Abstract:Selective
discrimination and lasting tracking of live bacteria
are primary steps for microbiology research and treatment of bacterial
infection. However, conventional detection methods, such as the gold
standard of Gram staining, are being challenged under actual test
conditions. Herein, we provided a novel method, namely, three excitation
peaks and single-color emission carbon quantum dots (T-SCQDs) for
the rapid (5 min) peptidoglycan-targeting discrimination of Gram-positive
bacteria and lasting tracking (24… Show more
“…S6 ) . In the curve of gCDs (red line) and gCDs-E (purple line), absorption bands at 1642 cm −1 , 1108 cm −1 are corresponding to the stretching vibration of C O and C–O [ 47 , 61 ], respectively. Therefore, hydroxyl and carboxyl are the main functional groups on the surface of gCDs and gCDs-E. Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Considering the hampering effect of the BBB, designed nanocarriers with the ability to anchor EGCG and overcome the BBB are required. Fortunately, carbon dots (CDs) have greatly attracted our attention due to their ability of crossing the BBB, unique optical property and low toxicity [ [43] , [44] , [45] , [46] , [47] ]. For example, Xiao and Wang et al.…”
Amyloid aggregation and fungal infection, especially amyloid beta (A
β
) peptide and
Candida albicans
are considered as two of the crucial pathogenic agents in Alzheimer's disease (AD). In this work, we propose an innovative treatment strategy for AD, targeting at not only A
β
aggregation but also
Candida albicans
infection. Here, a high-performance nanomaterial, namely gCDs-E, have been prepared by self-assembled of glycosylated carbon dots (gCDs) and epigallocatechin-3-gallate (EGCG). Surprisingly, gCDs-E can not only suppress the fibrillation of A
β
and disaggregate A
β
fibrils, but also effectively inhibit the activity of
Candida albicans
. More importantly, the prepared gCDs-E can effectively cut down the cytotoxicity of amyloid aggregations, and the cell viability reached to 99.2%. In addition, the capability of the gCDs-E for blood brain barrier (BBB) penetration was also observed using a normal mice model. Above all, the gCDs-E greatly cleaned A
β
deposition and improved memory impairment in APP/PS1 transgenic AD model mice, confirming its potential as therapeutic agent for AD treatment.
“…S6 ) . In the curve of gCDs (red line) and gCDs-E (purple line), absorption bands at 1642 cm −1 , 1108 cm −1 are corresponding to the stretching vibration of C O and C–O [ 47 , 61 ], respectively. Therefore, hydroxyl and carboxyl are the main functional groups on the surface of gCDs and gCDs-E. Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Considering the hampering effect of the BBB, designed nanocarriers with the ability to anchor EGCG and overcome the BBB are required. Fortunately, carbon dots (CDs) have greatly attracted our attention due to their ability of crossing the BBB, unique optical property and low toxicity [ [43] , [44] , [45] , [46] , [47] ]. For example, Xiao and Wang et al.…”
Amyloid aggregation and fungal infection, especially amyloid beta (A
β
) peptide and
Candida albicans
are considered as two of the crucial pathogenic agents in Alzheimer's disease (AD). In this work, we propose an innovative treatment strategy for AD, targeting at not only A
β
aggregation but also
Candida albicans
infection. Here, a high-performance nanomaterial, namely gCDs-E, have been prepared by self-assembled of glycosylated carbon dots (gCDs) and epigallocatechin-3-gallate (EGCG). Surprisingly, gCDs-E can not only suppress the fibrillation of A
β
and disaggregate A
β
fibrils, but also effectively inhibit the activity of
Candida albicans
. More importantly, the prepared gCDs-E can effectively cut down the cytotoxicity of amyloid aggregations, and the cell viability reached to 99.2%. In addition, the capability of the gCDs-E for blood brain barrier (BBB) penetration was also observed using a normal mice model. Above all, the gCDs-E greatly cleaned A
β
deposition and improved memory impairment in APP/PS1 transgenic AD model mice, confirming its potential as therapeutic agent for AD treatment.
“…Besides hemolysis rate, the major organs including the hearts, livers, spleens, lungs, and kidneys were collected and stained with H&E for further evaluation of biocompatibility as previously reported [17].…”
Photodynamic antimicrobial chemotherapy (PACT) is a promising therapy against biofilm infection. However, due to the saliva clearance and obstacle of biofilm, the photosensitizer is difficult to concentrate in the infection site; then, the PACT is less effective on oral biofilm infection. In this article, we report a special nano-antibacterial agent (SiO2-PCe6-IL) to solve the bottleneck problem of PACT in treatment of oral biofilm infections. The SiO2-PCe6-IL was composed of SiO2 and poly ionic liquid photosensitizer (PCe6-IL) and had tri-fold features of eliminate biofilm infection: high binding ability, breaking biofilm barriers, and enrichment photosensitizer in infection site. In oral biofilm, the SiO2-PCe6-IL changed to SiO2-PIL+ like claws of octopus that could hold tightly with biofilm. Then, the poly-dodecyl on the SiO2-PIL+ broke down the barrier of biofilm. The results of HR-MS and zeta potential indicated that SiO2-PCe6-IL could change to positive (SiO2-PIL+) in acidic environment. The interaction forces and morphology results proved that the SiO2-PIL+ had a higher affinity to biofilm and could destroy the biofilm structure. Then, the photosensitizer was enriched in biofilm at sites of infection. The in vitro and in vivo experiments showed that SiO2-PCe6-IL could effectively eradicate oral biofilm infections and control of dental caries.
“…Due to the influence of precursors, the surface of CQDs is covered with hydrophilic functional groups such as -COOH and -OH, and the particle sizes are small, which endows them with the potential for good biocompatibility. [33][34][35] Besides, the hydrophilic groups on the surface of CQDs promote the interaction with proteins in vivo, resulting in the formation of protein corona, which changes the original properties of CQDs. 36,37 Therefore, how to change the surface properties of CQDs to realize the controllable load of proteins and how to regulate the interaction between the two has become one of the hot topics that many scientists compete to study.…”
Carbon quantum dots (CQDs) are an emerging class of quasi-zero-dimensional photoluminescent nanomaterials with particle sizes less than 10 nm. Owing to their favourable water dispersion, strong chemical inertia, stable optical performance, and good biocompatibility, CQDs have become prominent in biomedical fields. CQDs can be fabricated by "top-down" and "bottom-up" methods, both of which involve oxidation, carbonization, pyrolysis and polymerization. The functions of CQDs include biological imaging, biosensing, drug delivery, gene carrying, antimicrobial performance, photothermal ablation and so on, which enable them to be utilized in antitumour applications. The purpose of this review is to summarize the research progress of CQDs in antitumour applications from preparation and characterization to application prospects. Furthermore, the challenges and opportunities of CQDs are discussed along with future perspectives for precise individual therapy of tumours.
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