Laser-assisted ICSI: a novel approach to obtain higher oocyte survival and embryo quality rates

Abdelmassih, S.; Cardoso, J. de N. O.; Abdelmassih, V.; Dias, Júlia A; Abdelmassih, Roger; Nagy, Zsolt

doi:10.1093/humrep/17.10.2694

Cited by 77 publications

(37 citation statements)

References 39 publications

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“…Laser technology is a simple, efficient, and precise cellular microsurgery [35,36] tool that has been used in ART in recent years. In this study, a short duration laser pulse was directed once at the junction between the two trophectoderm cells in a region away from the ICM to reduce the blastocoelic fluid before vitrification.…”

Section: Discussionmentioning

confidence: 99%

Artificial shrinkage of blastocoel using a laser pulse prior to vitrification improves clinical outcome

Darwish¹,

Magdi²

2016

J Assist Reprod Genet

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Purpose Blastocysts contain a large amount of fluid in the blastocoel, which may pose a risk for ice crystal formation during vitrification. This study aimed to evaluate the effectiveness of laser-induced artificial shrinkage of blastocoel before vitrification on clinical outcome. Methods Patients were divided into two groups: a control group with untreated, expanded blastocysts (n = 115) and a study group with blastocoel artificially eliminated by a laser pulse prior to vitrification (n = 309). Blastocyst survival, clinical pregnancy, and implantation rates were compared. Result(s) The survival rate was significantly higher in the study group compared with the control group (97.3 and 74.9 %, respectively; p > 0.01). The clinical pregnancy and implantation rates of the study group were significantly higher (p < 0.01) than that of the control group (clinical pregnancy, 67.2 vs. 41.1 %; implantation, 39.1 vs. 24.5 %. Conclusion(s) This study demonstrated that the removal of blastocoel fluid before vitrification by laser pulse of in vitroproduced human blastocysts significantly improves blastocyst survival, clinical pregnancy, and implantation rates.

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Section: Discussionmentioning

confidence: 99%

Artificial shrinkage of blastocoel using a laser pulse prior to vitrification improves clinical outcome

Darwish¹,

Magdi²

2016

J Assist Reprod Genet

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“…This framentation is associated with significantly poorer embryo quality [3,6,8] and a reduced rate of compaction as compared to cycles without fragile ZPs [49]. Consequently, such embryos may not have the ability to implant and are subsequently excluded from the treatment process [19].…”

Section: Discussionmentioning

confidence: 99%

“…In order to avoid these inadvertent consequences, several assisted ICSI/hatching techniques such as Piezoassisted ICSI (penetration of the ZP alone with a piezopulse to perform ICSI) [15,16] and laser-assisted ICSI (drilling a microhole through the ZP or thinning the ZP with a laser beam immediately prior to ICSI) [17][18][19][20] have been suggested.…”

Section: Introductionmentioning

confidence: 99%

Estimating zona pellucida hardness under microinjection to assess oocyte/embryo quality: Analytical and experimental studies

Khalilian¹,

Valojerdi²,

Navidbakhsh³

et al. 2013

ABB

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The precise determination of zona pellucida (ZP) hardness is largely unknown due to the lack of appropriate measuring and modelling methods. In this study, we have used experimental and theoretical models to describe the mechanical behavior of a single oocyte cell to improve the assisted reproductive technology (ART) outcomes by assessing oocyte/embryo quality. This paper presents the development of: i) a microinjection model to estimate the force of ZP penetration, ii) a micropipette aspiration model to determine the corresponding hardness, and iii) an experimental procedure to generate the required data for these two models. Our results show that the estimated penetration force provides a performance target for the penetration process during intracytoplasmic sperm injection (ICSI), while the estimated corresponding hardness serves as an indicator of the extent of deformation sustained by the oocyte prior to penetration. Evaluation of these results shows that a routine assessment of ZP hardness under microinjection would allow for the identification of certain oocyte pools for which further manipulation is recommended in order to improve injection, hatching and finally ART outcomes.

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“…Administration of hCG (Ovitrelle, 250 mcg; Serono) occurred when follicular size and E2 levels were appropriate and oocyte retrieval was performed by ultrasound-guided puncture under general anesthesia 36 hours later. The all metaphase II (M II) oocytes obtained were fertilized by standard intracytoplasmic sperm injection (ICSI), and embryo transfer was carried out on Day 3 or Day 5 after oocyte retrieval [13]. Transvaginal progesterone (Progestan 200 mg Kocak) was initiated from the day of the oocyte pick-up and continued until the assessment of pregnancy (12 days after ET) to support the luteal phase.…”

Section: Methodsmentioning

confidence: 99%

Does Placement of the Catheter Guide Through the Cervical Canal Influence the Embryo Transfer Outcome?

Bıldırcın¹

2016

Ann Clin Anal Med

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ETs were carried out in women undergoing IVF treatment. Although the pregnancy ratio seemed to be higher among the transfers carried out with the inner catheter alone inserted through the cervix, no statistically significance for this was detected (odds ratio: 1.644, 95% confidence bound, p>0.05).Discussion: Placement of the catheter guide through the cervical canal at the time of embryo expulsion may not have any effect on the IVF-ET outcome.

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Laser-assisted ICSI: a novel approach to obtain higher oocyte survival and embryo quality rates

Abstract: Creating a microhole on the zona pellucida of the oocyte by laser beam prior to ICSI provides a less traumatic penetration of the injection needle into the ooplasm and results in lower degeneration and higher embryo development rates than C-ICSI in patients with fragile oocytes.

Cited by 77 publications

References 39 publications

Artificial shrinkage of blastocoel using a laser pulse prior to vitrification improves clinical outcome

Artificial shrinkage of blastocoel using a laser pulse prior to vitrification improves clinical outcome

Estimating zona pellucida hardness under microinjection to assess oocyte/embryo quality: Analytical and experimental studies

Does Placement of the Catheter Guide Through the Cervical Canal Influence the Embryo Transfer Outcome?

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