Large vesicle formation within cells induced by treatment with a mixed surfactant

Packwood, L.; Taylor, Evan L.; Storey, T.M.; Evans-Gowing, Richard; Baillie-Johnson, H.; Warn, R. M.

doi:10.1016/0968-4328(96)00019-4

Micron

1996

DOI: 10.1016/0968-4328(96)00019-4

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Large vesicle formation within cells induced by treatment with a mixed surfactant

L. Packwood

Evan L. Taylor

T.M. Storey

et al.

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Cited by 2 publications

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“…Because of their properties, FITC-dextrans are used in a wide variety of applications. They serve as markers for translational diffusion of macromolecules in the cytoplasm of cells by microinjection , and for intracellular delivery by endocytosis, , vesicle fusion, shock waves and electroporation. − They are well-adapted for monitoring the process of vesicle fusion , and vesicle formation, , for the characterization of polar solute transport across biological membranes − and for kinetic studies on drug release from delivery systems. − …”

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Characterization of Fluorescein Isothiocyanate-Dextrans Used in Vesicle Permeability Studies

Andrieux

Lesieur

et al. 2002

Anal. Chem.

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Fluorescein Isothiocyanate-dextrans of various weight average molecular masses (4,400-487,000) were analyzed in buffer solution for pH, osmolarity, fluorescence intensity as a function of the polymer concentration, average molecular masses, and radii of gyration. Labeling of polymers and conformation of the polymers were characterized by high-performance gel exclusion chromatography (HPLC-GEC) and small-angle X-ray scattering. The fluorescence measurements evidence the absence of fluorescence quenching of the FITC chromophores but the existence of an inner filter effect at high polymer concentration. The conformation of the polymers in buffer is very likely of random coil type, as shown by the relationship between the radii of gyration and the weight-average molecular masses of the dextrans (Mw). The medium used to analyze the FITC-dextrans by HPLC-GEC strongly influences their elution behavior. In buffer medium, they are sieved over the TSK G4000 PW column through a single population according to their Mw. whereas in pure water, they are separated into several species by an exclusion mechanism that depends on the number of labeled sites per dextran molecule. A Monte Carlo simulation was used to analyze the distribution of the fluorescent labels. HPLC-GEC in water could interestingly be applied to yield labeled polymers bearing a known number of functionalized groups.

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confidence: 99%

Characterization of Fluorescein Isothiocyanate-Dextrans Used in Vesicle Permeability Studies

Andrieux

Lesieur

et al. 2002

Anal. Chem.

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Time dependent physicochemical changes of SDS-CTAB interacted self assembled vesicles: Ostwald ripening effect

Mal

Ghosh

Moulik

2021

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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Large vesicle formation within cells induced by treatment with a mixed surfactant

Cited by 2 publications

References 21 publications

Characterization of Fluorescein Isothiocyanate-Dextrans Used in Vesicle Permeability Studies

Characterization of Fluorescein Isothiocyanate-Dextrans Used in Vesicle Permeability Studies

Time dependent physicochemical changes of SDS-CTAB interacted self assembled vesicles: Ostwald ripening effect

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