Layered Double Hydroxide Modified with Deoxycholic and Hyaluronic Acids for Efficient Oral Insulin Absorption

Huang, Xia; Han, Shangcong; Chen, Zuxian; Zhao, Lei; Wang, Changduo; Guo, Qingyang; Li, Yanfeng; Sun, Yong

doi:10.2147/ijn.s323381

Cited by 9 publications

(1 citation statement)

References 27 publications

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“…Fluids are more easily diffused and more quickly absorbed into the general circulation [13]. This action of HYAL has been widely used to accelerate the diffusion and absorption of subcutaneously injected fluids and drugs [16,17]. However, only limited studies have investigated the potential of HYAL as a therapeutic agent preventing lymphedema [15].…”

Section: Introductionmentioning

confidence: 99%

Short term treatment of secondary lymphedema with hyaluronidase injections reduces mouse hindlimb lymphedema

Dalaei

Bučan

Wiinholt

et al. 2023

JPHS

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Lymphedema is a common complication following breast cancer treatment with axillary lymphadenectomy and radiotherapy. Currently, there is no curative treatment for this disease, hence there is a need for new therapeutic suggestions. The aim of this study was to investigate the effect of hyaluronidase (HYAL) injections after inducing hindlimb lymphedema in 36 female C57BL/6 mice. HYAL injections were administered every second day for 14 days in three groups: (1) HYAL for 1 week followed by saline for 1 week, (2) HYAL for 2 weeks, and (3) saline injections for 2 weeks. Volume of the lymphedema limb was weekly assessed with micro-computed tomography (μ-CT) scans for a total course of 6 weeks. Lymph vessel morphometry was assessed in the end of the study after staining cross-sections of the hindlimb for anti-LYVE-1 blindly. Lymphatic function was assessed by lymphoscintigraphy to assess lymphatic clearance. There was a significant reduction of the volume of lymphedema in mice treated with HYAL-7 compared with mice treated with HYAL-14 (p < 0.05) and saline (p < 0.05). No differences were detected in lymph vessel morphometry and the lymphoscintigraphy between groups. Short-term treatment with HYAL-7 might be a potential therapeutic suggestion for secondary lymphedema induced in mouse hindlimbs. In the future, clinical studies are needed to investigate the potential of HYAL treatment in human beings.

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Section: Introductionmentioning

confidence: 99%

Short term treatment of secondary lymphedema with hyaluronidase injections reduces mouse hindlimb lymphedema

Dalaei

Bučan

Wiinholt

et al. 2023

JPHS

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Novel pH‐sensitive gellan gum–ε‐polylysine hydrogel microspheres for sulforaphene delivery

Qu,

Li,

Chen

et al. 2024

J Sci Food Agric

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BACKGROUNDThis study aimed to improve the stability and utilization of sulforaphene (SFE) and to enhance the intestinal stability and pH‐sensitive release of SFE in the gastrointestinal tract. To achieve this objective, calcium chloride (CaCl2) was used as a crosslinking agent to fabricate novel SFE‐loaded gellan gum (GG)‐ε‐polylysine (ε‐PL) pH‐sensitive hydrogel microspheres by using the ionic crosslinking technique.RESULTSThe molecular docking results of GG, ε‐PL, and SFE were good and occurred in the natural state. The loading efficiency (LE) of all samples was above 70%. According to the structural characterization results, GG and ε‐PL successfully embedded SFE in a three‐dimensional network structure through electrostatic interaction. The swelling characteristics and in vitro release results revealed that the microspheres were pH‐sensitive, and SFE was mainly retained inside the hydrogel microsphere in the stomach, and subsequently released in the intestine. The result of cytotoxicity assay showed that the hydrogel microspheres were non‐toxic and had an inhibitory effect on human colon cancer Caco‐2 cells.CONCLUSIONThus, the hydrogel microspheres could improve SFE stability and utilization and achieve the intestinal targeted delivery of SFE. © 2024 Society of Chemical Industry.

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Development of polysaccharide-coated layered double hydroxide nanocomposites for enhanced oral insulin delivery

Pang,

Wu,

Chen

et al. 2024

Drug Deliv. and Transl. Res.

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Oral insulin (INS) is predicted to have the most therapeutic advantages in treating diabetes to repress hepatic glucose production through its potential to mimic the endogenous insulin pathway. Many oral insulin delivery systems have been investigated. Layered double hydroxide (LDH) as an inorganic material has been widely used in drug delivery thanks to its appealing features such as good biocompatibility, low toxicity, and excellent loading capability. However, when used in oral drug delivery, the effectiveness of LDH is limited due to the acidic degradation in the stomach. In this study, to overcome these challenges, chitosan (Chi) and alginate (Alg) dual-coated LDH nanocomposites with the loading of insulin (Alg-Chi-LDH@INS) were developed by the layered-by-layered method for oral insulin delivery with dynamic size of ~ 350.8 nm, negative charge of ~ − 13.0 mV, and dispersity index 0.228. The insulin release profile was evaluated by ultraviolet–visible spectroscopy. The drug release profiles evidenced that alginate and chitosan coating partially protect insulin release from a burst release in acidic conditions. The analysis using flow cytometry showed that chitosan coating significantly enhanced the uptake of LDH@INS by Caco-2 cells compared to unmodified LDH and free insulin. Further in the in vivo study in streptozocin-induced diabetic mice, a significant hypoglycemic effect was maintained following oral administration with great biocompatibility (~ 50% blood glucose level reduction at 4 h). This research has thus provided a potential nanocomposite system for oral delivery of insulin. Graphical Abstract

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Layered Double Hydroxide Modified with Deoxycholic and Hyaluronic Acids for Efficient Oral Insulin Absorption

Cited by 9 publications

References 27 publications

Short term treatment of secondary lymphedema with hyaluronidase injections reduces mouse hindlimb lymphedema

Short term treatment of secondary lymphedema with hyaluronidase injections reduces mouse hindlimb lymphedema

Novel pH‐sensitive gellan gum–ε‐polylysine hydrogel microspheres for sulforaphene delivery

Development of polysaccharide-coated layered double hydroxide nanocomposites for enhanced oral insulin delivery

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