Lateral flow immunoassays for antigens, antibodies and haptens detection

Li, Ge; Li, Qingmei; Wang, Xiaosheng; Lei, Xiao; Zhang, Yuhang; Li, Rui; Guo, Junqing; Zhang, Gaiping

doi:10.1016/j.ijbiomac.2023.125186

Cited by 29 publications

(8 citation statements)

References 75 publications

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“…Although conventional methods for detecting PRV such as ELISA, RT-PCR, qPCR (Deblanc et (Li et al, 2011), we still chose the conventional colloidal gold in this study because colloidal gold is more stable, sensitive, and specific. The test results are visible to the naked eye and imply short time consumption, high sensitivity, good specificity, and suitability for grassroots promotion and application (Li et al, 2015(Li et al, , 2023.…”

Section: Discussionmentioning

confidence: 96%

Development and application of a high-sensitivity immunochromatographic test strip for detecting pseudorabies virus

Yin,

Liu,

Chen

et al. 2024

Front. Microbiol.

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IntroductionPseudorabies (PR) is a multi-animal comorbid disease caused by pseudorabies virus (PRV), which are naturally found in pigs. At the end of 2011, the emergence of PRV variant strains in many provinces in China had caused huge economic losses to pig farms. Rapid detection diagnosis of pigs infected with the PRV variant helps prevent outbreaks of PR. The immunochromatography test strip with colloidal gold nanoparticles is often used in clinical testing due to its low cost and high throughput.MethodsThis study was designed to produce monoclonal antibodies targeting PRV through immunization of mice using the eukaryotic system to express the gE glycoprotein. Subsequently, paired monoclonal antibodies were screened based on their sensitivity and specificity for use in the preparation of test strips.Results and discussionThe strip prepared in this study was highly specific, only PRV was detected, and there was no cross-reactivity with glycoprotein gB, glycoprotein gC, glycoprotein gD, and glycoprotein gE of herpes simplex virus and varicellazoster virus, porcine epidemic diarrhea virus, Senecavirus A, classical swine fever virus, porcine reproductive and respiratory syndrome virus, and porcine parvovirus. Moreover, it demonstrated high sensitivity with a detection limit of 1.336 × 103 copies/μL (the number of viral genome copies per microliter); the coincidence rate with the RT-PCR detection method was 96.4%. The strip developed by our laboratory provides an effective method for monitoring PRV infection and controlling of PR vaccine quality.

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Section: Discussionmentioning

confidence: 96%

Development and application of a high-sensitivity immunochromatographic test strip for detecting pseudorabies virus

Yin,

Liu,

Chen

et al. 2024

Front. Microbiol.

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“…However, these methods need specialized experimental instruments or highly skilled assayers to avoid potential operator differences. Compared with these methods, the colloidal gold immunochromatographic detection technique is simple, exhibits rapid response, and does not depend on instrumentation (Li et al 2023 ). After diluting the serum to be examined, the experimental results can be observed within 5–15 min, and the technique is more suitable for on-site testing on farms.…”

Section: Discussionmentioning

confidence: 99%

Development and application of a colloidal-gold immunochromatographic strip for detecting Getah virus antibodies

Jiang,

Qin,

Zhang

et al. 2024

Appl Microbiol Biotechnol

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Getah virus (GETV) is a re-emerging mosquito-borne alphavirus that is highly pathogenic, mainly to pigs and horses. There are no vaccines or treatments available for GETV in swine in China. Therefore, the development of a simple, rapid, specific, and sensitive serological assay for GETV antibodies is essential for the prevention and control of GETV. Current antibody monitoring methods are time-consuming, expensive, and dependent on specialized instrumentation, and these features are not conducive to rapid detection in clinical samples. To address these problem, we developed immunochromatographic test strips (ICTS) using eukaryotically expressed soluble recombinant p62-E1 protein of GETV as a labelled antigen, which has good detection sensitivity and no cross-reactivity with other common porcine virus-positive sera. The ICTS is highly compatible with IFA and ELISA and can be stored for 1 month at 37 °C and for at least 3 months at room temperature. Hence, p62-E1-based ICTS is a rapid, accurate, and convenient method for rapid on-site detection of GETV antibodies. Key points • We established a rapid antibody detection method that can monitor GETV infection • We developed colloidal gold test strips with high sensitivity and specificity • The development of colloidal gold test strips will aid in the field serologic detection of GETV

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“…Lateral flow immunoassay (LFIA) technology stands out for its low cost, instrument-free test, and simplicity, thus, making it more suitable for PSA detection in resource-limited settings. − Currently, the sensitivity of conventional Au-based LFIA is insufficient for obtaining highly reliable PSA test results. − Conventional sensitivity-enhancing designs, such as slowing down flow speed, extending the time of immune response, , and exploring self-assembly clusters, may lead to increased noise and prolonged detection times, which are unfavorable for POCT applications. To address this challenge, a high-sensitivity photothermal signal was employed to compensate for the insufficient intensity of the colorimetric signal. − Notably, the photothermal signal generated under laser irradiation demonstrates excellent anti-interference ability against changes in the solution environment, with minimal influence from biomolecules present in the solution. − On these grounds, a colorimetric signal can be combined with a photothermal signal to create a “two-in-one” modal switching LFIA, enabling reliable PSA test results within an acceptable detection time.…”

Section: Introductionmentioning

confidence: 99%

Colorimetric and Photothermal Dual-Modal Switching Lateral Flow Immunoassay Based on a Forced Dispersion Prussian Blue Nanocomposite for the Sensitive Detection of Prostate-Specific Antigen

Gong,

Gai,

Tao

et al. 2024

Anal. Chem.

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Prostate-specific antigen (PSA) is a key marker for a prostate cancer diagnosis. The low sensitivity of traditional lateral flow immunoassay (LFIA) methods makes them unsuitable for point-of-care testing. Herein, we designed a nanozyme by in situ growth of Prussian blue (PB) within the pores of dendritic mesoporous silica (DMSN). The PB was forcibly dispersed into the pores of DMSN, leading to an increase in exposed active sites. Consequently, the atom utilization is enhanced, resulting in superior peroxidase (POD)-like activity compared to that of cubic PB. Antibody-modified DMSN@PB nanozymes serve as immunological probes in an enzymatic-enhanced colorimetric and photothermal dual-signal LFIA for PSA detection. After systematic optimization, the LFIA based on DMSN@PB successfully achieves a 4-fold amplification of the colorimetric signal within 7 min through catalytic oxidation of the chromogenic substrate by POD-like activity. Moreover, DMSN@PB exhibits an excellent photothermal conversion ability under 808 nm laser irradiation. Accordingly, photothermal signals are introduced to improve the anti-interference ability and sensitivity of LFIA, exhibiting a wide linear range (1–40 ng mL–1) and a low PSA detection limit (0.202 ng mL–1), which satisfies the early detection level of prostate cancer. This research provides a more accurate and reliable visualization analysis methodology for the early diagnosis of prostate cancer.

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Lateral flow immunoassays for antigens, antibodies and haptens detection

Cited by 29 publications

References 75 publications

Development and application of a high-sensitivity immunochromatographic test strip for detecting pseudorabies virus

Development and application of a high-sensitivity immunochromatographic test strip for detecting pseudorabies virus

Development and application of a colloidal-gold immunochromatographic strip for detecting Getah virus antibodies

Colorimetric and Photothermal Dual-Modal Switching Lateral Flow Immunoassay Based on a Forced Dispersion Prussian Blue Nanocomposite for the Sensitive Detection of Prostate-Specific Antigen

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