Large increases in attractant concentration disrupt the polar localization of bacterial chemoreceptors

Lamanna, A; Ordal, George; Kiessling, Laura L.

doi:10.1111/j.1365-2958.2005.04728.x

Cited by 49 publications

(64 citation statements)

References 82 publications

(148 reference statements)

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“…It has been reported that Escherichia coli exhibits metabolism-dependent chemotaxis towards glycerol [26]. Rhodobacter sphaeroides shows chemotaxis towards a wide range of amino acids, organic acids, and sugars [27]. Metabolism-dependent chemotaxis is similar to other bacterial behavioral responses collectively known as energy taxis, defined as a behavioral response to stimuli affecting cellular energy levels [28].…”

Section: Resultsmentioning

confidence: 99%

Metabolism Dependent Chemotaxis of Pseudomonas aeruginosa N1 Towards Anionic Detergent Sodium Dodecyl Sulfate

Chaturvedi

Kumar

2013

Indian J Microbiol

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Sodium dodecyl sulfate (SDS) is one of the most commonly used detergent, which exhibits excellent biocidal activity against various bacteria and fungi. It is commonly employed in many detergent formulations and is employed for disinfection purposes. It is shown to be toxic to fishes, aquatic animals and is also inhibitory to microbes and cyanobacteria. We had isolated a strain belonging to Pseudomonas aeruginosa N1, from a detergent contaminated pond situated in Varanasi city India, which was able to degrade and metabolize SDS as a source of carbon. In the present investigation, we have studied chemotactic response of this strain towards SDS. The results clearly indicate that this strain showed chemotactic response towards SDS. The nature of chemotaxis was found to be metabolism dependent as glucose grown cells showed a delayed chemotactic response towards SDS. This is first study that reported chemotaxis response for P. aeruginosa towards anionic detergent SDS.

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Section: Resultsmentioning

confidence: 99%

Metabolism Dependent Chemotaxis of Pseudomonas aeruginosa N1 Towards Anionic Detergent Sodium Dodecyl Sulfate

Chaturvedi

Kumar

2013

Indian J Microbiol

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“…Specifically, the diffuse staining previously observed is likely due to the formation of lateral clusters. As a consequence, we were able to extend and refine the observations concerning McpB localization made by Lamanna et al (12).…”

Section: Attractant Binding Reduces the Number Of Polar Receptor Clusmentioning

confidence: 86%

“…Our approach was similar to the one employed by Lamanna et al (12), although we refined their analysis to take into account recent observations in E. coli regarding the positioning of the receptor clusters. Although the majority of receptors are localized at the poles in E. coli, many are also found in distinct clusters laterally along the length of the cell (7-9, 37, 39 -41).…”

Section: Attractant Binding Reduces the Number Of Polar Receptor Clusmentioning

confidence: 99%

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Attractant Binding Induces Distinct Structural Changes to the Polar and Lateral Signaling Clusters in Bacillus subtilis Chemotaxis

Wu¹,

Walukiewicz

Glekas

et al. 2011

Journal of Biological Chemistry

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Bacteria employ a modified two-component system for chemotaxis, where the receptors form ternary complexes with CheA histidine kinases and CheW adaptor proteins. These complexes are arranged in semi-ordered arrays clustered predominantly at the cell poles. The prevailing models assume that these arrays are static and reorganize only locally in response to attractant binding. Recent studies have shown, however, that these structures may in fact be much more fluid. We investigated the localization of the chemotaxis signaling arrays in Bacillus subtilis using immunofluorescence and live cell fluorescence microscopy. We found that the receptors were localized in clusters at the poles in most cells. However, when the cells were exposed to attractant, the number exhibiting polar clusters was reduced roughly 2-fold, whereas the number exhibiting lateral clusters distinct from the poles increased significantly. These changes in receptor clustering were reversible as polar localization was reestablished in adapted cells. We also investigated the dynamic localization of CheV, a hybrid protein consisting of an N-terminal CheW-like adaptor domain and a C-terminal response regulator domain that is known to be phosphorylated by CheA, using immunofluorescence. Interestingly, we found that CheV was localized predominantly at lateral clusters in unstimulated cells. However, upon exposure to attractant, CheV was found to be predominantly localized to the cell poles. Moreover, changes in CheV localization are phosphorylation-dependent. Collectively, these results suggest that the chemotaxis signaling arrays in B. subtilis are dynamic structures and that feedback loops involving phosphorylation may regulate the positioning of individual proteins.Many motile bacteria employ for chemotaxis a modified two-component system to sense and respond to chemicals, where the receptors form ternary complexes with the CheA histidine kinase and the CheW adaptor protein (1, 2). The clustering of these ternary complexes into semi-ordered hexagonal lattices has been documented in multiple species (3) and is presumably conserved in all chemotactic bacteria where the three proteins are found. These arrays are thought to amplify the response to attractant binding (4,5). A number of models have specifically proposed that cooperative interactions between the receptors within these arrays enable bacteria to sense small differences in the number of attractant-bound receptors over a wide range of concentrations (see Ref. 6).Multiple studies have investigated the structure and molecular determinants of these clusters (e.g. Refs. 7 and 8) along with their role in signal transduction. In Escherichia coli, the receptors form mixed trimers of receptor homodimers. These trimers are believed to form the basic building blocks for the larger clusters, which range in size from tens to thousands of receptors (9). These clusters are found predominantly at the cell poles, although they are also found along the lateral length of the cell. Attractant binding, which inhibi...

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“…Although the interpretation of these results is far from simple, they do seem congruent with a previous study in which cells were exposed to high concentrations of attractant. Once again, the chemotaxis receptors were dispersed (17).…”

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confidence: 99%

How the “Melting” and “Freezing” of Protein Molecules May Be Used in Cell Signaling

Bray

Williams

2008

ACS Chem. Biol.

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The motile response of Escherichia coli bacteria to attractants and repellents is one of the best-understood examples of a signal transduction pathway. A number of recent studies suggest that the receptors in this system undergo major changes in both their degree of structural order and their state of aggregation in the membrane. We discuss the thermodynamic basis for this effect and argue that the “freezing” or “melting” of protein structure may be the language of signaling.

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Large increases in attractant concentration disrupt the polar localization of bacterial chemoreceptors

Cited by 49 publications

References 82 publications

Metabolism Dependent Chemotaxis of Pseudomonas aeruginosa N1 Towards Anionic Detergent Sodium Dodecyl Sulfate

Metabolism Dependent Chemotaxis of Pseudomonas aeruginosa N1 Towards Anionic Detergent Sodium Dodecyl Sulfate

Attractant Binding Induces Distinct Structural Changes to the Polar and Lateral Signaling Clusters in Bacillus subtilis Chemotaxis

How the “Melting” and “Freezing” of Protein Molecules May Be Used in Cell Signaling

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