Laser induced fluorescence spectroscopy from native cancerous and normal tissue

Alfano, R. R.; Tata, Darrell B.; Cordero, José Manuel Gonzalo; Tomashefsky, Philip; Longo, F.; Alfano, Marco

doi:10.1109/jqe.1984.1072322

Cited by 443 publications

(202 citation statements)

References 6 publications

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“…Some of the earliest work on diagnostic fluorescence spectroscopy, by Profio et al 10 and by Alfano et al 11 addressed differences in the native UV-induced fluorescence in tissues of different pathological states. Examples of translational research of fluorescence spectroscopy for cancer detection in vivo include studies for cervical cancer, [12][13][14] lung cancer, 15 colon cancer, 16 and oral neoplasia.…”

Section: Fluorescence Spectroscopymentioning

confidence: 99%

Spectrroscopic Sensing of Cancer and Cancer Therapy: Current Status of Translational Research

Bigio

Bown²

2004

Cancer Biology & Therapy

144

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Various types of optical spectroscopy have been investigated as methods to effect a non-invasive, real-time in-situ assessment of tissue pathology. All of these methods have one basic principle in common: the optical spectrum of a tissue contains information about the biochemical composition and/or the structure of the tissue, and that information conveys diagnostic information. The biochemical information can be obtained by measuring absorption, fluorescence, or Raman scattering signals. Structural and morphological information may be obtained by techniques that assess the elastic-scattering properties of tissue. These basic approaches are useful for the detection of cancer as well as for other diagnostic applications such as hemoglobin saturation, intra-luminal detection of atherosclerosis, and simply the identification of different tissue types during procedures. Optical spectroscopic measurements can also be employed in the management of disease treatment. The site-specific pharmacokinetics of chemotherapy and photodynamic therapy agents can be used to customize dosage to the patient, and diagnostic spectroscopy can be used to monitor response to treatment. In recent years clinical studies have provided indications of potential efficacy, and some of these modalities are now entering a translational research stage, with an eye to approval and commercialization. A benefit of these methods is their inherent low cost and ease of implementation, generally mediated with small portable instruments, not requiring any specialized facilities, and eventually not requiring expert interpretation. This paper reviews briefly the most common methods of diagnostic optical spectroscopy, and reviews in greater depth recent clinical translational research invoking scattering spectroscopy as the enabling technology, which has been the experience of the authors.

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Section: Fluorescence Spectroscopymentioning

confidence: 99%

Spectrroscopic Sensing of Cancer and Cancer Therapy: Current Status of Translational Research

Bigio

Bown²

2004

Cancer Biology & Therapy

144

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“…[52,53] The fluorescence spectra were obtained with an argon laser beam at 488 nm and power of up to 100 mW focused on a spot of about 0.1 mm in diameter. These fluorescence spectra were shown to be dominated by a broadly peaked curve in the wavelength range from 500 to 650 nm with no significant structure.…”

Section: Spectroscopy Measurementsmentioning

confidence: 99%

Optical Detection of Cancers

Hu¹,

Lu²

2008

Encyclopedia of Biomaterials and Biomedical Engineering, Second Edition - Four Volume Set

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“…Pioneering work by Alfano et al highlighted the potential of autofluorescence spectroscopy for cancer detection 20 . In a paper by Liu et al 21 , Alfano and coworkers explored the use of autofluorescence, Raman scattering and time-resolved light scattering approaches as optical diagnostic techniques to separate diseased and normal breast tissues.…”

Section: Autofluorescence Spectroscopymentioning

confidence: 99%

Advances in Optical Spectroscopy and Imaging of Breast Lesions

Demos

Vogel

Gandjbakhche

2006

J Mammary Gland Biol Neoplasia

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Abstract:A review is presented of recent advances in optical imaging and spectroscopy and the use of light for addressing breast cancer issues. Spectroscopic techniques offer the means to characterize tissue components and obtain functional information in real time. Three-dimentional optical imaging of the breast using various illumination and signal collection schemes in combination with image reconstruction algorithms may provide a new tool for cancer detection and monitoring of treatment. 2 Chapter 1. BackgroundAccording to the National Institutes of Health, breast cancer is the most diagnosed nonskin cancer and the second leading cause of cancer death among women in the United States.Although the breast cancer diagnosis rate has increased, there has been a steady drop in the overall breast cancer death rate since the early 1990s. Depending on the size of the tumor and the involvement of the lymph nodes, survival rates can vary from 45-95%. The key problem with the breast is the variation in demographic size, texture, chemical composition, and age. X-ray mammography is the gold standard for breast cancer screening and detection. Mammography is most sensitive in women over 35-40 years of age because of their fatty breast composition 1 and less effective and sensitive in younger women because they have denser breasts 2 . For younger women, ultrasound is commonly used. Magnetic resonance imaging (MRI) can also play a significant role in the diagnosis and characterization of breast disease.According to the National Cancer Society, up to 10% of all breast cancers, roughly 20,000 cases per year in the U.S., are not discovered by X-ray mammography. Also, X-ray mammography uses ionizing radiation and requires uncomfortable breast compression. It also suffers from a significant number of false positives that often lead to unnecessary biopsy since biopsy is generally required to determine malignancy in most women with an abnormal Spatial and temporal contrasts in these properties may be uniquely useful for diagnosing disease.The objective of this work is to provide a comprehensive review of the progress of photonics methods to address breast cancer issues. This review is divided into three major focus areas: a) optical spectroscopy methods suitable for tissue evaluation in real time; b) noninvasive NIR spectroscopy for the acquisition of functional information; and c) optical imaging methods for functional tumor characterization. Chapter 2. Optical biopsy methodsThe term "optical biopsy" describes the use of optical spectroscopy to characterize tissue, and requires direct exposure of the tissue under examination to the light source. Consequently, its application in a clinical setting is best suited for intraoperative use to assist in exploring tissue in real time, or via thin fiberoptic needles that can reach the suspected location within the breast for a minimally invasive evaluation.The breast is complex; its multiple tissue components make it more difficult to classify using optical spectroscopy than other tissues (...

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Laser induced fluorescence spectroscopy from native cancerous and normal tissue

Cited by 443 publications

References 6 publications

Spectrroscopic Sensing of Cancer and Cancer Therapy: Current Status of Translational Research

Spectrroscopic Sensing of Cancer and Cancer Therapy: Current Status of Translational Research

Optical Detection of Cancers

Advances in Optical Spectroscopy and Imaging of Breast Lesions

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