Large-depth three-photon fluorescence microscopy imaging of cortical microvasculature on nonhuman primates with bright AIE probe In vivo

Zhang, Hequn; Fu, Peng; Liu, Yin; Zhang, Zheng; Zhu, Liang; Wang, Mengqi; Abdellah, Marwan; He, Mubin; Qian, Jun; Roe, Anna Wang; Wang, Xi

doi:10.1016/j.biomaterials.2022.121809

Cited by 12 publications

(6 citation statements)

References 53 publications

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“…Moreover, the upgraded NIR‐II microscope enhanced vascular edge recognition in contrast to the DE method (Figure S7, Supporting Information), thereby facilitating the precise extraction of physiological information related to vascular morphological structures. [ 54 ] This advancement holds promise for promoting future research on associated disease processes. Derived from the above results, our high optical throughput intravital microscope was proved as a reliable, accurate, and powerful tool that can monitor the accurate and subtle variations in cerebrovascular structures, and hopefully, it could be applied to the study on Alzheimer's disease, vascular disorders, and other related areas.…”

Section: Resultsmentioning

confidence: 99%

Enhancing Total Optical Throughput of Microscopy with Deep Learning for Intravital Observation

et al. 2023

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The significance of performing large‐depth dynamic microscopic imaging in vivo for life science research cannot be overstated. However, the optical throughput of the microscope limits the available information per unit of time, i.e., it is difficult to obtain both high spatial and temporal resolution at once. Here, a method is proposed to construct a kind of intravital microscopy with high optical throughput, by making near‐infrared‐II (NIR‐II, 900–1880 nm) wide‐field fluorescence microscopy learn from two‐photon fluorescence microscopy based on a scale‐recurrent network. Using this upgraded NIR‐II fluorescence microscope, vessels in the opaque brain of a rodent are reconstructed three‐dimensionally. Five‐fold axial and thirteen‐fold lateral resolution improvements are achieved without sacrificing temporal resolution and light utilization. Also, tiny cerebral vessel dilatations in early acute respiratory failure mice are observed, with this high optical throughput NIR‐II microscope at an imaging speed of 30 fps.

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Section: Resultsmentioning

confidence: 99%

Enhancing Total Optical Throughput of Microscopy with Deep Learning for Intravital Observation

et al. 2023

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“…The NIR-II fluorescence microscopic monitoring through-thinned-skulls of nonhuman primates brain vessels using microscopic monitoring and the NIR-IIb fluorescence GI imaging was successfully achieved (Figure A and B) . Centimeter-deep and three-photon fluorescence NIR-II vascular imaging in nonhuman primates has also been realized. , Further, a series of quantitative approaches have been developed to assess the structural changes of deep vessels using biocompatible AIE dots . The morphological changes and organization remodeling of cortical blood vessels in marmosets during stroke have been fully and accurately analyzed. , …”

Section: Light Up Life: See the Unseenmentioning

confidence: 99%

“…255 Centimeter-deep and three-photon fluorescence NIR-II vascular imaging in nonhuman primates has also been realized. 256,257 Further, a series of quantitative approaches have been developed to assess the structural changes of deep vessels using biocompatible AIE dots. 258 The morphological changes and organization remodeling of cortical blood vessels in marmosets during stroke have been fully and accurately analyzed.…”

Section: Near-infrared Imagingmentioning

confidence: 99%

Aggregation-Induced Emission (AIE), Life and Health

et al. 2023

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Light has profoundly impacted modern medicine and healthcare, with numerous luminescent agents and imaging techniques currently being used to assess health and treat diseases. As an emerging concept in luminescence, aggregation-induced emission (AIE) has shown great potential in biological applications due to its advantages in terms of brightness, biocompatibility, photostability, and positive correlation with concentration. This review provides a comprehensive summary of AIE luminogens applied in imaging of biological structure and dynamic physiological processes, disease diagnosis and treatment, and detection and monitoring of specific analytes, followed by representative works. Discussions on critical issues and perspectives on future directions are also included. This review aims to stimulate the interest of researchers from different fields, including chemistry, biology, materials science, medicine, etc., thus promoting the development of AIE in the fields of life and health.

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“…14H and I). 180 Compared to conventional fluorescent compounds, AIEgens show great advantages in the imaging of CVDs, including the long wavelength emission in the NIR-IIb region that promotes the bioimaging of deep tissues, high QYs that promote fluorescence intensity, high clarity, and enhanced SNRs, especially in cerebral angiography, [189][190][191][192][193][194][195] expand the progresses of CVDs. Hence, they should be applied for bioimaging in more animal and non-cancer disease models.…”

Section: Cardiovascular Disease Bioimagingmentioning

confidence: 99%