Large scale artificial rearing of Anastrepha sp.1 aff. fraterculus (Diptera: Tephritidae) in Brazil

Abstract

Some species of the genus Anastrepha (Diptera: Tephritidae) are successfully managed by matching the sterile insect technique with parasitoid releases. Such strategies used in integrated pest management can be implemented only where insect mass-rearing programs are feasible. In this study, we show the process of domestication, rearing technology and quality control data obtained from 54 generations of Anastrepha sp.1 aff. fraterculus (Wiedemann, 1830) kept under fully artificial conditions. Eggs were collected by an artificial oviposition panel consisting of one side of the cage made of blue voile fabric externally covered with a thin layer of silicon rubber. They were then air-bubbled in water at 25 ºC for 48 h before seeding. Larvae were reared on the regular laboratory artificial diet with 66 % of agar reduction turning over a semi-liquid diet, which reduced costs and improved insect quality. The adult and larval diets were composed of local ingredients including hydrolyzed yeast. When large-scale production of this fly is contemplated, the critical stage is larval development. This system of artificial rearing for A. fraterculus sp.1 developed in Brazil, allows for the production of a large number of insects of excellent quality using local ingredients and less agar in diet composition than the original medium used for this species. By reducing the interval of egg collection, the system might be optimized in terms of insect yield and, therefore, meet the demands of A. fraterculus sp.1 with regard to integrated pest management purposes.

An integrative multidisciplinary approach to understanding cryptic divergence in Brazilian species of theAnastrepha fraterculuscomplex (Diptera: Tephritidae)

“…1 aff. fraterculus by Walder et al (2014), our study showed that the PIR population is behaviourally, genetically, and morphologically different from the populations previously classified as either morphotype Brazilian-1 (Hern andez-Ortiz et al, 2004 or Anastrepha sp. 1 aff.…”

“…Interestingly, studies evaluating A. fraterculus populations from Piracicaba have found flies in at least three hosts: Eugenia pyriformis , a.k.a. uvaia (Walder et al ., ); Malpighia emarginata , a.k.a. Barbados cherry (B. J. Paranhos, personal communication), and P. guajava , a.k.a.…”

Isolation of entomopathogenic nematodes in an apple orchard in Southern Brazil and its virulence to Anastrepha fraterculus (Diptera: Tephritidae) larvae, under laboratory conditions

“…An A. fraterculus population from Cena (Centro de Energia Nuclear na Agricultura, Piracicaba -Brazil), was reared following Walder et al (2014) to obtain late third-instar larvae. They were used in an experiment completely random designed, with five repetitions composed by a nine-centimeter Petri dishes (Rohde et al, 2012) with 12 larvae, in a dark climatic chamber (24±2 °C, U.R.…”

Interspecific Competition Between Native and Exotic Fruit Fly Parasitoidsin Mixed Orchards in Maceio, Alagoas, Brazil

“…The trays were covered with a dark fabric, to simulate the conditions inside the fruit, and placed in shelves at room temperature (28 to 30°C) and relative humidity (85 to 95%). The diet and methodology used was based on Walder et al (2014). Collections of larvae were performed when they were about to pupate, in the third larval stage, separating them from the diets by washing in water and filtering in a plastic sieve; 30% of these larvae was used to maintain the breeding of fruit flies and 70% were subjected to parasitism.…”