidic system with integrated electrochemical impedance detection arrays

Jin

Huang

et al. 2022

J. Phys.: Conf. Ser.

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“…6B), and achieved 75 times increase in SERS signals. Although microdroplet manipulation in most commercial instruments still relies on EWOD, such as digital PCR machine and liquid lens [99–101], researchers have been working on the implement of magnetic particles and magnetic pole matrix in integrated platforms for microdroplet preparation and screening [102–105].…”

Section: Applicationsmentioning

confidence: 99%

“…A. E. Saliba et al [39] designed a 3D spatial cell capture device based on magnetic beads, and performed culture, sorting, morphological characterization, and immunophenotyping of T-and B-lymphocyte cells. This process is useful for rapid instruments still relies on EWOD, such as digital PCR machine and liquid lens [99][100][101], researchers have been working on the implement of magnetic particles and magnetic pole matrix in integrated platforms for microdroplet preparation and screening [102][103][104][105].…”

Section: Cell Manipulationmentioning

confidence: 99%

Recent advances in magnetic digital microfluidic platforms

Cheng

Liu

et al. 2021

Electrophoresis

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Magnetic Digital microfluidics (DMF), which enables the manipulation of droplets containing different types of samples and reagents by permanent magnets or electromagnet arrays, has been used as a promising platform technology for bioanalytical and preparative assays. This is due to its unique advantages such as simple and “power free” operation, easy assembly, great compatibility with auto control systems, and dual functionality of magnetic particles (actuation and target attachment). Over the past decades, magnetic DMF technique has gained a widespread attention in many fields such as sample‐to‐answer molecular diagnostics, immunoassays, cell assays, on‐demand chemical synthesis, and single‐cell manipulation. In the first part of this review, we summarised features of magnetic DMF. Then, we introduced the actuation mechanisms and fabrication of magnetic DMF. Furthermore, we discussed five main applications of magnetic DMF, namely drug screening, protein assays, polymerase chain reaction (PCR), cell manipulation, and chemical analysis and synthesis. In the last part of the review, current challenges and limitations with magnetic DMF technique were discussed, such as biocompatibility, automation of microdroplet control systems, and microdroplet evaporation, with an eye on towards future development.

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“…31,32 Active-matrix (AM) technology, which integrates thin-film transistors into each pixel to achieve pixel control in a large array, has been widely used in flat panel displays. 12 Thus, AM technology in EWOD would be an ideal solution to overcome the limitation of the passive electrode array. 33,34 A few research studies have applied AM-DMF in molecular diagnosis, 35,36 and there have been a few reports on its application in the study of single-cell applications.…”

Section: ■ Introductionmentioning

confidence: 99%

Large-Area Electronics-Enabled High-Resolution Digital Microfluidics for Parallel Single-Cell Manipulation

Ye²,

Shi³

et al. 2023

Anal. Chem.

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Large-area electronics as switching elements are an ideal option for electrode-array-based digital microfluidics. With support of highly scalable thin-film semiconductor technology, high-resolution digital droplets (diameter around 100 μm) containing single-cell samples can be manipulated freely on a two-dimensional plane with programmable addressing logic. In addition, single-cell generation and manipulation as foundations for single-cell research demand ease of operation, multifunctionality, and accurate tools. In this work, we reported an active-matrix digital microfluidic platform for single-cell generation and manipulation. The active device contained 26,368 electrodes that could be independently addressed to perform parallel and simultaneous droplet generation and achieved single-cell manipulation. We demonstrate a high-resolution digital droplet generation with a droplet volume limit of 500 pL and show the continuous and stable movement of droplet-contained cells for over 1 h. Furthermore, the success rate of single droplet formation was higher than 98%, generating tens of single cells within 10 s. In addition, a pristine single-cell generation rate of 29% was achieved without further selection procedures, and the droplets containing single cells could then be tested for on-chip cell culturing. After 20 h of culturing, about 12.5% of the single cells showed cell proliferation.

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Large-area manufacturable active matrix digital microfluidics platform for high-throughput biosample handling

Cited by 27 publications

References 8 publications

A digital microfluidic system with integrated electrochemical impedance detection arrays

A digital microfluidic system with integrated electrochemical impedance detection arrays

Recent advances in magnetic digital microfluidic platforms

Large-Area Electronics-Enabled High-Resolution Digital Microfluidics for Parallel Single-Cell Manipulation

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