Laser-induced topographies enable the spatial patterning of co-cultured peripheral nervous system cells

Angelaki, Despoina; Kavatzikidou, Paraskevi; Fotakis, C.; Stratakis, Emmanuel; Ranella, Anthi

doi:10.1016/j.msec.2020.111144

Cited by 11 publications

(16 citation statements)

References 46 publications

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“…These findings are in good agreement with previous work in our lab [24]. More specifically, Angelaki et al have designed patterned micro-sized spikes and nano-sized ripples for the co-culture of SW10/N2a cells.…”

Section: Discussionsupporting

confidence: 92%

“…In Figure 4, it can be seen that when co-culturing both cell types on glass coverslips, a similar behavior to what has previously been reported [24] is observable. More specifically, SW10 cells tend to attach to the glass surface, while N2a cells either attach to the glass (if there is empty space) or lay on top of the SW10 cell layer.…”

Section: Sw10/n2a Co-culturessupporting

confidence: 83%

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Development of an Oriented Co-Culture System Using 3D Scaffolds Fabricated via Non-Linear Lithography

et al. 2022

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Damage in the Peripheral Nervous System (PNS) is related to numerous neurodegenerative diseases and has consequently drawn the attention of Tissue Engineering (TE), which is considered a promising alternative to already established methods such as surgery and autografts. TE focuses on the design, optimization, and use of scaffolds in vitro and in vivo. In this work, the authors used a novel scaffold geometry fabricated via Multiphoton Lithography (MPL), a commonly used fabrication method, for the mono- and co-cultures of glial Schwann (SW10) and neuronal Neuro-2a (N2a) cells. Both cell types have already been used for the study of various neurodegenerative diseases. However, their focus has been on only one of the cell types at a time, with studies regarding their co-culture only recently documented. Here, the suitability of the fabricated scaffolds has been explored and the effects of topography on SW10 and N2a behavior have been investigated. Our findings demonstrate that scaffold co-culture systems favor the presence of neurites compared to mono-cultures at 21 days (31.4 ± 5.5% and 15.4 ± 5.4%, respectively), while there is also a significant decrease in long neurites in the mono-culture over time (45.3 ± 15.9% at 7 days versus 15.4 ± 5.4% at 21 days). It has been shown that the scaffolds can successfully manipulate cell growth, elongation, and morphology, and these results can form a basis for the development of an experimental model for the study of PNS-related diseases and understanding of key cell functions such as myelination.

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Section: Discussionsupporting

confidence: 92%

Section: Sw10/n2a Co-culturessupporting

confidence: 83%

Development of an Oriented Co-Culture System Using 3D Scaffolds Fabricated via Non-Linear Lithography

et al. 2022

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“…Various topographical features have been investigated in literature (Lotfi et al, 2019 ). These features include but are not limited to, micro- and nano-scale surface topography and patterning (Miller et al, 2001 ; Schmalenberg and Uhrich, 2005 ; Li et al, 2014 ; Yiannakou et al, 2017 ; Angelaki et al, 2020 ), cell imprinted topography (Moosazadeh Moghaddam et al, 2019 ), scaffold geometry (grooves, filaments, wells, pillars etc. ; Schmalenberg and Uhrich, 2005 ; Chew et al, 2008 ; Mitchel and Hoffman-Kim, 2011 ; Mobasseri et al, 2014 ; Tonazzini et al, 2015 ; Chen et al, 2019 ), hydrophilicity (Mobasseri et al, 2014 ), and roughness (Simitzi et al, 2015 ; Babaliari et al, 2021 ; Huang et al, 2021 ) and porosity (Li et al, 2014 ).…”

Section: The Effect Of the Microenvironment On Schwann Cell Behaviourmentioning

confidence: 99%

“…Another point of interest pertains to studies that have focused on co-culturing neurons alongside SCs since the nervous system is very complicated and multiple parameters should be taken into account for the successful application of scaffolds as in vivo transplants (Angelaki et al, 2020 ; Kordas et al, 2022 ). One such study utilised a substrate with combined morphology including both micro-cones and nano-ripples for the co-culture of SCs and neuronal cells.…”

Section: The Effect Of the Microenvironment On Schwann Cell Behaviourmentioning

confidence: 99%

“…One such study utilised a substrate with combined morphology including both micro-cones and nano-ripples for the co-culture of SCs and neuronal cells. The authors were able to show that SCs adhesion was affected by the underlying pattern, which in turn also influenced neuronal cell behaviour (Angelaki et al, 2020 ).…”

Section: The Effect Of the Microenvironment On Schwann Cell Behaviourmentioning

confidence: 99%

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The role of mechanobiology on the Schwann cell response: A tissue engineering perspective

Manganas

Kavatzikidou

Kordas

et al. 2022

Front. Cell. Neurosci.

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Schwann cells (SCs), the glial cells of the peripheral nervous system (PNS), do not only form myelin sheaths thereby insulating the electrical signal propagated by the axons, but also play an essential role in the regeneration of injured axons. SCs are inextricably connected with their extracellular environment and the mechanical stimuli that are received determine their response during development, myelination and injuries. To this end, the mechanobiological response of SCs is being actively researched, as it can determine the suitability of fabricated scaffolds for tissue engineering and regenerative medicine applications. There is growing evidence that SCs are sensitive to changes in the mechanical properties of the surrounding environment (such as the type of material, its elasticity and stiffness), different topographical features provided by the environment, as well as shear stress. In this review, we explore how different mechanical stimuli affect SC behaviour and highlight the importance of exploring many different avenues when designing scaffolds for the repair of PNS injuries.

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Analysis of contributions of electron-phonon relaxation and recombination processes to silicon heating by femtosecond laser pulse

2022

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Laser-induced topographies enable the spatial patterning of co-cultured peripheral nervous system cells

Cited by 11 publications

References 46 publications

Development of an Oriented Co-Culture System Using 3D Scaffolds Fabricated via Non-Linear Lithography

Development of an Oriented Co-Culture System Using 3D Scaffolds Fabricated via Non-Linear Lithography

The role of mechanobiology on the Schwann cell response: A tissue engineering perspective

Analysis of contributions of electron-phonon relaxation and recombination processes to silicon heating by femtosecond laser pulse

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