Laser-induced fluorescence in malignant and normal tissue in mice injected with two different carotenoporphyrins

Nilsson, Henrik; Johansson, Jonas; Svanberg, Katarina; Svanberg, Sune; Jori, G; Reddi, Elena; Segalla, Anna; Gust, Devens; Moore, Ana L.; Moore, Thomas A.

doi:10.1038/bjc.1994.413

Cited by 24 publications

(17 citation statements)

References 25 publications

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“…It has previously been shown that the recorded CP fluorescence from tumour exterior increases over the first 48 h, and then drops during the consecutive 48 h, to reach a low level at 96 h after injection (Nilsson et al, 1994;Reddi et al, 1994). It can be concluded from Figure 3 that no organ except the liver exhibits a stronger CP(Me)3 fluorescence at 654 nm than does the tumour exterior.…”

Section: Materials and Methods Chemicalsmentioning

confidence: 72%

“…The interaction of haemoglobin was particularly prominent when monitoring the lungs, spleen and trachea. This was also evident in the necrotic tumours, where haemorrhages occurred when measurements were made of the interior part of the tumours (Nilsson et al, 1994). Sometimes this interference was evident on probing the exterior capsule of the tumour, because of subcapsular haemorrhages, again due to tumour necrosis.…”

Section: Materials and Methods Chemicalsmentioning

confidence: 92%

“…This fact holds true for both CPs. The tumour to normal surrounding muscle ratio has previously been found to range from 8:1 to 10:1 for CP(Me)3 and from 9:1 to 12:1 for CP(OMe)3, when including the autofluorescence in the discrimination criterion (Nilsson et al, 1994). Figures 3,4,7 and 8 show that the substance-related fluorescence was higher in the measurements of the empty bladders than of the full ones, whereas the endogenous fluorescence was much dominant in the case of the full bladders.…”

Section: Discussionmentioning

confidence: 99%

“…The interference from non-substance-related peaks, described for Figure 3, is not as prominent in Figure 4, probably because of a higher accumulation of the trimethoxylated carotenoporphyrin or because of a higher fluorescence yield, which allows the substance-related fluorescence to dominate over other fluorescence signatures. In the case of CP(OMe)3, tumour exterior exhibits its British Journal of Cancer (1997) strongest 658 nm fluorescence 3 h after administration of the substance and then the intensity slowly decreases over time (Nilsson et al, 1994). As in the case of CP(Me)3, the liver is the only organ with a substance-related fluorescence similar to that of tumour exterior.…”

Section: Materials and Methods Chemicalsmentioning

confidence: 98%

“…The y-axes display the fluorescence intensities at 654 nm (A) for the various tissues, expressed in units relative to tumour exterior (i.e. measurements on the tumours after removal of the overlying skin ;Nilsson et al, 1994). The value of tumour exterior is set to unity.…”

Section: Materials and Methods Chemicalsmentioning

confidence: 99%

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Laser-induced fluorescence studies of the biodistribution of carotenoporphyrins in mice

Nilsson

Johansson²,

Svanberg³

et al. 1997

Br J Cancer

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Summary The biodistribution of two recently developed tumour markers, trimethylated (CP(Me)3) and trimethoxylated (CP(OMe)3) carotenoporphyrin, was investigated by means of laser-induced fluorescence (LIF) after i.v. injection into 38 tumour-bearing (MS-2 fibrosarcoma) female Balb/c mice. At 3, 24, 48 or 96 h after administration, the carotenoporphyrin fluorescence was measured in tumoral and peritumoral tissue, as well as in the abdominal, thoracic and cranial cavities. The fluorescence was induced by a nitrogen laser-pumped dye laser, emitting light at 425 nm, and analysed by a polychromator equipped with an image-intensified CCD camera. The fluorescence was evaluated at 490, 655 and 720 nm: the second and third wavelengths represent the carotenoporphyrin (CP)-related peaks, whereas the first one is close to the peak of the tissue autofluorescence. The tumour and the liver were the two tissue types showing the strongest carotenoporphyrin-related fluorescence, whereas the cerebral cortex and muscle consistently exhibited weak substance-related fluorescence. In most tissue types, the fluorescence intensities decreased over time. A few exceptions were observed, notably the liver, in which the intensity remained remarkably constant over the time period investigated.

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Section: Materials and Methods Chemicalsmentioning

confidence: 72%

Section: Materials and Methods Chemicalsmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Materials and Methods Chemicalsmentioning

confidence: 98%

Section: Materials and Methods Chemicalsmentioning

confidence: 99%

See 3 more Smart Citations

Laser-induced fluorescence studies of the biodistribution of carotenoporphyrins in mice

Nilsson

Johansson²,

Svanberg³

et al. 1997

Br J Cancer

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Laser-induced fluorescence studies of normal and malignant tumour tissue of rat following intravenous injection of δ-amino levulinic acid

Johansson¹,

Berg²,

Svanberg³

et al. 1997

Lasers Surg. Med.

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Synthesis of meso-nitrophenylporphyrins covalently linked to a polyphenylene chain bearing methoxy groups

Durantini

2000

J. Porphyrins Phthalocyanines

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A convenient procedure for the synthesis of polyphenylene porphyrin dyads is described. The dyads consist of a meso-nitrophenylporphyrin covalently linked to a polyphenylene chain by an amide bond. The final phenyl group in the chain bears electron donor methoxy groups. The π-conjugated chain was obtained in excellent yield via a Wittig–Horner reaction, which produces a new double bond (E isomer) incorporating either — COOCH 3 or E isomer) incorporating either — CON ( OCH 3)( CH 3) functional groups. Expansion of the conjugated chain involves the reduction of these groups to aldehydes followed by a second Wittig–Horner reaction. 5-(4-Aminophenyl)-10,15,20-tris(4-nitrophenyl)porphyrin was synthesized from meso-(4-nitrophenyl)dipyrromethane. The E isomer) incorporating either — NO 2 groups in para positions of the peripheral phenyl rings give extra electron-withdrawing character to the porphyrin macrocycles. Coupling of the polyphenylene acids with aminoporphyrin results in the desired dyads. These dyads have moieties with different electron donor–acceptor properties. This present strategy may be easily used for preparation of other similar dyad derivatives. These compounds can be suitable for photodynamic therapy.

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Laser-induced fluorescence in malignant and normal tissue in mice injected with two different carotenoporphyrins

Cited by 24 publications

References 25 publications

Laser-induced fluorescence studies of the biodistribution of carotenoporphyrins in mice

Laser-induced fluorescence studies of the biodistribution of carotenoporphyrins in mice

Laser-induced fluorescence studies of normal and malignant tumour tissue of rat following intravenous injection of δ-amino levulinic acid

Synthesis of meso-nitrophenylporphyrins covalently linked to a polyphenylene chain bearing methoxy groups

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