lasR/rhlR Expression Linked to Quorum Sensing-Mediated Biofilm Formation in Pseudomonas aeruginosa Using Gold Nanoparticles Synthesized with Ethnobotanical Extracts

Velasco, Alexander T.; Fernando, Somar Israel D.; Cruz, Khristina G. Judan

doi:10.1007/s12668-020-00757-7

Cited by 4 publications

(10 citation statements)

References 37 publications

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“…Kruskal-Wallis test (non-parametric ANOVA) where means between the control and experimental set-up were compared and the significance determined if the F-values was greater than the F-crit at 0.05 level of significance was used. Statistical analysis of the critical threshold value was done through ANOVA (Velasco et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…The protocols of Tan et al, (2013) and Srisawat (2007) as followed by Velasco et al, (2020) were used for ethanol extraction with modifications. The plant samples were cleaned using distilled water and 70% (v/v) ethanol then air-dried.…”

Section: Article Infomentioning

confidence: 99%

“…After 24 to 48 hrs period of incubation, the antibacterial activity of the plant extracts was noted for the appearance of zone of inhibition. Plant extracts should not exhibit zone of inhibition which was required for accuracy of the subsequent assay to rule out antibacterial-mediated decrease in virulence factor production (Fernando and Judan Cruz, 2020;Velasco et al, 2020).…”

Section: Antibacterial Assay Of Plant Extracts Against P Aeruginosamentioning

confidence: 99%

“…To remove planktonic cells, the plates were rinsed with sterile distilled water and air dried for 45 minutes. For staining, 150 μl of 1% crystal violet solution in water for 45 minutes was used (Fernando and Judan Cruz, 2020;Velasco et al, 2020). For quantification of biofilm, 200 μl of 95% ethanol was added to destain the wells.…”

Section: Microtitre Plate Biofilm Formation Assaymentioning

confidence: 99%

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PHILIPPINE ETHNOBOTANICALS DOWNREGULATE lasR EXPRESSION LINKED TO QUORUM SENSING-MEDIATED BIOFILM FORMATION IN Pseudomonas aeruginosa

Santos¹,

Jacinto²,

Cruz

2021

J microb biotech food sci

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The prevalence of antibacterial resistance has brought about a growing surge to develop novel approaches to control infectious diseases. Targeting Quorum-sensing (QS) - controlled virulence factors in bacteria has indicated a promising strategy for antipathogenic drugs. Extracts of Philippine Ilongot-Egongot ethnobotanicals Stachytarpeta jamaicensis, Adenanthera intermedia, Mikania micrantha, Hyptis suaveolens, Premna odorata, Cymbopogan winterianus, Phyllanthus urinaria, Dillenia philippinensis, Hydrocotyle vulgaris, Senna alata, Urena lobata, Ceiba pentandra, Ficus sp., Eleusine indica, Diplazium esculentum and Talahib (no known scientific name) were screened using microtiter plate biofilm formation assay for their QS inhibition activity against biofilm formation in Pseudomonas aeruginosa clinical isolate and Pseudomonas aeruginosa PNCM 1335. Extracts of M. micrantha, H. suaveolens flowers, H. vulgaris, A. intermedia, E. indica leaves, D. esculentum and Talahib have inhibitory effect on P. aeruginosa clinical isolate biofilm formation. Decrease in biofilm formation was shown in extracts of S. jamaicensis., M. micrantha, H. suaveolens, H. vulgaris, U. lobata, C. pentandra, A. intermedia, E. indica, D. esculentum and Talahib. against P. aeruginosa PNCM 1335. RNA extracts of M. micrantha, Talahib and A. intermedia exhibited downregulation of lasR in both test bacteria using qRT-PCR analysis through absolute quantification. The prospects of these ethnobotanicals to inhibit bacterial virulence avoiding antibiotic resistance is presented in this paper.

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Section: Discussionmentioning

confidence: 99%

Section: Article Infomentioning

confidence: 99%

Section: Antibacterial Assay Of Plant Extracts Against P Aeruginosamentioning

confidence: 99%

Section: Microtitre Plate Biofilm Formation Assaymentioning

confidence: 99%

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PHILIPPINE ETHNOBOTANICALS DOWNREGULATE lasR EXPRESSION LINKED TO QUORUM SENSING-MEDIATED BIOFILM FORMATION IN Pseudomonas aeruginosa

Santos¹,

Jacinto²,

Cruz

2021

J microb biotech food sci

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“…These plants are powerful sources of natural QS inhibitors essential for the development of safe, novel therapeutic and antipathogenic agents ( Fernando and Judan Cruz, 2020 ). Recently, the Ilongot-Eǵongot ethnobotanicals evaluated in this study have been demonstrated to possess QS inhibition properties against pathogenic bacteria such as Pseudomonas aeruginosa ( Velasco et al, 2020 ; Santos et al, 2021 ), Staphylococcus aureus ( Salamanca et al, 2019 ), Aeromonas hydrophila ( Fernando and Judan Cruz, 2020 ), and Streptococcus agalactiae ( Fernando et al, 2020 ). QSI actions of these plants against pathogenic fungi such as Candida have not yet been explored.…”

Section: Introductionmentioning

confidence: 99%

Candida albicans Biofilm Inhibition by Ethnobotanicals and Ethnobotanically-Synthesized Gold Nanoparticles

et al. 2021

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The virulence and drug resistance of globally prevalent Candida albicans has presented complications toward its control while advances in effective antivirulence drugs remain critical. Emerging methods are now being evaluated to facilitate development of novel therapeutic approaches against this pathogen. This study focuses on the biofilm formation inhibition of ethnobotanical crude extracts and the use of nanotechnology through the ethnobotanically-synthesized gold nanoparticles to control C. albicans. Control on biofilm formation was compared using crude extracts (CEs) and biologically synthesized gold nanoparticles (CEs + AuNPs). Significantly lower biofilm formation was exhibited in thirteen (13) CEs and fourteen (14) CEs + AuNPs. Biofilm-linked genes Bcr1 and HSP90 expression were consequently downregulated. Higher biofilm inhibition activity was noted in some CEs + AuNPs compared to its counterpart CEs. This study emphasizes the biofilm inhibition activity of ethnobotanicals and the use of nanoparticles to enhance delivery of compounds, and points to its prospects for developing anti-pathogenic drugs without evolving resistance.

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Modulation of quorum sensing activity by copper sulfate, potassium dichromate, and cadmium chloride in biosensor strains

Torres

Palacios

Lacosegliaz

et al. 2022

Letters in Applied Microbiology

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Beyond their biological roles, metals have a strong impact on the environment. It has been reported that metals are also inhibitory of Quorum Sensing (QS) mechanisms, ones of the best characterized signaling systems in bacteria and fungi. We analyzed the effect of CuSO4, CdCl2, and K2Cr2O7, on QS systems sharing or differing in the bacterial host or the QS signal. The results in this study show that CuSO4 can not only be inhibitory, but also stimulatory of QS activity: at 0.2 mM increased six fold the activity in Chromobacterium subtsugae CV026. This behavior is related to the concentration of the metal and the particular QS system: E. coli MT102 (pJBA132) was no affected, but CuSO4 decreased the QS activity of Pseudomonas putida F117 (pKR-C12) to half its control values. K2Cr2O7 increased four and three folds the QS activities of E. coli MT102 (pJBA132) and P. putida F117 (pAS-C8), respectively, but without effect when combined with CuSO4 or CdCl2. CdCl2 only showed a positive effect in CV026 when combined with CuSO4. Results suggest that factors related with the culture conditions impact on the influence of the metals, and reinforce the importance of the environment in the modulation of QS activity.

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lasR/rhlR Expression Linked to Quorum Sensing-Mediated Biofilm Formation in Pseudomonas aeruginosa Using Gold Nanoparticles Synthesized with Ethnobotanical Extracts

Cited by 4 publications

References 37 publications

PHILIPPINE ETHNOBOTANICALS DOWNREGULATE lasR EXPRESSION LINKED TO QUORUM SENSING-MEDIATED BIOFILM FORMATION IN Pseudomonas aeruginosa

PHILIPPINE ETHNOBOTANICALS DOWNREGULATE lasR EXPRESSION LINKED TO QUORUM SENSING-MEDIATED BIOFILM FORMATION IN Pseudomonas aeruginosa

Candida albicans Biofilm Inhibition by Ethnobotanicals and Ethnobotanically-Synthesized Gold Nanoparticles

Modulation of quorum sensing activity by copper sulfate, potassium dichromate, and cadmium chloride in biosensor strains

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