Large area sub-micron chemical imaging of magnesium in sea urchin teeth

Mašić, Admir; Weaver, James C.

doi:10.1016/j.jsb.2014.12.005

Cited by 24 publications

(16 citation statements)

References 40 publications

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“…The broad bands (band width in the range of 30 cm −1 , see Supporting Information, Figure S3) associated with the phosphate and carbonate vibrations imply that these phases are amorphous, thus corresponding to ACP and ACC, respectively . Furthermore, the position of the ACC Raman peak suggests around 30% magnesium (Mg) substitution in the carbonate phase whereas the ACP peak position indicates that the phosphate phase is fluorinated in the form of amorphous FAP . In the inner layers, the intensities of mineral phase bands are much smaller and similar to those arising from the organic matrix.…”

Section: Resultsmentioning

confidence: 99%

The Mantis Shrimp Saddle: A Biological Spring Combining Stiffness and Flexibility

Tadayon

Amini

Mašić

et al. 2015

Adv Funct Materials

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Stomatopods are aggressive crustacean predators that use a pair of ultrafast raptorial appendages to strike on prey. This swift movement is driven by a power amplification system comprising components that must be able to repetitively store and release a high amount of elastic energy. An essential component of this system is the saddle structure, in which the elastic energy is stored by bending prior to striking. Here, a comprehensive study that sheds light on the microstructural and chemical designs of the stomatopod's saddle is conducted. MicroCT scans combined with electron microscopy imaging, elemental mapping, high‐resolution confocal Raman microscopy, and nanomechanical mapping show that the saddle is a bilayer structure with sharp changes in chemical composition and mechanical properties between the layers. The outer layer is heavily mineralized whereas the inner layer contains a high content of chitin and proteins, leading to a spatial organization of phases which is optimized for load distribution during saddle bending. The mineralized outer layer sustains compressive stresses, whereas the inner biopolymeric layer provides tensile resistance. These findings reveal that the saddle chemical composition and microstructure have been spatially tuned to generate a stiff, yet flexible structure that is optimized for storage of elastic energy.

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Section: Resultsmentioning

confidence: 99%

The Mantis Shrimp Saddle: A Biological Spring Combining Stiffness and Flexibility

Tadayon

Amini

Mašić

et al. 2015

Adv Funct Materials

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“…The accelerating voltage was 20 kV and the working distance kept constant at 15 mm. This system provided 16 bit BSE images, thus providing much better gray level resolution and detail compared to the more commonly used 8 bit file format (Masic and Weaver, 2015). Additionally, the microscope combined large fields of view with high in-plane resolution, which significantly streamlined the image acquisition process.…”

Section: Backscattered Electron (Be) Imagingmentioning

confidence: 99%

Osteocyte lacunar properties in rat cortical bone: Differences between lamellar and central bone

Bach-Gansmo

Weaver²,

Jensen

et al. 2015

Journal of Structural Biology

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“…EDS systems have also been developed with multiple detectors to largely eliminate shadowing artifacts, which are frequently a problem encountered when acquiring element maps from irregular surfaces with a single X-ray detector. By harnessing the combined strengths of multi-detector EDS and TrueSurface Raman spectroscopy in the study of topographically complex sea urchin teeth, for example, the element distribution of dried sample surfaces could be measured either using EDS under vacuum, or in the case of native hydrated samples, the location of the Raman v 1 CO 3 2- could be used for measuring the extent of Mg substitution in the calcite crystal lattice [37].…”

Section: Introductionmentioning

confidence: 99%

Large-scale micron-order 3D surface correlative chemical imaging of ancient Roman concrete

Maragh

Weaver²,

Mašić

2019

PLoS ONE

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There has been significant progress in recent years aimed at the development of new analytical techniques for investigating structure-function relationships in hierarchically ordered materials. Inspired by these technological advances and the potential for applying these approaches to the study of construction materials from antiquity, we present a new set of high throughput characterization tools for investigating ancient Roman concrete, which like many ancient construction materials, exhibits compositional heterogeneity and structural complexity across multiple length scales. The detailed characterization of ancient Roman concrete at each of these scales is important for understanding its mechanics, resilience, degradation pathways, and for making informed decisions regarding its preservation. In this multi-scale characterization investigation of ancient Roman concrete samples collected from the ancient city of Privernum (Priverno, Italy), cm-scale maps with micron-scale features were collected using multi-detector energy dispersive spectroscopy (EDS) and confocal Raman microscopy on both polished cross-sections and topographically complex fracture surfaces to extract both bulk and surface information. Raman spectroscopy was used for chemical profiling and phase characterization, and data collected using EDS was used to construct ternary diagrams to supplement our understanding of the different phases. We also present a methodology for correlating data collected using different techniques on the same sample at different orientations, which shows remarkable potential in using complementary characterization approaches in the study of heterogeneous materials with complex surface topographies.

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Large area sub-micron chemical imaging of magnesium in sea urchin teeth

Cited by 24 publications

References 40 publications

The Mantis Shrimp Saddle: A Biological Spring Combining Stiffness and Flexibility

The Mantis Shrimp Saddle: A Biological Spring Combining Stiffness and Flexibility

Osteocyte lacunar properties in rat cortical bone: Differences between lamellar and central bone

Large-scale micron-order 3D surface correlative chemical imaging of ancient Roman concrete

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