Large-scale screening identifies a novel microRNA, miR-15a-3p, which induces apoptosis in human cancer cell lines

Druz, Aliaksandr; Chen, Yu‐Chi; Guha, Rajarshi; Betenbaugh, Michael J.; Martin, Scott E.; Shiloach, Joseph

doi:10.4161/rna.23339

Cited by 43 publications

(32 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is the case for miR-1 in A549 lung cancer cells which enhances activation of caspase-3 and caspase-7 [118]. The same was demonstrated for miR-15a-3p , a novel member of the proapoptotic miRNA cluster miR-15a/16 [119]. Caspase-3, together with caspase-7, is also activated by inhibition of miR-133a/b and miR-361-3p in 95D lung cancer cell line [120].…”

Section: Mirnas Regulating Apoptosismentioning

confidence: 73%

MicroRNAs-Role in Lung Cancer

et al. 2014

View full text Add to dashboard Cite

Regulation of gene expression is essential for normal physiological functions; thus deregulation of gene expression is common in disease conditions. One level of regulation of gene expression is performed by noncoding RNAs, among which microRNAs (miRNA) are the best studied. Abnormal expression of these molecular players can lead to pathogenic processes such as heart disease, immune system abnormalities, and carcinogenesis, to name but a few. Of a length of 18–25 nucleotides miRNAs are involved in binding partial complementary sequences within the 3′-UTR (3′-untranslated region) of the target mRNAs. Depending on the type of neoplastic transformation, miRNAs can act both as oncogenes (oncomirs) or as tumor suppressors. Because of the great importance of miRNAs, most researches focus on either their role as biomarkers or their potential as therapeutic targets. Herein, we present the review of microRNA biology, function, and tumorigenic potential with emphasis on their role in lung cancer.

show abstract

Section: Mirnas Regulating Apoptosismentioning

confidence: 73%

MicroRNAs-Role in Lung Cancer

et al. 2014

View full text Add to dashboard Cite

show abstract

“…MiR-15a, a cell growth suppressor, was evaluated in human cancer cells and found to have a pro-apoptotic role by activating caspase 3/7, which reduces cell viability [32]. In addition, miR-15a has been correlated with different pathophysiological events in the liver, which are also side-effects of anabolic steroids [33].…”

Section: Discussionmentioning

confidence: 99%

Vitamin D manipulates miR-181c, miR-20b and miR-15a in human umbilical vein endothelial cells exposed to a diabetic-like environment

Zitman‐Gal

Green

Pasmanik‐Chor

et al. 2014

Cardiovasc Diabetol

View full text Add to dashboard Cite

BackgroundHigh blood and tissue concentrations of glucose and advanced glycation end-products are believed to play an important role in the development of vascular complications in patients with diabetes mellitus (DM) and chronic kidney disease. MicroRNAs (miRNA) are non-coding RNAs that regulate gene expression in a sequence specific manner. MiRNA are involved in various biological processes and become novel biomarkers, modulators and therapeutic targets for diseases such as cancer, atherosclerosis, and DM. Calcitriol (the active form of vitamin D) may inhibit endothelial proliferation, blunt angiogenesis, and be a cardioprotective agent. Calcitriol deficiency is a risk factor for DM and hypertension. The aim of this project was to study the miRNA microarray expression changes in human umbilical vein endothelial cells (HUVEC) treated in a diabetic-like environment with the addition of calcitriol.MethodsHUVEC were treated for 24 h with 200 μg/ml human serum albumin (HSA) and 100 mg/dl glucose (control group) or 200 μg/ml AGE-HSA, and 250 mg/dl glucose (diabetic-like environment), and physiological concentrations (10-10 mol/l) of calcitriol. miRNA microarray analysis and real time PCR to validate the miRNA expression profile and mRNA target gene expression were carried out.ResultsCompared to control, 31 mature human miRNA were differentially expressed in the presence of a diabetic-like environment. Addition of physiological concentrations of calcitriol revealed 39 differentially expressed mature human miRNA. MiR-181c, miR-15a, miR-20b, miR-411, miR-659, miR-126 and miR-510 were selected for further analysis because they are known to be modified in DM and in other biological disorders. The predicted targets of these miRNA (such as KLF6, KLF9, KLF10, TXNIP and IL8) correspond to molecular and biological processes such as immune and defense responses, signal transduction and regulation of RNA.ConclusionThis study identified novel miRNA in the field of diabetic vasculopathy and might provide new information about the effect of vitamin D on gene regulation induced by a diabetic-like environment. New gene targets that are part of the molecular mechanism and the therapeutic treatment in diabetic vasculopathy are highlighted.

show abstract

“…This approach has previously proven effective for apoptosis screening (Druz et al 2013a) and recombinant secreted protein screening (Fischer et al 2014; Strotbek et al 2013)in CHO cells. In this study, we developed an image-based high-throughput screening method to detect per cell green fluorescence signal, which is applied as a proxy for the number of molecules of NTSR1 protein expressed per cell.…”

Section: Discussionmentioning

confidence: 99%

“…MiRNAs have emerged as powerful tools for engineering cells with desirable properties (Jadhav et al 2013), such as improved protein production capabilities (Fischer et al 2014; Jadhav et al 2014; Loh et al 2014) and enhanced anti-apoptotic properties under stress conditions(Druz et al 2013a; Druz et al 2013b). MiRNAs are a novel class of small, non-coding RNAs that can simultaneously silence multiple genes by binding to their 3′-untranslated regions(3′-UTR)(Filipowicz et al 2008).…”

Section: Introductionmentioning

confidence: 99%

MiRNA mimic screen for improved expression of functional neurotensin receptor from HEK 293 cells

Xiao

Chen

Betenbaugh

et al. 2015

Biotech & Bioengineering

Self Cite

View full text Add to dashboard Cite

Obtaining adequate quantities of functional mammalian membrane proteins has been a bottleneck in their structural and functional studies because the expression of these proteins from mammalian cells is relatively low. To explore the possibility of enhancing expression of these proteins using miRNA, a stable T-REx-293 cell line expressing the neurotensin receptor type 1 (NTSR1), a hard-to-express G protein-coupled receptor (GPCR), was constructed. The cell line was then subjected to human miRNA mimic library screening. In parallel, an HEK293 cell line expressing luciferase was also screened with the same human miRNA mimic library. Five microRNA mimics: hsa-miR-22-5p, hsa-miR-18a-5p, hsa-miR-22-3p, hsa-miR-429 and hsa-miR-2110 were identified from both screens. They led to 48% increase in the expression of functional NTSR1 and to 239% increase of luciferase expression. These miRNAs were also effective in enhancing the expression of secreted glypican-3 hFc-fusion protein from HEK293 cells. The results indicate that these molecules may have a wide role in enhancing the production of proteins with biomedical interest.

show abstract

Large-scale screening identifies a novel microRNA, miR-15a-3p, which induces apoptosis in human cancer cell lines

Abstract: (2013) Large-scale screening identifies a novel microRNA, miR-15a-3p, which induces apoptosis in human cancer cell lines, RNA Biology, 10:2, 287-300,

Cited by 43 publications

References 62 publications

MicroRNAs-Role in Lung Cancer

MicroRNAs-Role in Lung Cancer

Vitamin D manipulates miR-181c, miR-20b and miR-15a in human umbilical vein endothelial cells exposed to a diabetic-like environment

MiRNA mimic screen for improved expression of functional neurotensin receptor from HEK 293 cells

Contact Info

Product

Resources

About