Lateral flow assays for food analyses: Food contaminants, allergens, toxins, and beyond

İnce, Bahar; Uludağ, İnci; Demirbakan, Burçak; Özyurt, Canan; Özcan, Burcu; Sezgintürk, Mustafa Kemal

doi:10.1016/j.trac.2023.117418

Cited by 9 publications

(1 citation statement)

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“…), fish, eggs, nuts, and wheat, are responsible for 90% of food allergic reactions. Therefore, the Food and Agriculture Organization of the United Nations (FAO)/World Health Organization (WHO) defined it as the most common food allergens. − Ara h1, the predominant allergenic protein in peanuts, is specifically acknowledged as a major concern owing to its distinctive trimeric structural configuration and widespread distribution characteristics. , The anaphylaxis triggered by Ara h1 is a typical food allergy reaction, which occurs both in children and adults, and even trace amounts of Ara h1 can cause severe allergic symptoms in allergic individuals. − So far, food allergies still cannot be completely cured, making stringent avoidance of allergen-containing foods is the preferred strategy to safeguard allergic individuals from anaphylaxis. , Thus, it is an urgent need to develop an efficient method to trace analysis of Ara h 1 in food for allergen labeling, dietary guidance, and ultimately preventing allergic incidents. − …”

Section: Introductionmentioning

confidence: 99%

Ratiometric Fluorescence Aptasensor of Allergen Protein Based on Multivalent Aptamer-Encoded DNA Flowers as Fluorescence Resonance Energy Transfer Platform

Qi,

Dong,

Hamed

et al. 2024

Anal. Chem.

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Life-threatening allergic reactions to food allergens, particularly peanut protein Ara h1, are a growing public health concern affecting millions of people worldwide. Thus, accurate and rapid detection is necessary for allergen labeling and dietary guidance and ultimately preventing allergic incidents. Herein, we present a novel ratiometric fluorescence aptasensor based on multivalent aptamer-encoded DNA flowers (Mul-DNFs) for the high-stability and sensitive detection of allergen Ara h1. The flower-shaped Mul-DNFs were spontaneously packaged using ultralong polymeric DNA amplicons driven by a rolling circle amplification reaction, which contains a large number of Ara h1 specific recognition units and has excellent binding properties. Furthermore, dual-color fluorescence-labeled Mul-DNFs probes were developed by hybridizing them with Cy3-and Cy5-labeled complementary DNA (cDNA) to serve as a ratiometric fluorescence aptasensor platform based on fluorescence resonance energy transfer. Benefiting from the combined merits of the extraordinary synergistic multivalent binding ability of Mul-DNFs, the excellent specificity of the aptamer, and the sensitivity of the ratiometric sensor to avoid exogenous interference. The developed ratiometric aptasensor showed excellent linearity (0.05−2000 ng mL −1 ) with a limit of detection of 0.02 ng mL −1 . Additionally, the developed ratiometric fluorescence aptasensor was utilized for quantifying the presence of Ara h1 in milk, infant milk powder, cookies, bread, and chocolate with recoveries of 95.7−106.3%. The proposed ratiometric aptasensor is expected to be a prospective universal aptasensor platform for the rapid, sensitive, and accurate determination of food and environmental hazards.

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