Laser Engineered Graphene Paper for Mass Spectrometry Imaging

Qian, Kun; Zhou, Liang; Liu, Jian; Yang, Jie; Xu, Hongyi; Yu, Meihua; Nouwens, Amanda; Zou, Jin; Monteiro, Michael J.; Yu, Chengzhong

doi:10.1038/srep01415

Cited by 46 publications

(45 citation statements)

References 31 publications

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“…Previously we have reported particle assisted laser desorption/ionization mass spectrometry (PALDI MS) for small metabolites analysis from biological samples [4a, 15] and immunomagnetic assays for efficient CTCs isolation in blood. [2a, 2b, 16] Herein, we demonstrated the combination of rare cell isolation and PALDI MS to detect the metabolism of captured CTCs for metabolic study based on functional ferric oxide magnetic particles (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

Multifunctional Magnetic Particles for Combined Circulating Tumor Cells Isolation and Cellular Metabolism Detection

Wei

Gan

et al. 2016

Adv Funct Materials

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102

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We for the first time demonstrate multi-functional magnetic particles based rare cell isolation combined with the downstream laser desorption/ionization mass spectrometry (LDI-MS) to measure the metabolism of enriched circulating tumor cells (CTCs). The characterization of CTCs metabolism plays a significant role in understanding the tumor microenvironment, through exploring the diverse cellular process. However, characterizing cell metabolism is still challenging due to the low detection sensitivity, high sample complexity, and tedious preparation procedures, particularly for rare cells analysis in clinical study. Here we conjugate ferric oxide magnetic particles with anti-EpCAM on the surface for specific, efficient enrichment of CTCs from PBS and whole blood with cells concentration of 6–100 cells per mL. Moreover, these hydrophilic particles as matrix enable sensitive and selective LDI-MS detection of small metabolites (MW<500 Da) in complex bio-mixtures and can be further coupled with isotopic quantification to monitor selected molecules metabolism of ~50 CTCs. Our unique approach couples the immunomagnetic separation of CTCs and LDI-MS based metabolic analysis, which represents a key step forward for downstream metabolites analysis of rare cells to investigate the biological features of CTCs and their cellular responses in both pathological and physiological phenomena.

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Section: Introductionmentioning

confidence: 99%

Multifunctional Magnetic Particles for Combined Circulating Tumor Cells Isolation and Cellular Metabolism Detection

Wei

Gan

et al. 2016

Adv Funct Materials

Self Cite

102

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“…This technique increases the stability of the graphene paper as well as its hydrophobicity and electric conductivity. It also increases the imaging of trace element of small molecules and the detection limit is also increased twofold compared to other commercial products [155]. As a very elemental form of graphene, 2D graphene has high compatibility and duration needed for many imaging mass spectrometry (IMS) experiments.…”

Section: Graphene In Mass Spectrometrymentioning

confidence: 99%

Graphene and graphene oxide as nanomaterials for medicine and biology application

et al. 2018

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Graphene-and graphene oxide-based nanomaterials have gained broad interests in research because of their unique physiochemical properties. The 2D allotropic structure allows it to be used in various biological fields. The biomedical applications of graphene and its composite include its use in gene and small molecular drug delivery. It is further used for biofunctionalization of protein, in anticancer therapy, as an antimicrobial agent for bone and teeth implantation. The biocompatibility of the newly synthesized nanomaterials allows its substantial use in medicine and biology. The current review summarizes the chemical structure and biological application of graphene in various fields.

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“…MSI has yet to be performed on tissue samples utilizing ice as the matrix. Another matrix-free imaging strategy was developed by Qian et al [136]. In this research, a graphene paper was engineered using a pulse-laser process to form densely packed nanospheres.…”

Section: Challenges Of Neurotransmitter Msimentioning

confidence: 99%

Challenges and Recent Advances in Mass Spectrometric Imaging of Neurotransmitters

Gemperline

Chen

2014

Bioanalysis

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Mass spectrometric imaging (MSI) is a powerful tool that grants the ability to investigate a broad mass range of molecules, from small molecules to large proteins, by creating detailed distribution maps of selected compounds. To date, MSI has demonstrated its versatility in the study of neurotransmitters and neuropeptides of different classes toward investigation of neurobiological functions and diseases. These studies have provided significant insight in neurobiology over the years and current technical advances are facilitating further improvements in this field. neurotransmitters, focusing specifically on the challenges and recent Herein, we advances of MSI of neurotransmitters.

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Laser Engineered Graphene Paper for Mass Spectrometry Imaging

Cited by 46 publications

References 31 publications

Multifunctional Magnetic Particles for Combined Circulating Tumor Cells Isolation and Cellular Metabolism Detection

Multifunctional Magnetic Particles for Combined Circulating Tumor Cells Isolation and Cellular Metabolism Detection

Graphene and graphene oxide as nanomaterials for medicine and biology application

Challenges and Recent Advances in Mass Spectrometric Imaging of Neurotransmitters

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