Lateral Flow Immunochromatographic Assay for Competitive Detection of Crustacean Allergen Tropomyosin Using Phage-Displayed Shark Single-Domain Antibody

Abstract

The common food allergy crustacean tropomyosin (TM) poses a significant food safety challenge, which requires rapid and sensitive methods for screening TM in food. Herein, the variable new antigen receptor (VNAR) single-domain antibodies specific for the crustacean TM were isolated from a nai ̈ve phage-displayed shark VNAR library. Subsequently, a lateral flow immunochromatographic assay (LFIA) based on the gold nanoparticle-labeled phage-displayed shark VNAR (AuNPs@PSV) probe was developed for the detection of TM in food. The AuNPs@PSV-LFIA took 15 min for one test and had a visual limit of detection (vLOD) of 0.1 μg/mL and an instrumental LOD of 0.02 μg/mL. Good selectivity, accuracy, precision, and stability were confirmed for the AuNPs@PSV-LFIA. Moreover, the test results of 21 commercially available food products consisted of the allergen labels and were validated by a commercial ELISA kit. Therefore, this work demonstrated the great potential of VNAR for detecting TM in food by LFIA.

Rapid immunoassay for dual-mode detection of HPV16 and HPV18 DNA based on Au@PdPt nanoparticles

“…13 Owing to its advantages of simplicity in operation, speed, high sensitivity, and cost-effectiveness, LFIA has been widely used in various fields such as biomedicine, pathogen detection, drug detection, environmental monitoring, and food safety. 14–18 However, the sensitivity of traditional LFIA using Au nanoparticles (AuNPs) as visual signals is generally lower than that of LFIA based on other metal nanoparticles. 19,20 Scientists have endeavored to improve the capabilities of LFIA, such as the sensitivity, accuracy and multi-target detection capability, by introducing new materials, technologies and methods.…”