Latent class analysis of human herpesvirus 8 assay performance and infection prevalence in sub-Saharan Africa and Malta

Engels, Eric A.; Sinclair, Michael; Biggar, Robert J.; Whitby, Denise; Ebbesen, Peter; Goedert, James J.; Gastwirth, Joseph L.

doi:10.1002/1097-0215(20001215)88:6<1003::aid-ijc26>3.0.co;2-9

Cited by 80 publications

(41 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In east and central Africa HHV-8 prevalence in adults was reported to be 70-90% (Sonja J. Olsen, 1998;Engels EA, 2000). The above findings are comparable with our study where we had 70% for all the subjects and 87% in the HIV infected population and 48.8% in the HIV non-infected population.…”

Section: Discussionsupporting

confidence: 90%

Prevalence of HHV-8 Antibodies among HIV Infected Patients

Ukonu¹,

Eze²

2011

GJHS

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 90%

Prevalence of HHV-8 Antibodies among HIV Infected Patients

Ukonu¹,

Eze²

2011

GJHS

View full text Add to dashboard Cite

“…Of these, three studies identified evidence of KSHV-DNA in peripheral blood cells (Chang et al, 1996;Kasolo et al, 1997;Belec et al, 1998), two used a lytic antibody assay alone (He et al, 1998;Lampinen et al, 2000), five used a latent antibody assay alone Ariyoshi et al, 1998;Bestetti et al, 1998;Bourboulia et al, 1998;Sitas et al, 1999) and 18 used both a lytic and a latent assay (Lennette et al, 1996;Simpson et al, 1996;Mayama et al, 1998;Olsen et al, 1988;Ablashi et al, 1999;Andreoni et al, 1999Andreoni et al, , 2002Enbom et al, 1999;Gessain et al, 1999;Wilkinson et al, 1999;Engels et al, 2000;Rezza et al, 2000;Gaye Serraino-Diallo et al, 2001;Kakoola et al, 2001;Nuvor et al, 2001;Serraino et al, 2001;Wawer et al, 2001). The results are presented in Table 1, together with the estimated cumulative incidence of Kaposi's sarcoma in men aged 0 -64 years in the period before HIV infection (Cook-Mozaffari et al, 1998).…”

Section: Resultsmentioning

confidence: 99%

Review of the distribution of Kaposi's sarcoma-associated herpesvirus (KSHV) in Africa in relation to the incidence of Kaposi's sarcoma

2003

View full text Add to dashboard Cite

In the years before human immunodeficiency virus (HIV) infection, the incidence of Kaposi's sarcoma varied markedly across the African continent, and it was a disease primarily affecting men. In contrast, the evidence reviewed here shows that the causal virusKaposi's sarcoma associated herpesvirus (KSHV) -is prevalent in many African countries, including places where Kaposi's sarcoma was almost unknown before HIV, and that it is as common in women as in men. Therefore, the geographical distribution of Kaposi's sarcoma in Africa before the spread of HIV and its predominance as a disease affecting men are not a simple reflection of the distribution of KSHV. Since the epidemic of HIV in Africa, Kaposi's sarcoma has become relatively more frequent in women, and the incidence has increased in countries where it was previously rare, but where KSHV is prevalent, as well as in countries where it was already common. These changes point to a role for other (as yet unknown) factors in the aetiology of Kaposi's sarcoma that may have the most effect in the absence of concurrent HIV infection.

show abstract

“…LCA has already been used to assess KSHV serological assays in U.S. (27), African, and Mediterranean populations (12). In one study (12), the final LCA model indicated that the ORF K8.1 ELISA had a higher sensitivity and specificity (100% and 98%, respectively) than did ORF 65 ELISA (76% and 57%, respectively) and IFA-LANA (88% and 69%, respectively). However, no clinical or serological reference standards were used in that study to confirm the results of the LCA model.…”

Section: Discussionmentioning

confidence: 99%

Comparative Study of Kaposi's Sarcoma-Associated Herpesvirus Serological Assays Using Clinically and Serologically Defined Reference Standards and Latent Class Analysis

et al. 2007

View full text Add to dashboard Cite

Accurate determination of infection with Kaposi's sarcoma-associated herpesvirus (KSHV) has been hindered by the lack of a "gold standard" for comparison of serological assays used to estimate KSHV prevalence in serosurveys conducted in different settings. We have evaluated the performance of five in-house (developed at University College London [UCL], United Kingdom, and at the virology laboratory of the Instituto de Medicine Tropical [IMT] in Sao Paulo, Brazil) and two commercial (ABI and DIAVIR) serological assays to detect antibodies to latency-associated nuclear antigen (LANA) and to lytic KSHV antigens. We used a variety of serum samples assembled to represent populations likely to be at high, intermediate, and low risk of KSHV infection in Brazil. Composite reference standard panels were prepared based on clinical and serological parameters, against which assay performances were assessed using conventional Bayesian statistics and latent class analysis (LCA). Against the clinical reference standard, in-house immunofluorescence assays to detect anti-LANA antibodies (IFA-LANA) produced at UCL and IMT had similar performances, with sensitivities of 61% (95% confidence interval [CI], 48% to 74%) and 72% (95% CI, 58% to 83%) and specificities of 99% (95% CI, 94% to 100%) and 100% (95% CI, 96% to 100%), respectively, and only the IMT IFA-LANA was included in LCA, together with the IMT IFA-lytic and four enzyme-linked immunosorbent assays (ELISAs). The LCA indicated that the IMT whole-virus ELISA performed best (sensitivity, 87% [95% CI, 81% to 91%]; and specificity, 100% [95% CI, 98% to 100%]), confirming the results obtained with the conventional statistical approach. Commercially available ELISA-based tests yielded the lowest specificities using a spectrum of serum samples. The evaluation of KSHV serological assays is warranted before planning serosurveys in various settings.

show abstract

Latent class analysis of human herpesvirus 8 assay performance and infection prevalence in sub-Saharan Africa and Malta

Cited by 80 publications

References 38 publications

Prevalence of HHV-8 Antibodies among HIV Infected Patients

Prevalence of HHV-8 Antibodies among HIV Infected Patients

Review of the distribution of Kaposi's sarcoma-associated herpesvirus (KSHV) in Africa in relation to the incidence of Kaposi's sarcoma

Comparative Study of Kaposi's Sarcoma-Associated Herpesvirus Serological Assays Using Clinically and Serologically Defined Reference Standards and Latent Class Analysis

Contact Info

Product

Resources

About