Laser speckle contrast imaging system using nanosecond pulse laser source

Zhao, Yuemei; Wang, Kang; Li, Weitao; Zhang, Huan; Qian, Zhiyu; Liu, Yangyang

doi:10.1117/1.jbo.25.5.056005

Cited by 6 publications

(4 citation statements)

References 23 publications

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“…Then, a stack of 30 raw images was stored and processed. Equation ( 2) was used to calculate speckle contrast (K) [26].…”

Section: The Rcbf Images Obtained By Lscimentioning

confidence: 99%

Research on the classification of early‐stage brain edema by combining intrinsic optical signal imaging and laser speckle contrast imaging

Zhang,

Qi,

et al. 2024

Journal of Biophotonics

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The early detection and pathological classification of brain edema are very important for symptomatic treatment. The dual‐optical imaging system (DOIS) consists of intrinsic optical signal imaging (IOSI) and laser speckle contrast imaging (LSCI), which can acquire cerebral hemodynamic parameters of mice in real‐time, including changes of oxygenated hemoglobin concentration (), deoxyhemoglobin concentration (ΔCHbR) and relative cerebral blood flow (rCBF) within the field of view. The slope sum of , ΔCHbR and rCBF was proposed to classify vasogenic edema (VE) and cytotoxic edema (CE). The slope sum values in the VE and CE group remain statistically different and the classification results provide higher accuracy of more than 93% for early brain edema detection. In conclusion, the differences of hemodynamic parameters between VE and CE in the early stage were revealed and the method helps in the classification of early brain edema.

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“…Then, a stack of 30 raw images was stored and processed. Equation ( 2) was used to calculate speckle contrast (K) [26].…”

Section: The Rcbf Images Obtained By Lscimentioning

confidence: 99%

Research on the classification of early‐stage brain edema by combining intrinsic optical signal imaging and laser speckle contrast imaging

Zhang,

Qi,

et al. 2024

Journal of Biophotonics

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“…A series of works [180][181][182] presents studies of CBF and brain electrophysiology monitoring before, during, and after asphyxial cardiac arrest and further resuscitation using LSCI and LSCI combined with electroencephalography. Zhao et al [183] showed the possibility to use nanosecond pulse laser sources in LSCI setups for CBF measurements. The use of pulsed light sources will possibly allow for combined LSCIphotoacoustic imaging.…”

Section: Brain Vasculature Assessmentmentioning

confidence: 99%

Advances in Dynamic Light Scattering Imaging of Blood Flow

Sdobnov,

Piavchenko,

Bykov

et al. 2023

Laser & Photonics Reviews

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Dynamic light scattering (DLS) is a well known experimental approach uniquely suited for the characterization of small particles undergoing Brownian motion in randomly inhomogeneous turbid scattering medium, including water suspension, polymers in solutions, cells cultures, and so on. DLS is based on the illuminating of turbid medium with a coherent laser light and further analyzes the intensity fluctuations caused by the motion of the scattering particles. The DLS‐based spin‐off derivative techniques, such laser Doppler flowmetry (LDF), diffusing wave spectroscopy (DWS), laser speckle contrast imaging (LSCI), and Doppler optical coherence tomography (DOCT), are exploited widely for non‐invasive imaging of blood flow in brain, skin, muscles, and other biological tissues. The recent advancements in the DLS‐based imaging technologies in frame of their application for brain blood flow monitoring, skin perfusion measurements, and non‐invasive blood micro‐circulation characterization are overviewed. The fundamentals, breakthrough potential, and practical findings revealed by DLS‐based blood flow imaging studies, including the limitations and challenges of the approach such as movement artifacts, non‐ergodicity, and overcoming high scattering properties of studied medium, are also discussed. It is concluded that continued research and further technological advancements in DLS‐based imaging will pave the way for new exciting developments and insights into blood flow diagnostic imaging.

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“…The exposure time of the CCD was set as 20 ms, and the images were acquired through the programming software at 20 Hz. The following equation ( 2) was used to calculate speckle contrast ( K ) [5] .…”

Section: Brain Blood Flow Observation Using Laser Speckle Contrast Im...mentioning

confidence: 99%

A study on the identification of types of cerebral edema by using multi-parametric imaging system

Zhang¹,

Li²,

Qi³

et al. 2022

Optics in Health Care and Biomedical Optics XII

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Cerebral edema is a potentially fatal disorder and divided into several types. The accurately identifying the type of cerebral edema is vital for the precise treatment. Previous studies have been devoted the multi-model parameters to access the feature cerebral edema. However, few research focused on the models to distinguish the types of cerebral edema. Here, a multi-parameter joint imaging system was developed, which combined laser speckle contrast imaging system (LSCI) and intrinsic optical signals imaging system (IOS). Several parameters such as relative cerebral blood flow (rCBF), concentration changes of oxy-hemoglobin (HbO), deoxy-hemoglobin (HbR), and blood oxygen saturation (sO2) were recorded at the same region of interest (ROI). Vasogenic and cytotoxic cerebral edema models were induced by phenylephrine hydrochloride and vasopressin, respectively. The mice were randomly assigned to 2 groups and 10 individuals in each group. After drug injection, the parameters were collected every 20 minutes within 2 h in vivo. The rCBF in both groups increased gradually within 2 hours throughout the whole process, but the rCBF in the vasogenic group grew substantially faster than it in the cytotoxic group within the first 80 minutes. In the vasogenic group, the concentration changes of HbO and HbR were more pronounced. The data was analyzed by the t-test of an independent sample, and there were significant differences between the parameters of the two types of cerebral edema. It has potential for achieving a classification of cerebral edema.

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Laser speckle contrast imaging system using nanosecond pulse laser source

Cited by 6 publications

References 23 publications

Research on the classification of early‐stage brain edema by combining intrinsic optical signal imaging and laser speckle contrast imaging

Research on the classification of early‐stage brain edema by combining intrinsic optical signal imaging and laser speckle contrast imaging

Advances in Dynamic Light Scattering Imaging of Blood Flow

A study on the identification of types of cerebral edema by using multi-parametric imaging system

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