Laser Interference Microscopy for Nanobiotechnologies

Ignatyev, Pavel S.; Indukaev, K. V.; Osipov, P. A.; Sergeev, I. K.

doi:10.1007/s10527-013-9328-7

Cited by 9 publications

(5 citation statements)

References 10 publications

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“…According to TEM data with elemental analysis, we obtained an Ag:O ratio of ~2:1 (Figure 4), which indicates that the nanoparticles we obtained mainly consist of silver (I) Ag 2 O oxide. The modulation interference microscopy method, in contrast to atomic force microscopy (Figure 5), allows one to assess the uneven distribution of nano-and micro-sized particles [70]. Using modulation interference microscopy, we found that NPs were unevenly distributed over the polymer volume (Figure 6).…”

Section: Discussionmentioning

confidence: 96%

A Novel Biodegradable Composite Polymer Material Based on PLGA and Silver Oxide Nanoparticles with Unique Physicochemical Properties and Biocompatibility with Mammalian Cells

et al. 2021

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A method for obtaining a stable colloidal solution of silver oxide nanoparticles has been developed using laser ablation. The method allows one to obtain nanoparticles with a monomodal size distribution and a concentration of more than 108 nanoparticles per mL. On the basis of the obtained nanoparticles and the PLGA polymer, a nanocomposite material was manufactured. The manufacturing technology allows one to obtain a nanocomposite material without significant defects. Nanoparticles are not evenly distributed in the material and form domains in the composite. Reactive oxygen species (hydrogen peroxide and hydroxyl radical) are intensively generated on the surfaces of the nanocomposite. Additionally, on the surface of the composite material, an intensive formation of protein long-lived active forms is observed. The ELISA method was used to demonstrate the generation of 8-oxoguanine in DNA on the developed nanocomposite material. It was found that the multiplication of microorganisms on the developed nanocomposite material is significantly decreased. At the same time, the nanocomposite does not inhibit proliferation of mammalian cells. The developed nanocomposite material can be used as an affordable and non-toxic nanomaterial to create bacteriostatic coatings that are safe for humans.

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Section: Discussionmentioning

confidence: 96%

A Novel Biodegradable Composite Polymer Material Based on PLGA and Silver Oxide Nanoparticles with Unique Physicochemical Properties and Biocompatibility with Mammalian Cells

et al. 2021

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“…One of the most modern, reliable and super-resolving methods that is used for applied and fundamental problems of biomedical research, which makes it possible to quickly assess the morphofunctionality of biopolymer coatings, is interference microscopy [99,100]. At the heart of the modern optical method of modulation laser interference microscopy, the local phases of the modulated object of the light wave are measured and taken into account with full control of the polarization of the region, and the obtained interferograms provided on the photodetector after mathematical processing restore the phase portrait of the portrait field of view ( Figure 5) [101,102]. There are many strategies for effectively solving problems associated with the most accurate and reliable measurement of the thickness of a thin polymer coating [103]-a theoretical calculation or an empirical characteristic of the system on an opaque artifact on the surface or in the thickness of the film, providing a signal model representing the response of the instrument in the absence of a film.…”

Section: Investigation Of the Surface Of Biopolymer Materialsmentioning

confidence: 99%

Biopolymer Matrices Based on Chitosan and Fibroin: A Review Focused on Methods for Studying Surface Properties

Захарова¹,

Кильдеева²

2021

Polysaccharides

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For the creation of tissue-engineered structures based on natural biopolymers with the necessary chemical, physical, adhesive, morphological, and regenerative properties, biocompatible materials based on polysaccharides and proteins are used. This work is devoted to a problem of the technology of polymeric materials for biomedical purposes: the creation of biopolymer tissue engineering matrix and the development of a methodology for studying morphology and functional properties of their surface to establish the prospects for using the material for contact with living objects. The conditions for the formation of scaffolds based on composite materials of chitosan and fibroin determine the structure of the material, the thickness and orientation of molecular layers, the surface morphology, and other parameters that affect cell adhesion and growth. The analysis of studies of the morphology and properties of the surface of biopolymer matrices obtained using different methods of molding from solutions of chitosan and fibroin is carried out.

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“…Study of the nonlinear CSK dynamics using the data of the Laser Interference Microscopy (LIM) is the impact opportunity for the objectification of the cell states and cytological cancer diagnosis analyzing the time series of phase thickness fluctuations (in the "cross-sections" of the nucleus, the nucleolus, cytoplasm). The main advantages of the MIM-340 laser microscope are high resolution in the lateral plane (10-100 nm) and vertical (0.3 nm), the frequency recording of phase images (33 Hz) and the presence of an object table that allows the positioning the object (positioning accuracy is 150 nm) [61]. The results of typical measurements are presented in Fig.…”

Section: Laser Interference Microscopy Of Cell Dynamics Ductile-britmentioning

confidence: 99%

DNA Transformation, Cell Epigenetic Landscape and Open Complex Dynamics in Cancer Development

Naimark¹,

Bayandin²,

Beloglazova³

et al. 2020

Math.Biol.Bioinf.

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Statistical thermodynamics allowed the formulation of mesoscopic approach of DNA transformation in course of the excitation of collective distortion modes (denaturation bubbles) associated with hydrogen bond breaking between the base pairs. Intermediate (non-continual limit) of DNA modeling (the Peyrard-Bishop model) is combined with the field description (generalized Ginzburg-Landau approach) to analyze the dynamics of collective open complex modes associated with mesodefects in the DNA ensemble. Collective modes dynamics describes different scenario of gene expression according to statistically predicted form of out-of-equilibrium potential (epigenetic landscape) reflecting specific type criticality of “soft matter” with mesodefects (open complexes) – the structural-scaling transition. Principal difference of thermodynamics of non-continual and continual models is thermalization conditions related to thermal fluctuations responsible for the DNA breathing (localized excitation with breather dynamics) and structural-scaling parameter responsible for spinodal decomposition of out-of-equilibrium potential metastability due to generation of open complex collective modes. Open complex collective modes have the nature of self-similar solutions (breathers, auto-solitary and blow-up modes) of open complex evolution equation accounting qualitative different types of potential metastabilities. Sub-sets of collective modes represent the phase variables of attractors associated with different scenario of expression dynamics, which allows the interpretation of multistability of the epigenetic landscape and the Huang diagram of gene expression. It was shown different epigenetic pathway in attractors phase space corresponding to normal and cancer expression scenario. These scenarios were supported by laser interference microscopy of living normal and cancer cells illustrating multi- and monofractal dynamics.

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Laser Interference Microscopy for Nanobiotechnologies

Cited by 9 publications

References 10 publications

A Novel Biodegradable Composite Polymer Material Based on PLGA and Silver Oxide Nanoparticles with Unique Physicochemical Properties and Biocompatibility with Mammalian Cells

A Novel Biodegradable Composite Polymer Material Based on PLGA and Silver Oxide Nanoparticles with Unique Physicochemical Properties and Biocompatibility with Mammalian Cells

Biopolymer Matrices Based on Chitosan and Fibroin: A Review Focused on Methods for Studying Surface Properties

DNA Transformation, Cell Epigenetic Landscape and Open Complex Dynamics in Cancer Development

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