LBH589 Promotes Osteogenic and Dentinogenic Differentiation of Stem Cells from the Apical Papilla by Inhibiting Histone Deacetylation

Abstract

To investigate the effects of the histone deacetylase inhibitor LBH589 on the osteogenic differentiation of stem cells from the apical papilla (SCAPs) in vitro. Mesenchymal stem cells were isolated from the apical papilla with patient consent. MTT assays and flow cytometric analysis of annexin V were performed to evaluate the effect of LBH589 on the proliferation and apoptosis of the SCAPs. SCAPs were cultured in osteogenic conditioned alpha minimum essential medium ( -MEM). After stimulation with different concentrations of LBH589, alkaline phosphatase staining, alkaline phosphatase activity analysis, and alizarin red staining were performed to detect the osteogenic effects of LBH589. In our in vitro study, cells treated with LBH589 showed significantly enhanced alkaline phosphatase staining, alkaline phosphatase activity, and calcium nodule formation. This study demonstrated that, as a global histone deacetylase inhibitor, LBH589 has the potential to promote the osteogenic and dentinogenic differentiation of stem cells from the apical papilla.