Laser and light-emitting diode effects on pre-osteoblast growth and differentiation

Pagin, Marina T.; Oliveira, Flávia Amadeu de; Oliveira, Rodrigo Cardoso de; Sant’Ana, Adriana Campos Passanezi; Rezende, Maria Lúcia Rubo de; Greghi, Sebastião Luiz Aguiar; Damante, Carla Andreotti

doi:10.1007/s10103-012-1238-5

Cited by 56 publications

(36 citation statements)

References 29 publications

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“…The wavelength of such lasers is reported to be 500–1200 nm. Recent literatures regarding pre-osteoblast stimulation with red laser were reported [28–31]. We used a Ga–As–Al laser with a wavelength of 808 nm, which is within the prescribed range.…”

Section: Discussionmentioning

confidence: 99%

Effect of low-level laser therapy on bisphosphonate-treated osteoblasts

Shin

Kim

Choi

et al. 2016

Maxillofac Plast Reconstr Surg

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BackgroundThis study investigates the effect of alendronate-treated osteoblasts, as well as the effect of low-level laser therapy (LLLT) on the alendronate-treated osteoblasts. Bisphosphonate decreases the osteoblastic activity. Various treatment modalities are used to enhance the bisphosphonate-treated osteoblasts; however, there were no cell culture studies conducted using a low-level laser.MethodsHuman fetal osteoblastic (hFOB 1.19) cells were treated with 50 μM alendronate. Then, they were irradiated with a 1.2 J/cm2 low-level Ga-Al-As laser (λ = 808 ± 3 nm, 80 mW, and 80 mA; spot size, 1 cm2; NDLux, Seoul, Korea). The cell survivability was measured with the MTT assay. The three cytokines of osteoblasts, receptor activator of nuclear factor κB ligand (RANKL), osteoprotegerin (OPG), and macrophage colony-stimulating factor (M-CSF) were analyzed.ResultsIn the cells treated with alendronate at concentrations of 50 μM and higher, cell survivability significantly decreased after 48 h (p < 0.05). After the applications of low-level laser on alendronate-treated cells, cell survivability significantly increased at 72 h (p < 0.05). The expressions of OPG, RANKL, and M-CSF have decreased via the alendronate. The RANKL and M-CSF expressions have increased, but the OPG was not significantly affected by the LLLT.ConclusionsThe LLLT does not affect the OPG expression in the hFOB cell line, but it may increase the RANKL and M-CSF expressions, thereby resulting in positive effects on osteoclastogenesis and bone remodeling.

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Section: Discussionmentioning

confidence: 99%

Effect of low-level laser therapy on bisphosphonate-treated osteoblasts

Shin

Kim

Choi

et al. 2016

Maxillofac Plast Reconstr Surg

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“…Low-power laser irradiation promotes the proliferation and differentiation of osteogenic cell lines, such as MC3T3-E1 cells13. Although lasers emit coherent light and LEDs emit non-coherent light, both induce similar photobiological effects when used with the same irradiation parameters5.…”

Section: Discussionmentioning

confidence: 99%

Effects of light-emitting diode irradiation on the osteogenesis of human umbilical cord mesenchymal stem cells in vitro

Yang

Wang

et al. 2016

Sci Rep

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The aim of this study was to examine the effects of light-emitting diode (LED) photobiomodulation therapy on the proliferation and differentiation of human umbilical cord mesenchymal stem cells (hUMSCs) cultured in osteogenic differentiation medium. HUMSCs were irradiated with an LED light at 620 nm and 2 J/cm2 and monitored for cell proliferation and osteogenic differentiation activity. The experiment involved four groups of cells: the control group; the osteogenic group (osteo group); the LED group; the osteogenic + LED group (LED + osteo group). HUMSC proliferation was detected by performing a3-(4,5-dimethylthiazol-2yl)-2,5 diphenyltetrazolium bromide(MTT) assay. Osteogenic activity was evaluated by performing alkaline phosphatase (ALP) and Von Kossa staining, and osteopontin (OPN) gene mRNA expression was evaluated byreverse transcription polymerase chain reaction (RT-PCR). The hUMSCs in the LED + osteo group exhibited a significantly higher proliferation rate than the other subgroups. Additionally, there were greater numbers of ALP-positive cells and Von Kossa nodules in the LED + osteo group. OPN mRNA expression in the LED + osteo group was higher than other subgroups. In conclusion, low levels of LED light at a wavelength of 620 nm enhance the proliferation and osteogenic differentiation of hUMSCs during a long culture period.

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“…Faced with a heterogeneous set of experimental studies based on a variety of animal models, it is not possible to extrapolate the results to human subjects. [50][51][52][53][54][55][56][57] The majority of human studies demonstrate a positive benefit and it could be considered that the treatment is seen as passing the threshold as an evidenced-based approach. Given the heterogeneous wavelengths and parameters employed in the limited number of studies accepted for inclusion, it is premature to offer a protocol to guide clinicians.…”

Section: Pbmt: Accelerated Otmmentioning

confidence: 99%

Systematic Review of Orthodontic Treatment Management with Photobiomodulation Therapy

Cronshaw

Parker

Anagnostaki

et al. 2019

Photobiomodulation, Photomedicine, and Laser Surgery

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Objective: This systematic review of published research evaluates the parameters and reported outcomes in photobiomodulation therapies (PBMTs) used in management of pain and accelerated tooth movement during orthodontic treatment. Background: Consequent to positive in vitro and animal studies, there has been considerable interest in the potential applications of PBMT to ameliorate pain and to accelerate orthodontic tooth movement. Due to the lack of consistency of wavelengths and parameters applied, there is as yet no consensus in respect of guidance for clinical use. Within the limitations of the reported literature, a digest of key outcomes and parameters is presented here to assist researchers and interested clinicians. Materials and methods: Search engines, PubMed, Google Scholar, and Cochrane, were applied to identify clinical investigations into the potential benefits of PBMTs in orthodontic management. Keywords used were ''low-level laser therapy,'' ''low-level light therapy,'' ''photobiomodulation,'' ''orthodontics,'' ''pain,'' ''tooth movement,'' and ''randomized clinical trial ‡5 years.'' A total of 35 articles were identified, covering the 5-year period January 2013 to December 2018. Only human clinical trials were considered. Seventeen articles were included in this investigation and the reported parameters and outcomes were assessed and presented for comparison. Results: Seven of nine studies supported the effectiveness of PBMT to reduce or prevent pain. There was no consensus on choice of wavelength and a range of parameters were found beneficial. Of the seven tooth movement studies included, four of the seven were positive, of which two used a home-use light-emitting diode (LED) device, as opposed to a clinician-applied laser. A variety of wavelengths and parameters were found to be effective in accelerating tooth movement in the range of 20-40% compared with control. Conclusions: Due to a lack of consistency in approach, further studies are required to achieve a high level of acceptance. The clinical logistics associated with frequent applications supports the concept of patient home delivery use of LED devices, although there are insufficient studies at present to make an evidence-based determination of this type of appliance. To gain more general professional acceptance of the use of PBMT in orthodontics, a continued effort to extend the number of quality human clinical trials is required. Based on the current evidence base, further positive onward progression can be regarded as inevitable.

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Laser and light-emitting diode effects on pre-osteoblast growth and differentiation

Cited by 56 publications

References 29 publications

Effect of low-level laser therapy on bisphosphonate-treated osteoblasts

Effect of low-level laser therapy on bisphosphonate-treated osteoblasts

Effects of light-emitting diode irradiation on the osteogenesis of human umbilical cord mesenchymal stem cells in vitro

Systematic Review of Orthodontic Treatment Management with Photobiomodulation Therapy

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