Late blight on potato is suppressed by the biosurfactant-producing strain Pseudomonas koreensis 2.74 and its biosurfactant

Hultberg, Malin; Bengtsson, Tommy; Liljeroth, Erland

doi:10.1007/s10526-010-9289-7

Cited by 58 publications

(38 citation statements)

References 29 publications

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“…The presence of similar constituent analogues in both the rhamnolipids highlights their key role in the results obtained in the microtitre assay against C. falcatum spore germination. Hultberg et al reported that no reduction in mycelial growth rate of Phytophthora infestans was observed for the biosurfactant produced by Pseudomonas koreensis 2.74 at concentrations of 50, 100, and 200 μg ml −1 . Only at the highest concentration (1000 μg ml −1 ), significant reduction in growth rate was observed.…”

Section: Discussionmentioning

confidence: 99%

Antifungal properties of rhamnolipid produced by Pseudomonas aeruginosa DS9 against Colletotrichum falcatum

Goswami

Borah

Lahkar

et al. 2015

J. Basic Microbiol.

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The rhamnolipid biosurfactant (RL-DS9) extracted from the bacterial strain Pseudomonas aeruginosa DS9 was evaluated for its antifungal activity against Colletotrichum falcatum that causes red rot in sugarcane. The surface tension (ST) reduction, biosurfactant production, and antifungal activity of biosurfactant against C. falcatum were investigated by using the medium with different carbon sources and it was found to be maximum in glucose. Moreover, highest reduction of ST and production of biosurfactant was achieved at 4.5% (w/v) concentration of glucose. The efficacy of RL-DS9 was compared with a commercially available rhamnolipid (RL-R95) using microtitre plate assay. Results showed that at 100 μg ml(-1) concentration RL-DS9 exhibited 86.6% inhibition against C. falcatum spore germination, and in the same concentration RL-R95 showed 83.3% inhibition. From liquid chromatography-mass spectrometry (LC-MS) analysis, it was revealed that only two similar congeners Rha-(C10 ) and Rha-Rha-(C10:1 ) were found to be in common among both the rhamnolipids. In the plant bioassay test, it was noted that red rot incidence was reduced on the sugarcane plants treated with RL-DS9. This is the first report that rhamnolipid biosurfactant produced by Pseudomonas aeruginosa DS9 could be able to control red rot disease of sugarcane caused due to the infection with the fungus Colletotrichum falcatum.

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Section: Discussionmentioning

confidence: 99%

Antifungal properties of rhamnolipid produced by Pseudomonas aeruginosa DS9 against Colletotrichum falcatum

Goswami

Borah

Lahkar

et al. 2015

J. Basic Microbiol.

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“…In addition, as pointed out by Hultberg et al (11), the use of nonpathogenic microorganisms is a fundamental and essential issue for biological control. Among Pseudomonas , 23 and 16 species have been shown to exhibit pathogenicity against plants and animals (including humans), respectively (10).…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity and Ecological Evaluation of Fluorescent Pseudomonads Isolated from the Leaves and Roots of Potato Plants

et al. 2012

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A total of 828 isolates of fluorescent pseudomonads (FPs) were obtained from the leaves (305 isolates) and roots (523 isolates) of potato plants grown in different geographical locations in Japan, and 16S rRNA gene sequences of 776 isolates were successfully determined by direct PCR sequencing. Clustering analysis (≥99% identity) identified 13 and 26 operational taxonomic units (OTUs) for leaf- and root-associated FPs, respectively, and 29 OTUs were identified in the phytosphere of potato plants. Among them, 7 and 9 OTUs showed a significantly biased distribution to the leaves and roots, respectively. Phylogenetic analysis revealed that 3 dominant OTUs for leaf-associated FPs were grouped in a cluster of leaf-associated pathogens, such as Pseudomonas cichorii and Pseudomonas viridiflava. In contrast, 4 OTUs were located in a cluster of saprophytic pseudomonads. Among them, 3 OTUs showed high similarity to Pseudomonas koreensis and Pseudomonas vancouverensis, both of which have been reported to be beneficial for biological control or plant growth promotion. These data provide key information for efficient surveying and utilization of beneficial FPs in agricultural practices.

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“…It too exhibited antagonistic activities: a biosurfactant was active both against Phytium ultimum in a tomato model system (21) and against Phytophthora infestans infecting potato plants (22). Another P. koreensis strain, CRS05-R05, was isolated from the rice rhizosphere and showed biocontrol activity against the rice weevil Sitophilus oryzae (23), a pest of stored foods.…”

Section: Introductionmentioning

confidence: 99%

Pushing the limits of de novo genome assembly for complex prokaryotic genomes harboring very long, near identical repeats

Schmid

Frei

Patrignani

et al. 2018

Preprint

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Generating a complete, de novo genome assembly for prokaryotes is often considered a solved problem. However, we here show that Pseudomonas koreensis P19E3 harbors multiple, near identical repeat pairs up to 70 kilobase pairs in length. Beyond long repeats, the P19E3 assembly was further complicated by a shufflon region. Its complex genome could not be de novo assembled with long reads produced by Pacific Biosciences' technology, but required very long reads from the Oxford Nanopore Technology. Another important factor for a full genomic resolution was the choice of assembly algorithm.Importantly, a repeat analysis indicated that very complex bacterial genomes represent a general phenomenon beyond Pseudomonas . Roughly 10% of 9331 complete bacterial and a handful of 293 complete archaeal genomes represented this dark matter for de novo genome assembly of prokaryotes. Several of these dark matter genome assemblies contained repeats far beyond the resolution of the sequencing technology employed and likely contain errors, other genomes were closed employing labor-intense steps like cosmid libraries, primer walking or optical mapping. Using very long sequencing reads in combination with assemblers capable of resolving long, near identical repeats will bring most prokaryotic genomes within reach of fast and complete de novo genome assembly.

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Late blight on potato is suppressed by the biosurfactant-producing strain Pseudomonas koreensis 2.74 and its biosurfactant

Cited by 58 publications

References 29 publications

Antifungal properties of rhamnolipid produced by Pseudomonas aeruginosa DS9 against Colletotrichum falcatum

Antifungal properties of rhamnolipid produced by Pseudomonas aeruginosa DS9 against Colletotrichum falcatum

Genetic Diversity and Ecological Evaluation of Fluorescent Pseudomonads Isolated from the Leaves and Roots of Potato Plants

Pushing the limits of de novo genome assembly for complex prokaryotic genomes harboring very long, near identical repeats

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