Larvicidal activity, route of interaction and ultrastructural changes in Aedes aegypti exposed to entomopathogenic fungi

Bitencourt, Ricardo de Oliveira Barbosa; Mallet, Jacenir Reis dos Santos; Mesquita, Emily; Gôlo, Patrícia Silva; Fiorotti, Jéssica; Bittencourt, Vânia Rita Elias Pinheiro; Pontes, Emerson Guedes; Angelo, Isabele da Costa

doi:10.1016/j.actatropica.2020.105732

Cited by 18 publications

(21 citation statements)

References 34 publications

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“…aegypti [22]. Additionally, mineral oil has been shown to enhance the efficacy of fungal propagules in the aquatic environment, demonstrating its potential as an adjuvant in entomopathogenic fungi [43]. These studies highlight the importance of selecting the appropriate form of inoculum and cultural condition for efficacious control of disease vectors.…”

Section: Factors That Influence Spore Qualitymentioning

confidence: 96%

“…When B. bassiana conidia are suspended in water, they are primarily localized in the gut, mouthparts, and perispiracular lobes of Ae. aegypti larvae [43]. Fungal conidia can obstruct the midgut of Ae.…”

Section: Invasion Through Digestive Tractmentioning

confidence: 99%

“…However, their well-developed innate immune system offers a general and rapid response to pathogen infection [50]. The insect innate immune system relies on cellular responses (phagocytosis, nodulation, and encapsulation) as well as humoral responses (antimicrobial peptides, melanization and reactive oxygen species) [43,51]. Humoral responses of mosquitoes against fungi are largely induced through Toll, JAK-STAT, and IMD pathways [52].…”

Section: Immune Response Of Mosquitomentioning

confidence: 99%

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Recent Advancements in Pathogenic Mechanisms, Applications and Strategies for Entomopathogenic Fungi in Mosquito Biocontrol

Qin

Liu

Peng

et al. 2023

JoF

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Fungal diseases are widespread among insects and play a crucial role in naturally regulating insect populations. Mosquitoes, known as vectors for numerous infectious diseases, pose a significant threat to human health. Entomopathogenic fungi (EPF) have emerged as highly promising alternative agents to chemical mosquitocides for controlling mosquitoes at all stages of their life cycle due to their unique infection pathway through direct contact with the insect’s cuticle. In recent years, significant advancements have been made in understanding the infection pathways and pathogenic mechanisms of EPF against mosquitoes. Various strategies involving the use of EPF alone or combinations with other approaches have been employed to target mosquitoes at various developmental stages. Moreover, the application of genetic technologies in fungi has opened up new avenues for enhancing the mosquitocidal efficacy of EPF. This review presents a comprehensive summary of recent advancements in our understanding the pathogenic mechanisms of EPF, their applications in mosquito management, and the combination of EPF with other approaches and employment of transgenic technologies. The biosafety concerns associated with their use and the corresponding approaches are also discussed. The recent progress suggests that EPF have the potential to serve as a future biorational tool for controlling mosquito vectors.

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Section: Factors That Influence Spore Qualitymentioning

confidence: 96%

Section: Invasion Through Digestive Tractmentioning

confidence: 99%

Section: Immune Response Of Mosquitomentioning

confidence: 99%

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Recent Advancements in Pathogenic Mechanisms, Applications and Strategies for Entomopathogenic Fungi in Mosquito Biocontrol

Qin

Liu

Peng

et al. 2023

JoF

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“…The cultures were grown on oat medium under controlled conditions [25 ± 1 • C; relative humidity (RH) ≥ 80] for 14 days. For the production of blastospores, 3 mL of conidial aqueous suspensions at 10 8 mL −1 were inoculated into 42 mL of Adamek's medium and incubated at 25 • C for 72 h. The propagules were washed using 0.01% Tween 80 (Isofar, Rio de Janeiro, Brazil) (v/v) sterile distilled water solution according to the adapted protocol described by Bitencourt et al [30]. Blastospores were quantified and adjusted to 10 6 blastospores mL −1 .…”

Section: Entomopathogenic Fungusmentioning

confidence: 99%

How Dopamine Influences Survival and Cellular Immune Response of Rhipicephalus microplus Inoculated with Metarhizium anisopliae

Corrêa

Fiorotti

Mesquita

et al. 2021

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Dopamine (DA) is a biogenic monoamine reported to modulate insect hemocytes. Although the immune functions of DA are known in insects, there is a lack of knowledge of DA’s role in the immune system of ticks. The use of Metarhizium anisopliae has been considered for tick control, driving studies on the immune response of these arthropods challenged with fungi. The present study evaluated the effect of DA on the cellular immune response and survival of Rhipicephalus microplus inoculated with M. anisopliae blastospores. Exogenous DA increased both ticks’ survival 72 h after M. anisopliae inoculation and the number of circulating hemocytes compared to the control group, 24 h after the treatment. The phagocytic index of tick hemocytes challenged with M. anisopliae did not change upon injection of exogenous DA. Phenoloxidase activity in the hemolymph of ticks injected with DA and the fungus or exclusively with DA was higher than in untreated ticks or ticks inoculated with the fungus alone, 72 h after treatment. DA was detected in the hemocytes of fungus-treated and untreated ticks. Unveiling the cellular immune response in ticks challenged with entomopathogenic fungi is important to improve strategies for the biological control of these ectoparasites.

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“…Aedes aegypti mosquitoes are the main vectors of arboviruses such as those that cause dengue, chikungunya, and Zika illnesses (Consoli and Oliveira, 1994;de Oliveira Barbosa Bitencourt et al, 2021). These diseases have occupied a prominent position in public health in several countries of the Americas, including Brazil where the occurrence of all these arboviruses has been recorded simultaneously since 2015 (Saúde and Saúde, 2016).…”

Section: Introductionmentioning

confidence: 99%

Extracts of Amazonian Fungi With Larvicidal Activities Against Aedes aegypti

et al. 2021

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The global increase in diseases transmitted by the vector Aedes aegypti, new and re-emerging, underscores the need for alternative and more effective methods of controlling mosquitoes. Our aim was to identify fungal strains from the Amazon rain forest that produce metabolites with larvicidal activity against Aedes aegypti. Thirty-six fungal strains belonging to 23 different genera of fungi, isolated from water samples collected in the state of Amazonas, Brazil were cultivated. The liquid medium was separated from the mycelium by filtration. Medium fractions were extracted with ethyl acetate and isopropanol 9:1 volume:volume, and the mycelia with ethyl acetate and methanol 1:1. The extracts were vacuum dried and the larvicidal activity was evaluated in selective bioassays containing 500 μg/ml of the dried fungal extracts. Larval mortality was evaluated up to 72 h. None of the mycelium extracts showed larvicidal activity greater than 50% at 72 h. In contrast, 15 culture medium extracts had larvicidal activity equal to or greater than 50% and eight killed more than 90% of the larvae within 72 h. These eight extracts from fungi belonging to seven different genera (Aspergillus, Cladosporium, Trichoderma, Diaporthe, Albifimbria, Emmia, and Sarocladium) were selected for the determination of LC50 and LC90. Albifimbria lateralis (1160) medium extracts presented the lowest LC50 value (0.268 μg/ml) after 24 h exposure. Diaporthe ueckerae (1203) medium extracts presented the lowest value of LC90 (2.928 μg/ml) at 24 h, the lowest values of LC50 (0.108 μg/ml) and LC90 (0.894 μg/ml) at 48 h and also at 72 h (LC50 = 0.062 μg/ml and LC90 = 0.476 μg/ml). Extracts from Al. lateralis (1160) and D. ueckerae (1203) showed potential for developing new, naturally derived products, to be applied in integrated vector management programs against Ae. aegypti.

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Larvicidal activity, route of interaction and ultrastructural changes in Aedes aegypti exposed to entomopathogenic fungi

Cited by 18 publications

References 34 publications

Recent Advancements in Pathogenic Mechanisms, Applications and Strategies for Entomopathogenic Fungi in Mosquito Biocontrol

Recent Advancements in Pathogenic Mechanisms, Applications and Strategies for Entomopathogenic Fungi in Mosquito Biocontrol

How Dopamine Influences Survival and Cellular Immune Response of Rhipicephalus microplus Inoculated with Metarhizium anisopliae

Extracts of Amazonian Fungi With Larvicidal Activities Against Aedes aegypti

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