Large-scale quantification of human osteocyte lacunar morphological biomarkers as assessed by ultra-high-resolution desktop micro-computed tomography

Goff, Elliott; Buccino, Federica; Bregoli, Chiara; McKinley, Jonathan P.; Aeppli, Basil; Recker, Robert R.; Shane, Elizabeth; Cohen, Adi; Kuhn, Gisela; Müller, Ralph

doi:10.1016/j.bone.2021.116094

Cited by 25 publications

(19 citation statements)

References 52 publications

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“…Focused ion beam/scanning electron microscopy (FIB/SEM), confocal laser scanning microscopy, or synchrotron radiation computed tomography are especially used for these purposes. Due to certain limitations of technologies mentioned above, which include mainly depth range and lower availability, ultra-high-resolution micro-CT technology might be more advantageous [ 78 ]. Moreover, recent approaches allow the use of synchrotron scans to quantify bone stress in combination with morphological differences of the lacunar-canalicular network, where bone alterations such as micro-damages can be detected using artificial intelligence [ 79 ].…”

Section: Discussionmentioning

confidence: 99%

Long-Term Cola Intake Does Not Cause Evident Pathological Alterations in the Femoral Bone Microstructure: An Animal Study in Adult Mice

et al. 2023

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Short-term animal experiments and association studies in humans have shown that cola intake may have a detrimental impact on bone mineral density (BMD); however, other bone parameters have not been investigated. This study examined the effects of long-term cola consumption on the femoral bone microstructure using adult mice (n = 32) as an animal model, which were divided into water and cola groups depending on whether they received water or cola along with a standard rodent diet for 6 months. Micro-computed tomography revealed that cola intake did not significantly affect all measured parameters characterizing trabecular bone mass and microarchitecture, as well as cortical microarchitecture and geometry in both sexes, although a slight deterioration of these parameters was noted. Cola consumption also resulted in a slightly, statistically insignificant worsening of bone mechanical properties. In contrast to female mice, males receiving cola had a lower area of primary osteons’ vascular canals. Nevertheless, long-term cola intake did not cause evident pathological alterations in the femur of adult mice, possibly due to a balanced diet and no restriction of physical activity. Therefore, the adverse effects of cola consumption on BMD, the only bone parameter studied so far, may be caused by other risk and lifestyle factors.

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Section: Discussionmentioning

confidence: 99%

Long-Term Cola Intake Does Not Cause Evident Pathological Alterations in the Femoral Bone Microstructure: An Animal Study in Adult Mice

et al. 2023

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“…At the first stage, the scanning of the sample was performed using a resolution of 8.6 µm/pixel at a voltage of 80 kV with a set of 1081 projections and 0.5 s exposition ti0me. Next, a region of interest (ROI), including bone trabeculae [ 20 ] was selected and scanned with a resolution of 1.1 μm/pixel at a voltage of 80 kV with 1441 projections and 0.5 s exposition time. The obtained data were reconstructed with the Filtered Back Projection method using the XRM Reconstructor software.…”

Section: Methodsmentioning

confidence: 99%

Biopolymer Material from Human Spongiosa for Regenerative Medicine Application

et al. 2022

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Natural biopolymers demonstrate significant bone and connective tissue-engineering application efficiency. However, the quality of the biopolymer directly depends on microstructure and biochemical properties. This study aims to investigate the biocompatibility and microstructural properties of demineralized human spongiosa Lyoplast® (Samara, Russian Federation). The graft’s microstructural and biochemical properties were analyzed by scanning electron microscopy (SEM), micro-computed tomography, Raman spectroscopy, and proteomic analysis. Furthermore, the cell adhesion property of the graft was evaluated using cell cultures and fluorescence microscopy. Microstructural analysis revealed the hierarchical porous structure of the graft with complete removal of the cellular debris and bone marrow components. Moreover, the proteomic analysis confirmed the preservation of collagen and extracellular proteins, stimulating and inhibiting cell adhesion, proliferation, and differentiation. We revealed the adhesion of chondroblast cell cultures in vitro without any evidence of cytotoxicity. According to the study results, demineralized human spongiosa Lyoplast® can be effectively used as the bioactive scaffold for articular hyaline cartilage tissue engineering.

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“…(31,83) The OLS analysis of bone sections is a 2D method, whereas a full three-dimensional (3D) assessment of osteocyte lacunar volume and shape can be achieved using X-ray tomography. (178) Goldner trichrome staining (Fig. 5D) allows to distinguish mineralized ECM (stained in green) from osteoid (stained in red) and evaluate structural parameters as well as osteoblast and osteoclast activity.…”

Section: Bone Biopsy As a Tool To Explore Bone Tissue Characteristics...mentioning

confidence: 99%

“…( 31,83 ) The OLS analysis of bone sections is a 2D method, whereas a full three‐dimensional (3D) assessment of osteocyte lacunar volume and shape can be achieved using X‐ray tomography. ( 178 )…”

Section: Bone Biopsy As a Tool To Explore Bone Tissue Characteristics...mentioning

confidence: 99%

Early-Onset Osteoporosis: Rare Monogenic Forms Elucidate the Complexity of Disease Pathogenesis Beyond Type I Collagen

Costantini

Mäkitie

Hartmann

et al. 2020

Journal of Bone and Mineral Research

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Early-onset osteoporosis (EOOP), characterized by low bone mineral density (BMD) and fractures, affects children, premenopausal women and men aged <50 years. EOOP may be secondary to a chronic illness, long-term medication, nutritional deficiencies, etc. If no such cause is identified, EOOP is regarded primary and may then be related to rare variants in genes playing a pivotal role in bone homeostasis. If the cause remains unknown, EOOP is considered idiopathic. The scope of this review is to guide through clinical and genetic diagnostics of EOOP, summarize the present knowledge on rare monogenic forms of EOOP, and describe how analysis of bone biopsy samples can lead to a better understanding of the disease pathogenesis. The diagnostic pathway of EOOP is often complicated and extensive assessments may be needed to reliably exclude secondary causes. Due to the genetic heterogeneity and overlapping features in the various genetic forms of EOOP and other bone fragility disorders, the genetic diagnosis usually requires the use of next-generation sequencing to investigate several genes simultaneously. Recent discoveries have elucidated the complexity of disease pathogenesis both regarding genetic architecture and bone tissue-level pathology. Two rare monogenic forms of EOOP are due to defects in genes partaking in the canonical WNT pathway: LRP5 and WNT1. Variants in the genes encoding plastin-3 (PLS3) and sphingomyelin synthase 2 (SGMS2) have also been found in children and young adults with skeletal fragility. The molecular mechanisms leading from gene defects to clinical manifestations are often not fully understood. Detailed analysis of patient-derived transiliac bone biopsies gives valuable information to understand disease pathogenesis, distinguishes EOOP from other bone fragility disorders, and guides in patient management, but is not widely available in clinical settings. Despite the great advances in this field, EOOP remains an insufficiently explored entity and further research is needed to optimize diagnostic and therapeutic approaches.

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Large-scale quantification of human osteocyte lacunar morphological biomarkers as assessed by ultra-high-resolution desktop micro-computed tomography

Cited by 25 publications

References 52 publications

Long-Term Cola Intake Does Not Cause Evident Pathological Alterations in the Femoral Bone Microstructure: An Animal Study in Adult Mice

Long-Term Cola Intake Does Not Cause Evident Pathological Alterations in the Femoral Bone Microstructure: An Animal Study in Adult Mice

Biopolymer Material from Human Spongiosa for Regenerative Medicine Application

Early-Onset Osteoporosis: Rare Monogenic Forms Elucidate the Complexity of Disease Pathogenesis Beyond Type I Collagen

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