Large-Scale Quantitative Proteomic Analysis during Different Stages of Somatic Embryogenesis in Larix olgensis

Abstract

Larix olgensis is an economically important tree species native to northeastern China. The use of somatic embryogenesis (SE) is efficient and enables the rapid production of varieties with desirable qualities. Here, isobaric labeling via tandem mass tags was used to conduct a large-scale quantitative proteomic analysis of proteins in three critically important stages of SE in L. olgensis: the primary embryogenic callus, the single embryo, and the cotyledon embryo. We identified 6269 proteins, including 176 shared differentially expressed proteins across the three groups. Many of these proteins are involved in glycolipid metabolism, hormone response/signal transduction, cell synthesis and differentiation, and water transport; proteins involved in stress resistance and secondary metabolism, as well as transcription factors, play key regulatory roles in SE. The results of this study provide new insights into the key pathways and proteins involved in SE in Larix. Our findings have implications for the expression of totipotency, the preparation of synthetic seeds, and genetic transformation.

Integration analysis of transcriptome and proteome profiles brings new insights of somatic embryogenesis of two eucalyptus species

“…Hesami and colleagues used transcriptome sequencing and identified 1,850 genes downregulated and 1,873 genes upregulated in embryogenic callus compared to non-embryogenic callus of cannabis [ 15 ], including 42 different TF classes (e.g., MYB, C2H2, ERF, AP2, bHLH, bZIP, B3, WRKY and GRAS ). Hou et al used LC-MS/MS (liquid chromatography with tandem mass spectrometry) and identified 6,269 differentially expressed proteins (DEPs) in three stages (the primary embryogenic callus, the single embryo, and the cotyledon embryo) of SE in Larix olgensis , including 18 TF families were identified, including 18 TFs from the AP2, NAM and WOX families [ 16 ]. Zhang et al identified 1,178 DEPs in the early SE of longan ( Dimocarpus longan ) and found that sodium butyrate (a deacetylase inhibitor) can reduce the proliferation and delay the differentiation of embryogenic callus by regulating the homeostasis of reactive oxygen species (ROS) andindole-3-acetic acid (IAA) [ 17 ].…”

Comprehensive Proteome and Acetylome Analysis of Needle Senescence in Larix gmelinii

“…However, up to date only a few studies have involved the proteomics of larch. For example, Zhang and collaborators used proteomics to study the mechanism of drought stress in Larix olgensis families [2]; Han and collaborators analyzed the formation of adventitious root development in hybrid larch with proteome [3]; and proteomics were widely used in the study of somatic embryogenesis in larch [4][5][6][7]. No studies to date have conducted a comprehensive analysis of the proteomic in needle senescence in L. gmelinii.…”