Large-scale in vitro embryo production and pregnancy rates from Bos taurus, Bos indicus, and indicus-taurus dairy cows using sexed sperm

Pontes, J. H. F.; Silva, K.C.F.; Basso, Andrea; Rigo, Adelheid; Ferreira, Christina R.; Santos, Gustavo Martins Gomes dos; Sanches, B. V.; Porcionato, J.P.F.; Vieira, Priscila H.; Faifer, F.S.; Sterza, Fabiana de Andrade Melo; Schenk, J.L.; Seneda, Marcelo Marcondes

doi:10.1016/j.theriogenology.2010.06.004

Cited by 165 publications

(151 citation statements)

References 15 publications

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“…These results should be carefully interpreted, as ET numbers cannot ensure fully conclusive data. However, our pregnancy rates are higher than those reported by other authors [24,27,62,63].…”

Section: Discussioncontrasting

confidence: 88%

In vitro and in vivo quality of bovine embryos in vitro produced with sex-sorted sperm

et al. 2012

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In this work we analyzed the effects of three culture systems on developmental ability of bovine embryos in vitro produced with sexed sperm, the survival to vitrification (cryologic vitrification method) of such blastocysts, and their pregnancy rates after embryo transfer to recipients, both as fresh and after vitrification/warming. Finally, we measured the accuracy of the sorting protocol by a polymerase chain reaction-based method to validate the embryo sex at blastocyst stages. We confirmed an individual effect of the bull as well as development rates of embryos produced with sorted sperm lower than embryos with unsorted sperm, independent of the culture system used. The cryoresistance to vitrification of embryos produced with sexed sperm did not differ from that of conventionally produced embryos (re-expansion rates at 24 and 48 h: 74.6% vs. 75.5%, and 64.5% vs. 68.1% for embryos produced with conventional and sorted sperm, respectively; hatching rates at 48 h: 63.55% vs. 55.5% for embryos produced with conventional and sorted sperm, respectively). Finally, no significant differences were found in pregnancy rates after the embryo transfer of fresh and vitrified/warmed blastocysts (52.8% vs. 42.0%, respectively; P Ͼ 0.05). Male and female embryos produced with sorted sperm showed the same quality in terms of developmental ability, cryoresistance, and pregnancy rates after transfer. Our culture system, coupled with the vitrification in fiber plugs, provides good quality sex-known embryos which survive vitrification at similar rates than embryos produced with conventional unsorted sperm; also it produces good pregnancy rates after transfer of sexed embryos both fresh and after vitrification and warming.

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Section: Discussioncontrasting

confidence: 88%

In vitro and in vivo quality of bovine embryos in vitro produced with sex-sorted sperm

et al. 2012

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“…The increase in dairy IVEP was primarily supported by the commercial availability of sex-sorted semen (Pontes et al, 2010) and caused important changes in the features of the Brazilian embryo market. The most significant one was a shift towards the use of B. taurus breeds for IVEP (Fig.…”

Section: A Summary Of Changes In the Brazilian Embryo Industry Duringmentioning

confidence: 99%

Brazilian embryo industry in context: pitfalls, lessons, and expectations for the future

Viana¹,

Figueiredo²,

Siqueira³

2017

Anim Reprod

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The aim of this review is to provide a brief description of the current situation of the embryo industry in Brazil, in the context of the national and international livestock production scenario. Total number of embryos produced (375,894) declined in 2015 in comparison with the previous years. B. taurus dairy breeds and crossbreds accounted for 51.8% of the total embryo production. Moreover, the percentage of frozen-tawed embryos transferred reached 22.8% in 2015, the highest value in a decade. A greater proportion of embryos were produced in vitro, both in dairy (97.2%) and beef (90.2%) breeds. The use of embryo technologies in Brazil has remarkably increased 726.5% in the past 20 years, but still represents only 0.33% of the number of cows and heifers at reproductive age. Nonetheless, embryo transfer (ET) accounted for an estimated 19.7% of all purebred calves born and registered by the Brazilian Zebu Cattle Breeders Association in the period 2005-2015, highlighting the importance of ET for animal breeding and genetic improvement of the herd. In the world´s context, Brazil is the largest producer of bovine in vitro produced (IVP) embryos, but is ranked only 11th based on an index of intensity of use of embryo technologies, below Canada, USA and various European countries. This scenario demonstrates a potential for further increases in embryo production in Brazil, mainly associated with the expected adoption of new technologies by a large proportion of dairy and beef farms; the use of embryo technologies for large scale production of crossbreds; and to eventual increases in the international embryo import/export activity.

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“…In livestock production embryos are transferred mainly at the age of 7 days after IVF (Pontes et al, 2010) with the highest pregnancy rates obtained after transfer of day 7 expanded blastocysts (Hasler et al, 1997). Thus, ability of day 7 IVP bovine embryos at different developmental stages to produce steady survival rates after vitrification in hollow fibers holds special interest in regard to the practical application.…”

Section: Introductionmentioning

confidence: 99%

A new simple and reliable vitrification device based on Hollow Fiber Vitrification (HFV) method evaluated using IVP bovine embryos

et al. 2017

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A new vitrification device based on hollow fiber vitrification (HFV) was constructed using a glass capillary, which lead to simplified construction process and increased practicality of the device. The hollow fiber was attached to heat-pulled tip of the glass capillary using forceps. A protective sheath fitted on the capillary provided protection for the cellulose triacetate hollow fiber with loaded embryos and allowed safe storage in liquid nitrogen for long periods of time (2-12 month), transfer between tanks with liquid nitrogen and transportation within these tanks. No embryos were lost in the process. The device was tested using seven-dayold and eight-day-old IVP bovine blastocysts and expanded blastocysts as a model. Obtained survival (90% at 24 h post warming) and hatching rates (62% at 72 h post warming) of day 7 blastocysts and expanded blastocysts were comparable to those gained using various vitrification carriers. Vitrified embryos did not show an increase in the number of cells with damaged membrane or a decrease in total cell number per embryos in comparison to their non-vitrified counterparts. Day 7 and 8 expanded blastocysts did not differ significantly in terms of survival at 24 (97.01 vs. 97.50%) and 48 h post warming (95.52 vs. 95%), but showed significantly higher survival and hatching rates than day 7 and 8 blastocysts. These results indicated that high and repeatable survival rates can be obtained by selection of IVP bovine embryos at the developmental stage of expanded blastocyst for HFV. Further modification of the method may be required to achieve high and stable results with different developmental stages of IVP bovine embryo. The vitrification device presented in the current article may contribute to wider application of HFV method in livestock production.

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Large-scale in vitro embryo production and pregnancy rates from Bos taurus, Bos indicus, and indicus-taurus dairy cows using sexed sperm

Cited by 165 publications

References 15 publications

In vitro and in vivo quality of bovine embryos in vitro produced with sex-sorted sperm

In vitro and in vivo quality of bovine embryos in vitro produced with sex-sorted sperm

Brazilian embryo industry in context: pitfalls, lessons, and expectations for the future

A new simple and reliable vitrification device based on Hollow Fiber Vitrification (HFV) method evaluated using IVP bovine embryos

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