Laser speckle contrast imaging and laser Doppler flowmetry reproducibly assess reflex cutaneous vasoconstriction

Schwartz, Kelsey S.; Theis, Emma; Bunting, Kearsten; McCaughey, Rebecca A.; Lang, J.

doi:10.1016/j.mvr.2022.104363

Cited by 8 publications

(9 citation statements)

References 44 publications

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“…One reason for the difference is that an additional operation was implemented to eliminate the possible discontinuity of phase values by jumping from π to

- \pi

or vice versa in this study. Another reason may be related to the better reproducibility and reliability of the LSCI technique compared with LDF, 8 which makes the phase relation of IRT and LSCI spectra statistically clearer.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have shown that LSCI can provide an index of skin blood flow proportional to the concentration and mean velocity of red blood cells, 5 and allow measurement of skin blood flow over a wide area with a high frame rate and good reproducibility. 6,7 In a recent experimental study of reflex cutaneous vasoconstriction, 8 changes in skin blood flow were measured as LSCI and LDF flux. The test-retest results showed that LSCI displayed better reproducibility and reliability compared with LDF throughout the cooling bout and at the plateau, and thus may be more suitable for longitudinal assessments.…”

Section: Introductionmentioning

confidence: 99%

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Spectral analysis of laser speckle contrast imaging and infrared thermography to assess skin microvascular reactive hyperemia

Querleux

Lei

et al. 2023

Skin Research and Technology

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Background Post‐occlusive reactive hyperemia (PORH) test with signal spectral analysis coupled provides potential indicators for the assessment of microvascular functions. Objective The objective of this study is to investigate the variations of skin blood flow and temperature spectra in the PORH test. Furthermore, to quantify the oscillation amplitude response to occlusion within different frequency ranges. Materials and methods Ten healthy volunteers participated in the PORH test and their hand skin temperature and blood flow images were captured by infrared thermography (IRT) and laser speckle contrast imaging (LSCI) system, respectively. Extracted signals from selected areas were then transformed into the time‐frequency space by continuous wavelet transform for cross‐correlation analysis and oscillation amplitude response comparisons. Results The LSCI and IRT signals extracted from fingertips showed stronger hyperemia response and larger oscillation amplitude compared with other areas, and their spectral cross‐correlations decreased with frequency. According to statistical analysis, their oscillation amplitudes in the PORH stage were obviously larger than the baseline stage within endothelial, neurogenic, and myogenic frequency ranges (p < 0.05), and their quantitative indicators of oscillation amplitude response had high linear correlations within endothelial and neurogenic frequency ranges. Conclusion Comparisons of IRT and LSCI techniques in recording the reaction to the PORH test were made in both temporal and spectral domains. The larger oscillation amplitudes suggested enhanced endothelial, neurogenic, and myogenic activities in the PORH test. We hope this study is also significant for investigations of response to the PORH test by other non‐invasive techniques.

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“…One reason for the difference is that an additional operation was implemented to eliminate the possible discontinuity of phase values by jumping from π to

- \pi

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Spectral analysis of laser speckle contrast imaging and infrared thermography to assess skin microvascular reactive hyperemia

Querleux

Lei

et al. 2023

Skin Research and Technology

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“…Laser speckle contrast imaging (LSCI), as a real-time, noncontact, full-field imaging technique with a large field of view, 6 has gained interest as an imaging technique for evaluating microvascular circulation of various tissues, such as retina, cerebrum, skin and liver. [7][8][9][10][11] Despite extensive research on LSCI, few studies have explored its potential as kidney quality assessment tool. The only study that has used LSCI to measure the kidney during NMP, conducted by Heeman et al 12 showed that onesided measurement can assess cortical microcirculation and detect perfusion deficits.…”

Section: Introductionmentioning

confidence: 99%

Real‐time laser speckle contrast imaging measurement during normothermic machine perfusion in pretransplant kidney assessment

Fang,

van Ooijen,

Ambagtsheer

et al. 2023

Lasers Surg Med

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ObjectivesNormothermic machine perfusion (NMP) provides a platform for pre‐transplant kidney quality assessment that is essential for the use of marginal donor kidneys. Laser speckle contrast imaging (LSCI) presents distinct advantages as a real‐time and noncontact imaging technique for measuring microcirculation. In this study, we aimed to assess the value of LSCI in visualizing renal cortical perfusion and investigate the additional value of dual‐side LSCI measurements compared to single aspect measurement during NMP.MethodsPorcine kidneys were obtained from a slaughterhouse and then underwent NMP. LSCI was used to measure one‐sided cortical perfusion in the first 100 min of NMP. Thereafter, the inferior renal artery branch was occluded to induce partial ischemia and LSCI measurements on both ventral and dorsal sides were performed.ResultsLSCI fluxes correlated linearly with the renal blood flow (R2 = 0.90, p < 0.001). After renal artery branch occlusion, absence of renal cortical perfusion could be visualized and semiquantified by LSCI. The overall ischemic area percentage of the ventral and dorsal sides was comparable (median interquartile range [IQR], 38 [24−43]% vs. 29 [17−46]%, p = 0.43), but heterogenous patterns between the two aspects were observed. There was a significant difference in oxygen consumption (mean ± standard deviation [SD], 2.57 ± 0.63 vs. 1.83 ± 0.49 mLO2/min/100 g, p < 0.001), urine output (median [IQR], 1.3 [1.1−1.7] vs. 0.8 [0.6−1.3] mL/min, p < 0.05), lactate dehydrogenase (mean ± SD, 768 ± 370 vs. 905 ± 401 U/L, p < 0.05) and AST (mean ± SD, 352 ± 285 vs. 462 ± 383 U/L, p < 0.01) before and after renal artery occlusion, while no significant difference was found in creatinine clearance, fractional excretion of sodium, total sodium reabsorption and histological damage.ConclusionsLSCI fluxes correlated linearly with renal blood flow during NMP. Renal cortical microcirculation and absent perfusion can be visualized and semiquantified by LSCI. It provides a relative understanding of perfusion levels, allowing for a qualitative comparison between regions in the kidney. Dual‐side LSCI measurements are of added value compared to single aspect measurement and renal function markers.

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“… 11 Instead, vasoconstriction is primarily measured with obtrusive systems, like laser Doppler flowmetry or high-pressure cuffs that eliminate venous pooling. 12 , 13 Thus, a tremendous opportunity to characterize patient health and detect disease is foregone. If one could measure vasoconstriction in real time, it would be possible to assess the effectiveness and adjust the dosage of blood pressure medication, detect serious health conditions, like a sickle cell attack, before it occurs, identify long-term trends in sympathetic neural activity and patient stress, and better understand an individual’s unique response to hormonal medication.…”

Section: Introductionmentioning

confidence: 99%

“… 20 There are a number of rigid and bulky devices that monitor vasoconstriction. 12 , 13 , 21 , 22 , 23 , 24 , 25 , 26 Overall, new studies in soft material interfaces are necessary to unlock chest-based PPG’s medical potential for vasoconstriction detection.…”

Section: Introductionmentioning

confidence: 99%

Soft wireless sternal patch to detect systemic vasoconstriction using photoplethysmography

Zavanelli¹,

Lee²,

Guess³

et al. 2023

iScience

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Laser speckle contrast imaging and laser Doppler flowmetry reproducibly assess reflex cutaneous vasoconstriction

Cited by 8 publications

References 44 publications

Spectral analysis of laser speckle contrast imaging and infrared thermography to assess skin microvascular reactive hyperemia

Spectral analysis of laser speckle contrast imaging and infrared thermography to assess skin microvascular reactive hyperemia

Real‐time laser speckle contrast imaging measurement during normothermic machine perfusion in pretransplant kidney assessment

Soft wireless sternal patch to detect systemic vasoconstriction using photoplethysmography

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