In vivo characterization of soft tissue is a key step towards accurate biomechanical simulation enabling planning and intra-operative assisted surgery. This chapter presents the new version of LASTIC, a device measuring soft tissue deformations using a negative pressure. Its capabilities are compared with standard tensile tests on five samples with different elastic properties, i.e. Young modulus, from 10 kPa to 1 MPa, in order to estimate its accuracy and define the functional measurement range. Results show that LASTIC overestimates Young modulus by an average of 24% compared to the tensile devices. This error, although rather large,