Large field of view correction by using conjugate adaptive optics with multiple guide stars

Zhao, Qi; Shi, Xin; Zhu, Xinpei; Zheng, Yao; Wu, Chenxue; Tang, Hengjie; Hu, Ling; Xue, Ying; Gong, Wei; Si, Ke

doi:10.1002/jbio.201800225

Cited by 9 publications

(4 citation statements)

References 34 publications

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“…In this paper, we chose coherent optical adaptive technique (COAT) to implement wavefront shaping, which was originally introduced to compensate the atmospheric turbulence and then applied for both optical focusing, deep tissue imaging and optogenetic regulation . As we all known, the amount of light scattering is related to the light wavelength.…”

Section: Introductionmentioning

confidence: 99%

Reliability of wavefront shaping based on coherent optical adaptive technique in deep tissue focusing

et al. 2019

Journal of Biophotonics

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Wavefront shaping can compensate the wavefront distortions in deep tissue focusing, leading to an improved penetration depth. However, when using the backscattered signals as the feedback, unexpected compensation bias may be introduced, resulting in focusing position deviations or even no focus in the illumination focal plane. Here we investigated the reliability of wavefront shaping based on coherent optical adaptive technique in deep tissue focusing by measuring the position deviations between the foci in the illumination focal plane and the epi‐detection plane. The experimental results show that when the penetration depth reaches 150 μm in mouse brain tissue (with scattering coefficient ~22.42 mm−1) using a 488 nm laser and an objective lens with 0.75 numerical aperture, the center of the real focus will deviate out of one radius range of the Airy disk while the optimized focus in the epi‐detection plane maintained basically at the center. With the penetration depth increases, the peak to background ratio of the focus in the illumination focal plane decreases faster than that in the epi‐detection plane. The results indicate that when the penetration depth reaches 150 μm, feedback based on backscattered signals will make wavefront shaping lose its reliability, which may provide a guidance for applications of non‐invasive precise optogenetics or deep tissue optical stimulation using wavefront shaping methods. A, Intensity distribution in the epi‐detection plane and the illumination focal plane before and after correction, corresponding to brain sections with 250 and 300 μm thickness, respectively. Scale bar is 2 μm. B, Averaged focusing deviations in the epi‐detection plane (optimized) and the illumination focal plane (monitored) after compensation. The unit of the ordinate is one Airy disk diameter. Black dashed line represents one Airy disk radius. Bars represent the SE of each measurement set.

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Section: Introductionmentioning

confidence: 99%

Reliability of wavefront shaping based on coherent optical adaptive technique in deep tissue focusing

et al. 2019

Journal of Biophotonics

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“…As illustrated in Figure A, in pupil AO, SLM is placed on the conjugate plane of the objective rear pupil; while in conjugate AO, SLM is placed on the conjugate planes of the scattering medium, shown in Figure B. For the conjugate AO system, SLM is conjugate to the source of the optical aberrations, which provides a remarkable advantage in FOV . Therefore, as for the vortex beam, whose focus is doughnut‐shaped, conjugate AO may be a better choice to achieve the fast and flexible correction and improve spatial resolution.…”

Section: The Conjugate Ao Correction System For Doughnut Beam Based Omentioning

confidence: 99%

“…Here we use COAT algorithm for the aberration compensation (Figure S1). The correction process can be divided into two steps, where the phase elements on SLM are randomly divided into two half parts (Figure S1A) . In the first step, one‐half of the phase elements remain constant as a reference and the other half are modulated at different frequencies (Figure S1C).…”

Section: The Conjugate Ao Correction System For Doughnut Beam Based Omentioning

confidence: 99%

“…Therefore, the corrected field of view (FOV) of pupil AO is limited to a small region around the central guide star. On the contrary, when the correction element is placed on the conjugate plane of the sample, which we called conjugate AO, the aberrations varying with spatial positions can also be compensated and thus the corrected FOV is enhanced .…”

Section: Introductionmentioning

confidence: 99%

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Aberration corrections of doughnut beam by adaptive optics in the turbid medium

Chen

et al. 2019

Journal of Biophotonics

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The doughnut beam is a spatially structured beam which has been widely used in super-resolution microscopy, laser trapping and so on. However, when it passes through thick scattering medium, aberrations will seriously affect its performance. Currently, adaptive optics (AO) has become one of the most powerful tools to compensate aberrations. However, conventional AO always suffers from limited corrected field of view (FOV). Here, we propose a method with conjugate AO system based on coherent optical adaptive technique. The results show that the corrected FOV can be improved effectively. For a wide range of the optical applications with doughnut beam, our method has potentials in correcting aberrations with high speed in turbid media.(A) Mouse brain slice, (B) the distribution of r PAO , (C) the distribution of r CAO . The vortex beam focus of the blue point in (B) and (C) among a 137.5 × 137.5 μm FOV (D1) ideally, (D2) with scattering, (D3) in pupil AO system and (D4) in conjugate AO system. K E Y W O R D S aberration correction, adaptive optics, doughnut beam, turbid medium

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Optogenetics

Si¹

2023

Biomedical Photonic Technologies

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Large field of view correction by using conjugate adaptive optics with multiple guide stars

Cited by 9 publications

References 34 publications

Reliability of wavefront shaping based on coherent optical adaptive technique in deep tissue focusing

Reliability of wavefront shaping based on coherent optical adaptive technique in deep tissue focusing

Aberration corrections of doughnut beam by adaptive optics in the turbid medium

Optogenetics

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