Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) in elemental imaging of biological tissues and in proteomics

Becker, J. Sabine; Zoriy, Miroslav; Becker, J. Susanne; Dobrowolska, Justina; Matusch, Andreas

doi:10.1039/b701558e

Cited by 132 publications

(91 citation statements)

References 53 publications

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“…lA-icP-MS is a technique determining the ion release in the solid materials reliably and in a short time (31). lA-icP-MS also gives the opportunity for multi-element analysis of solid materials and is being used effectively in many fields (4,10). With LA-ICP-MS, along with food, marine speciation, solids and sediments, archaeological findings, botanical tissues, and environmental materials, it is now possible to directly examine biological samples such as teeth, hair and nails as well.…”

Section: Resultsmentioning

confidence: 99%

Titanium Element Level in Peri-Implant Mucosa

Mercan

Yayla

2013

Biotechnology & Biotechnological Equipment

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Section: Resultsmentioning

confidence: 99%

Titanium Element Level in Peri-Implant Mucosa

Mercan

Yayla

2013

Biotechnology & Biotechnological Equipment

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“…Soft targets such as slurries and gels present a problem because the impinging laser energy pulse tends to spatter the sample, which results in diminished signal pulses and leads to impractical analysis. This problem has been remedied by hardening the soft sample by immersing in liquid nitrogen for a short spell and quickly transferring the solidified target to the laser chamber for irradiation [27][28][29] . The integrity of the solidified sample can be easily monitored by the absence of splashing and the appearance of a crater akin to those that appear in actual solid targets.…”

Section: Solids and Soft Targetsmentioning

confidence: 99%

Deep-UV Ablative Laser Technology: A Technical Review

Pillay¹,

Samuel²,

Elkadi³

et al. 2017

Res. Rev. J Mat. Sci

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Ablative laser technology has been used successfully as a tool in scientific applications particularly to evaluate the homogeneity of materials and to depth-profile samples with the object of attaining elemental distribution at subsurface levels. Pulsed micro-beams strike a target with pinpoint accuracy and produce trace elemental information both spatially and in the substrate. Exploring different strata of a sample can produce data on impurities buried deep within the sample matrix. This is particularly important in cases where hidden impurities can make a difference to the performance of certain samples, such as semi-conductors or biomedical specimens. Stochastic effects such as imperfect crater formation, erratic energy pulses and unpredictable drift in beam energy could significantly affect the results of research applications. These technical features are controlled by sophisticated software, which plays a salient role in stabilizing the instrument. Samples are usually heterogeneous in nature, such as rocks, reservoir cores and concrete structures, and sample heterogeneity, therefore, is a factor that precludes adoption of conventional protocol for standardization of the technique. Soft samples such as gels and waxes could undergo standardization under special conditions. However, the technique is largely semi-quantitative for solids and is particularly attractive for exploring the homogeneity of solid targets, which reflects the level of elemental distribution within the sample matrix. The laser unit is coupled to a Perkin Elmer ICP-MS instrument and maintenance of consistent operation parameters is crucial for accurate and reproducible results. The laser beam wavelength is in the deep UV region and the system is operated with a beam of 213 nm of variable diameter between 5-100 μm, gas flow of 0.8 L/min, energy pulse rate of 60 MHz, and beam energy between 30-60%. Compared to other current instrumental techniques, ablative laser technology is superior for depth-profiling and surface analysis.

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“…SIMS directly produces images with a lateral resolution in the low-micrometre range [7], but owing to the presence of huge matrix effects and the high formation rate of polyatomic ions, SIMS quantification is difficult, as compared with quantification by GD-MS and LA-ICP-MS. On the other hand, LA-ICP-MS experiments have shown that the spatial resolution of LA analysis might be brought down to the nanoscale [8][9][10], whereas SNMS is not sensitive enough for trace element determinations [11]. A critical comparison of analytical capabilities, with selected examples and trends of LA-ICP-MS, GD-MS and SIMS/SNMS, is presented here as they can be considered the most widespread and powerful inorganic MS techniques currently applied for the direct characterisation of solids at the nanoscale.…”

Section: Solid State Mass Spectrometry Techniquesmentioning

confidence: 99%

“…Quantitative imaging of elements by MS in biological tissues is a challenging task in analytical chemistry and is relevant in different areas of biological and medical research. Becker et al [3,9,43] have investigated two-dimensional imaging of trace elements (e.g. Cu, Zn, Pb, P and S) in thin tissue sections of human and rat brains by LA-ICP-SFMS to employ them as indicators to monitor health risks.…”

Section: Laser Ablation Inductively Coupled Plasma Mass Spectrometrymentioning

confidence: 99%

Inorganic mass spectrometry as a tool for characterisation at the nanoscale

et al. 2009

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Inorganic mass spectrometry techniques may offer great potential for the characterisation at the nanoscale, because they provide unique elemental information of great value for a better understanding of processes occurring at nanometre-length dimensions. Two main groups of techniques are reviewed: those allowing direct solid analysis with spatial resolution capabilities, i.e. lateral (imaging) and/or indepth profile, and those for the analysis of liquids containing colloids. In this context, the present capabilities of widespread elemental mass spectrometry techniques such as laser ablation coupled with inductively coupled plasma mass spectrometry (ICP-MS), glow discharge mass spectrometry and secondary ion/neutral mass spectrometry are described and compared through selected examples from various scientific fields. On the other hand, approaches for the characterisation (i.e. size, composition, presence of impurities, etc.) of colloidal solutions containing nanoparticles by the well-established ICP-MS technique are described. In this latter case, the capabilities derived from the on-line coupling of separation techniques such as field-flow fractionation and liquid chromatography with ICP-MS are also assessed. Finally, appealing trends using ICP-MS for bioassays with biomolecules labelled with nanoparticles are delineated.

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Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) in elemental imaging of biological tissues and in proteomics

Cited by 132 publications

References 53 publications

Titanium Element Level in Peri-Implant Mucosa

Titanium Element Level in Peri-Implant Mucosa

Deep-UV Ablative Laser Technology: A Technical Review

Inorganic mass spectrometry as a tool for characterisation at the nanoscale

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