Large genetic distances among Aedes aegypti populations along the South Pacific coast of Mexico.

García-Franco, Francisco; Muñoz, Marı́a de Lourdes; Lozano-Fuentes, Saúl; Fernández‐Salas, Ildefonso; Garcia-Rejon, Julián E.; Beaty, Barry J.; Black, William C.

doi:10.4269/ajtmh.2002.66.594

Cited by 39 publications

(40 citation statements)

References 24 publications

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“…aegypti in México; Vazeille and others 75 for Ae. albopictus in Madagascar; García-Franco and others 76 for Ae. aegypti in other Mexican populations; Bosio and others 65 for Ae.…”

Section: Discussionmentioning

confidence: 95%

Genetic Structure of Anopheles (Nyssorhynchus) marajoara (Diptera: Culicidae) in Colombia

Brochero

Liu²,

Wilkerson³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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Abstract. Five Anopheles marajoara Galvão and Damasceno populations, representing diverse ecological conditions, were sampled throughout Colombia and analyzed using nine hypervariable DNA microsatellite loci. The overall genetic diversity ( H = 0.58) was lower than that determined for some Brazilian populations using the same markers. The Caquetá population (Colombia) had the lowest gene diversity ( H = 0.48), and it was the only population at Hardy-Weinberg equilibrium. Hardy-Weinberg disequilibrium in the remaining four populations was probably caused by the Wahlund effect. The assignment analyses showed two incompletely isolated gene pools separated by the Eastern Andean cordillera. However, other possible geographical barriers (rivers and other mountains) did not play any role in the moderate genetic heterogeneity found among these populations ( F ST = 0.069). These results are noteworthy, because this species is a putative malaria vector in Colombia.

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“…aegypti in México; Vazeille and others 75 for Ae. albopictus in Madagascar; García-Franco and others 76 for Ae. aegypti in other Mexican populations; Bosio and others 65 for Ae.…”

Section: Discussionmentioning

confidence: 95%

Genetic Structure of Anopheles (Nyssorhynchus) marajoara (Diptera: Culicidae) in Colombia

Brochero

Liu²,

Wilkerson³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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“…García-Franco et al, 2002), Argentina and Uruguay (de Sousa et al, 2001;Julio et al, 2009;Soliani et al, 2010), and Puerto Rico (Apostol et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

Genetic structure of Aedes aegypti populations determined using pairwise comparisons

Patarro¹,

Guirado²,

Ravazzi³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. The biological characteristics of Aedes aegypti (Diptera, Culicidae), which is a vector of dengue and yellow fever, make this organism a good model for studying population structure and the events that may influence it under the effect of human activity. We assessed the genetic variability of five A. aegypti populations using RAPD-PCR technique and six primers. Four populations were from Brazil and one was from the USA. A total of 165 polymorphic DNA loci were generated. Considering the six primers and the five populations, the mean value of inter-population genetic diversity (Gst) was 0.277, which is considered high according to the Wright classification. However, pairwise comparisons of the populations gave variable Gst values ranging from 0.044 to 0.289. This variation followed the population's geographic distance to some extent but was also influenced by human activity. The lowest Gst values were obtained in the comparison of populations from cities with intensive commercial and medical contacts. These mosquito populations were previously classified as insecticide resistant, susceptible, or with decreased susceptibility; this parameter apparently had an effect on the Gst values obtained in the pairwise comparisons.

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“…Estudos populacionais de insetos também têm utilizado essa técnica para examinar a estrutura de cruzamento (Su et al 2003), construção eficiente de mapas de ligação em populações de A. aegypti ou estudos taxonômicos de insetos, principalmente em nível intra-específico (Antolin et al 1996) e fluxo gênico (Garcia-Franco et al 2002).…”

Section: Discussionunclassified

Variabilidade Genética em Populações de Aedes aegypti (L.) (Diptera: Culicidae) Utilizando Marcadores de RAPD

Hiragi¹,

Simões²,

Martins³

et al. 2009

Neotrop. entomol.

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-Aedes aegypti (L.) is an important vector of diseases such as the yellow fever and dengue, present in tropical and subtropical regions. The objective of this study was to analyze the genetic variability of different A. aegypti populations using RAPD (Random Amplified Polymorphic DNA) markers as a basic study to support the use of biocontrol strategies. DNA of ten collected larvae from three different populations were analyzed using ten RAPD primers. The results indicated the existence of genetic variability inter and intrapopulation. This was confirmed by a dendrogram that grouped the populations in two main clusters with a genetic similarity of 24%. In one of these clusters, it was possible to distinguish two populations that showed 50% similarity. The molecular variance analysis indicated that the interpopulation genetic diversity (55,01%) was higher than the intrapopulation genetic diversity (44,99%). A high genetic polymorphism (H t = 0.2656) and high levels of genetic differentiation between populations (G st = 0.3689) were found. The adopted DNA extraction protocol proved to be efficient regardless the insect development stage used, avoiding the addition of reagents or additional stages of processing. Future experiments can be performed to confirm if the detected variability is related to the resistance characteristics of each population to a determined pesticide.KEY WORDS: Dengue vector, population genetic, resistance RESUMO -Aedes aegypti (L.) é vetor de importantes doenças como a febre amarela e a dengue, presentes em regiões tropicais e subtropicais. Para o sucesso no seu controle biológico é importante conhecer a estrutura genética e os mecanismos que resultaram na diversidade das populações. O objetivo deste estudo foi analisar a variabilidade genética de diferentes populações de A. aegypti utilizando marcadores de RAPD (Polimorfismo de DNA amplificado ao acaso). DNA de dez larvas coletadas a partir de três populações de diferentes localidades foi analisado usando dez iniciadores de RAPD. Os resultados indicaram a existência de variabilidade genética inter e intrapopulacional. Isso foi confirmado por um dendrograma que agrupou as populações em dois blocos principais com similaridade genética de 24%. Em um desses agrupamentos foi possível distinguir duas populações que apresentaram grau de similaridade de 50%. A diversidade genética entre as populações (55,01%) foi mais elevada que a diversidade genética dentro das populações (44,99%) aplicando-se análise por AMOVA. Altos níveis de polimorfismo genético (H t = 0.2656) e de diferenciação genética entre as populações (G st = 0.3689) foram observados. Além disso, o protocolo de extração de DNA adotado mostrou-se eficiente para a análise do inseto independente do seu estágio de desenvolvimento, evitando-se o acréscimo de reagentes ou etapas adicionais de processamento. Futuros experimentos poderão ser realizados para confirmar se a variabilidade observada pode estar ligada às características de resistência de cada população a um determinado pest...

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Large genetic distances among Aedes aegypti populations along the South Pacific coast of Mexico.

Cited by 39 publications

References 24 publications

Genetic Structure of Anopheles (Nyssorhynchus) marajoara (Diptera: Culicidae) in Colombia

Genetic Structure of Anopheles (Nyssorhynchus) marajoara (Diptera: Culicidae) in Colombia

Genetic structure of Aedes aegypti populations determined using pairwise comparisons

Variabilidade Genética em Populações de Aedes aegypti (L.) (Diptera: Culicidae) Utilizando Marcadores de RAPD

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