Lateral flow assays for detection of disease biomarkers

Gumus, Eda; Bingöl, Haluk; Zor, Erhan

doi:10.1016/j.jpba.2022.115206

Cited by 24 publications

(14 citation statements)

References 192 publications

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“…29,30 AuNPs-LFB strips have been extensively used to diagnose various analytes, such as infectious pathogens, cancer biomarkers, and foodborne microbes. 18 Here, the AuNPs-LFB diagnostic platform comprised…”

Section: Discussionmentioning

confidence: 99%

“…Gold‐nanoparticles‐based lateral flow biosensor (AuNPs‐LFB) is a paper‐based platform that has potential as an advanced POC diagnostic approach owing to its ease of operation, high sensitivity, low cost, and rapid detection 16,17 . This approach has become widespread in biomedical, agricultural, food safety, and environmental fields within a short period 18,19 …”

Section: Introductionmentioning

confidence: 99%

“…16,17 This approach has become widespread in biomedical, agricultural, food safety, and environmental fields within a short period. 18,19 In the current study, the reverse transcription MCDA was integrated with a gold-nanoparticles-based lateral flow biosensor (RT-MCDA-AuNPs-LFB) to develop a novel assay for rapid and visual identification of HCV targeting the gene encoding the 5′untranslated region (5'-UTR), 20 which showed has no homology to other microbes in BLAST searches of the GenBank database. The feasibility of our diagnostic system was verified with clinical samples from patients with suspected HCV infection.…”

Section: Introductionmentioning

confidence: 99%

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Biosensor‐based multiple cross displacement amplification platform for visual and rapid identification of hepatitis C virus

Chen,

Dong,

Shi

et al. 2024

Journal of Medical Virology

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Hepatitis C remains a global health problem, especially in poverty‐stricken areas. A rapid and sensitive point‐of‐care (POC) diagnostic tool is critical for the early detection and timely treatment of hepatitis C virus (HCV) infection. Here, for the first time, we reported a novel molecular diagnostic assay, termed reverse transcription multiple cross displacement amplification integrated with a gold‐nanoparticle‐based lateral flow biosensor (RT‐MCDA‐AuNPs‐LFB), which was developed for rapid, sensitive, specific, and visual identification of HCV. HCV‐RT‐MCDA induced rapid isothermal amplification through a specific primer set targeting the 5′untranslated region gene from the major HCV genotypes 1b, 2a, 3b, 6a, and 3a that are prevalent in China. The optimal reaction temperature and time for RT‐MCDA‐AuNPs‐LFB were 68°C and 25 min, respectively. The limit of detection of the assay was 10 copies per test, and the specificity was 100% for the experimental strains. The whole detection procedure, including crude nucleic acid isolation (~5 min), RT‐MCDA (68°C, 25 min), and visual AuNPs‐LFB result confirmation (less than 2 min), was performed within 35 min. The preliminary results indicated that the HCV‐RT‐MCDA‐AuNPs‐LFB assay could be a valuable tool for sensitive, specific, visual, cost‐saving, and rapid detection of HCV and has potential as a POC diagnostic platform for field screening and early clinical detection of HCV infection.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biosensor‐based multiple cross displacement amplification platform for visual and rapid identification of hepatitis C virus

Chen,

Dong,

Shi

et al. 2024

Journal of Medical Virology

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“…Among the numerous diagnostic techniques available, reverse dot/line blot and lateral flow assays (LFA) have gained significant attention for their simplicity, cost‐effectiveness, and rapidity. The first one has proven valuable in identifying mutations related to hereditary diseases and cancer‐associated genes, 83 while LFAs was mostly used for protein detection, especially antigens 84 . Below, we describe both techniques by providing their principles, examples, advantages as well as drawbacks.…”

Section: Reverse Dot Blots and Lateral Flow Assays—ideal For Low Reso...mentioning

confidence: 99%

“…The first one has proven valuable in identifying mutations related to hereditary diseases and cancer-associated genes, 83 while LFAs was mostly used for protein detection, especially antigens. 84 Below, we describe both techniques by providing their principles, examples, advantages as well as drawbacks.…”

Section: Reverse Dot Blots and Lateral Flow Assays-ideal For Low Reso...mentioning

confidence: 99%

Advanced technologies towards improved HPV diagnostics

Bartosik,

Moranova,

Izadi

et al. 2024

Journal of Medical Virology

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Persistent infection with high‐risk types of human papillomaviruses (HPV) is a major cause of cervical cancer, and an important factor in other malignancies, for example, head and neck cancer. Despite recent progress in screening and vaccination, the incidence and mortality are still relatively high, especially in low‐income countries. The mortality and financial burden associated with the treatment could be decreased if a simple, rapid, and inexpensive technology for HPV testing becomes available, targeting individuals for further monitoring with increased risk of developing cancer. Commercial HPV tests available in the market are often relatively expensive, time‐consuming, and require sophisticated instrumentation, which limits their more widespread utilization. To address these challenges, novel technologies are being implemented also for HPV diagnostics that include for example, isothermal amplification techniques, lateral flow assays, CRISPR‐Cas‐based systems, as well as microfluidics, paperfluidics and lab‐on‐a‐chip devices, ideal for point‐of‐care testing in decentralized settings. In this review, we first evaluate current commercial HPV tests, followed by a description of advanced technologies, explanation of their principles, critical evaluation of their strengths and weaknesses, and suggestions for their possible implementation into medical diagnostics.

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From Pregnancy to Pathogens: Boosting Lateral Flow Assays Sensitivity with a Hydrogel Reaction Trap

Mahardika,

Shin,

Yukird

et al. 2024

Adv Materials Inter

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Lateral Flow Assays (LFAs) are cost‐effective and widely utilized for rapid diagnostics, yet they often suffer from limited sensitivity. This study introduces a straightforward yet highly effective method to enhance LFAs performance by integrating a poly(ethylene glycol) diacrylate (PEGDA) hydrogel to create a “reaction trap.” This hydrogel reaction trap optimizes the flow rate and reaction time at the sensing components, substantially improving assay performance. By applying various hydrogel concentrations (6%, 9%, 12%, 15%, and 18% w/v), significant enhancements across a range of detection systems are achieved. An optimized 18% hydrogel concentration shows a 1.5 times increase in sensitivity in the tested commercial pregnancy test. Additionally, 12% hydrogel concentration is tested in pregnancy, ovulation, and Coronavirus disease 2019 (COVID‐19) commercial kits, and the improvement reached up to a sevenfold increase in signal intensity. The enhancement in detection illustrates the profound impact of this simple modification and shows the importance of hydrogel concentration optimization to maximize detection improvement. These results demonstrate that hydrogel‐coated LFAs offer a scalable and highly effective solution for boosting the reliability and sensitivity of rapid diagnostics across different healthcare settings, with broad potential for global health diagnostics applications.

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Lateral flow assays for detection of disease biomarkers

Cited by 24 publications

References 192 publications

Biosensor‐based multiple cross displacement amplification platform for visual and rapid identification of hepatitis C virus

Biosensor‐based multiple cross displacement amplification platform for visual and rapid identification of hepatitis C virus

Advanced technologies towards improved HPV diagnostics

From Pregnancy to Pathogens: Boosting Lateral Flow Assays Sensitivity with a Hydrogel Reaction Trap

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