Large dose hyperpolarized water with dissolution-DNP at high magnetic field

Abstract

We demonstrate a method for the preparation of hyperpolarized water by dissolution Dynamic Nuclear Polarization at high magnetic field. Protons were polarized at 6.7T and 1.1K to >70% with frequency modulated microwave irradiation at 188GHz. 97.2±0.7% of the radical was extracted from the sample in the dissolution in a two-phase system. 16±1mL of 5.0M H in DO with a polarization of 13.0±0.9% in the liquid state was obtained, corresponding to an enhancement factor of 4000±300 compared to the thermal equilibrium at 9.4T and 293K. A longitudinal relaxation time constant of 16±1s was measured. The sample was polarized and dissolved in a fluid path compatible with clinical polarizers. The volume of hyperpolarized water produced by this method enables angiography and perfusion measurements in large animals, as well as NMR experiments for studies of e.g. proton exchange and polarization transfer to other nuclei.

Liquid-State 13C Polarization of 30% through Photoinduced Nonpersistent Radicals

“…[19][20][21][22] For the clinical use of HP substrates, the radicals should preferably be removed from the HP solution. 10,[23][24] Furthermore, the radical, although beneficial for the DNP process, reduces the nuclear T1, after the dissolution step.…”

Dynamic coronary MR angiography in a pig model with hyperpolarized water

“…The images in this study were obtained with 5% proton polarization. We believe that 10 times higher polarization is achievable , which would improve the image quality dramatically over what is presented here. The higher polarization could be obtained by reducing the relaxation loss in dissolution by more efficient removal of the radical, and by automated sample transfer.…”

“…The sample preparation followed the methods published in . A sample of 1 mL 30 mM TEMPO (2,2,6,6‐Tetramethylpiperidine 1‐oxyl, 98%) in H 2 O/glycerol 1:1 (w/w) was prepared.…”

Sensitivity-enhanced three-dimensional and carbon-detected two-dimensional NMR of proteins using hyperpolarized water

“…The acquisition of protein spectra thus often extends to hours or days, which can be prohibitive for the study of systems which are evolving rapidly or have limited stability in solution. We have recently explored the use of hyperpolarized water (Lipso et al 2017) to boost signals in protein spectra. Hyperpolarized HDO produced using dissolution-dynamic nuclear polarization (D-DNP) can serve as a polarization reservoir, enhancing the 1 H signals of sites undergoing chemical-or polarization exchange with the hyperpolarized solvent.…”