LB broth-lyophilized Rabbit serum (LLR) as a new and suitable culture medium for cultivation of promastigotes of Leishmania major

Nasiri, Vahid; Dalimi, Abdolhossein; Ghaffarifar, Fatemeh

doi:10.1007/s12639-016-0786-1

Cited by 6 publications

(4 citation statements)

References 22 publications

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“…A 0.1 ml of the suspension was plated on Luria broth medium which contains the following composition (g/L dist. H2O): yeast extract, 5 g; peptone,10 g and NaCl, 10 g (Nasiri et al 2017). The plates were incubated at 37°C overnight, single colonies were re-streaked onto fresh plates.…”

Section: Isolation Of Microorganisms From Molasses and Vinassesmentioning

confidence: 99%

Enhancement of sustainable bioethanol production from microorganisms isolated from molasses and venasses

Mostafa,

Abdel-Fattah,

El-Rawy

et al. 2024

Egyptian Sugar Journal

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Ethanol is one of the most important biofuels that can be produced from different renewable sources. Molasses and Venasses are used as cheap raw materials in the isolation of microorganisms and used molasses as renewable materials for ethanol production. Molasses and Venasses are considered important by-products in the sugar industry. This study aims to isolate and identify yeasts and bacteria present in both molasses and venasses to use them in the production of bioethanol. Molasses and venasses samples were collected from ten different sugar factories (Guirga, Savola, Deshna, Komombo, Abokorkaus, Delta, Dakahlia, Qus, Nag-hamdy, Armant) and were used to isolate different microorganisms that were screened for their bioethanol productivity. The results showed that the molasses samples contained more microbes than venasses. Twelve isolates were molecularly identified as S. cerevisiae by PCRspecific primers, while 64 isolates were bacterial isolates. All the yeast and bacterial isolates were screened for bioethanol productivity. Isolate M3 showed the highest bioethanol productivity (74%) and was identified by 16S rRNA gene sequencing as Klebsiella pneumoniae. Several factors affected the production of bioethanol, including sugar concentration, urea, and ammonium sulfate. When molasses was used as the carbon source, Klebsiella pneumoniae produced 1% (v/v) bioethanol by utilizing 20% molasses (sugar concentration), 0.4% urea, and 0.4% ammonium sulfate. When UV-mutagenesis was used to improve the bioethanol productivity, all the obtained mutants showed lower productivity compared to the wild-type (M3 isolate).

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Section: Isolation Of Microorganisms From Molasses and Vinassesmentioning

confidence: 99%

Enhancement of sustainable bioethanol production from microorganisms isolated from molasses and venasses

Mostafa,

Abdel-Fattah,

El-Rawy

et al. 2024

Egyptian Sugar Journal

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“…Luria–Bertani (LB) broth medium is a complex medium known to promote the structural evolution of subpopulations, mainly containing tryptone, yeast extract and NaCl [ 1 ]. LB broth medium can be divided into liquid medium and agar medium.…”

Section: Introductionmentioning

confidence: 99%

The Metabolomics Changes in Luria–Bertani Broth Medium under Different Sterilization Methods and Their Effects on Bacillus Growth

Wang,

Guo,

Chen

et al. 2023

Metabolites

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Luria–Bertani broth (LB) culture medium is a commonly used bacterial culture medium in the laboratory. The nutrient composition, concentration, and culture conditions of LB medium can influence the growth of microbial strains. The purpose of this article is to demonstrate the impact of LB liquid culture medium on microbial growth under different sterilization conditions. In this study, LB medium with four different treatments was used, as follows: A, LB medium without treatments; B, LB medium with filtration; C, LB medium with autoclaving; and D, LB medium with autoclaving and cultured for 12 h. Subsequently, the protein levels and antioxidant capacity of the medium with different treatments were measured, and the effects of the different LB medium treatments on the growth of microorganisms and metabolites were determined via 16s rRNA gene sequencing and metabolomics analysis, respectively. Firmicutes and Lactobacillus were the dominant microorganisms, which were enriched in fermentation and chemoheterotrophy. The protein levels and antioxidant capacity of the LB medium with different treatments were different, and with the increasing concentration of medium, the protein levels were gradually increased, while the antioxidant capacity was decreased firstly and then increased. The growth trend of Bacillus subtilis, Bacillus paralicheniformis, Micrococcus luteus, and Alternaria alternata in the medium with different treatments was similar. Additionally, 220 and 114 differential metabolites were found between B and C medium, and between C and D medium, which were significantly enriched in the “Hedgehog signaling pathway”, “biosynthesis of plant secondary metabolites”, “ABC transporters”, “arginine and proline metabolism”, and “linoleic acid metabolism”. LB medium may be a good energy source for Lactobacillus growth with unsterilized medium, and LB medium filtered with a 0.22 μm filter membrane may be used for bacterial culture better than culture medium after high-pressure sterilization. LB medium still has the ability for antioxidation and to keep bacteria growth whether or not autoclaved, indicating that there are some substances that can resist a high temperature and pressure and still maintain their functions.

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“…FBS is the most expensive part of this medium and increases the cost of production. Recently, Nasiri et al ( 17 ) introduced the use of lyophilized rabbit serum in culture media instead of FBS. Lyophilized rabbit serum is less expensive than FBS and Nasiri et al have successfully used it for the cultivation of L. major ( 17 ).…”

Section: Introductionmentioning

confidence: 99%

The Influence of Different Culture Media on the Growth and Recombinant Protein Production of Iranian Lizard Leishmania Promastigote

Ghavidel¹,

Aghamiri²,

Jajarmi³

et al. 2022

IJPA

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Background: Leishmania is a eukaryotic protozoan parasite belonging to the Trypanosomatidae family. The Iranian Lizard Leishmania (I.L.L.), which is non-pathogenic to mammals, shows great promise to be used as an expression system for recombinant protein production. Unlike other Leishmania strains, the ideal culture medium for I.L.L. has not been established, although it is commonly cultured in the RPMI1640 medium. Methods: We investigated the growth rate of the wild and recombinant I.L.L. in BHI, RPMI1640, LB, and M199 media with and without FBS, hemin, or lyophilized rabbit serum. Subsequently, the expression rate of the recombinant protein in these media was compared. Results: The growth rate of I.L.L. in RPMI1640 medium and LB broth was similar and supplementation with 10% FBS did not affect the growth rate. The amount of protein expression in the LB medium was higher than in the other three media. Conclusion: The LB broth is an appropriate medium for I.L.L. culture and recombinant protein production.

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LB broth-lyophilized Rabbit serum (LLR) as a new and suitable culture medium for cultivation of promastigotes of Leishmania major

Cited by 6 publications

References 22 publications

Enhancement of sustainable bioethanol production from microorganisms isolated from molasses and venasses

Enhancement of sustainable bioethanol production from microorganisms isolated from molasses and venasses

The Metabolomics Changes in Luria–Bertani Broth Medium under Different Sterilization Methods and Their Effects on Bacillus Growth

The Influence of Different Culture Media on the Growth and Recombinant Protein Production of Iranian Lizard Leishmania Promastigote

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