Layered Double Hydroxide Nanotransporter for Molecule Delivery to Intact Plant Cells

Abstract

Here we report a powerful method that facilitates the transport of biologically active materials across the cell wall barrier in plant cells. Positively charged delaminated layered double hydroxide lactate nanosheets (LDH-lactate-NS) with a 0.5‒2 nm thickness and 30‒60 nm diameter exhibit a high adsorptive capacity for negatively charged biomolecules, including fluorescent dyes such as tetramethyl rhodamine isothiocyanate (TRITC), fluorescein isothiocyanate isomer I(FITC) and DNA molecules, forming neutral LDH-nanosheet conjugates. These neutral conjugates can shuttle the bound fluorescent dye into the cytosol of intact plant cell very efficiently. Furthermore, typical inhibitors of endocytosis and low temperature incubation did not prevent LDH-lactate-NS internalization, suggesting that LDH-lactate-NS penetrated the plasma membrane via non-endocytic pathways, which will widen the applicability to a variety of plant cells. Moreover, the absence of unwanted side effects in our cytological studies, and the nuclear localization of ssDNA-FITC suggest that nano-LDHs have potential application as a novel gene carrier to plants.

Interactions between Biological Cells and Layered Double Hydroxides: Towards Functional Materials

“…Moreover, the ability of LDH nanoplatelets to escape the endosomes once uptake promotes a high efficiency of gene and drug delivery ,. Internalization of LDH was also evidenced for plant cells without any damage for the cells …”

Osmotic stress‐responsive promoter upstream transcripts (PROMPTs) act as carriers of MYB transcription factors to induce the expression of target genes in Populus simonii

“…Candidate lncRNAs were artificially synthesized (Table S8). These lncRNAs were dissolved in distilled water to a concentration of 1 mg/mL (Bao et al ., ). The LDH‐lactate‐NS colloid in MS was added dropwise to the lncRNAs at a ratio of 3 : 1 (v:v) followed by gentle mixing.…”

“…Candidate lncRNAs were artificially synthesized (Table S8). These lncRNAs were dissolved in distilled water to a concentration of 1 mg/mL (Bao et al, 2016).…”

Porous Inorganic and Hybrid Systems for Drug Delivery: Future Promise in Combatting Drug Resistance and Translation to Botanical Applications

“…180 The immobilization of biomolecules of interest inbetween the platelets is a way to transport them through cell walls with minimal damage, without loss of efficiency, and without unspecific reaction to non-targeted tissues. 181 Both LDH and halloysites have also been used to control the release of an herbicide, showing similar release profiles and efficiency. 156 Overall, the pharmaceutical developments of the last decades are a significant source of inspiration for agro-industrial delivery applications.…”