LBX2-AS1 up-regulated by NFIC boosts cell proliferation, migration and invasion in gastric cancer through targeting miR-491-5p/ZNF703

Abstract

Background: The crucial role of long non-coding RNAs (lncRNAs) has been certified in human cancers. The lncRNAs with abnormal expressions could act as tumor inhibitors or oncogenes in the advancement of tumors. LBX2-AS1 was once reported to accelerate esophageal squamous cell carcinoma. Nonetheless, its function in gastric cancer (GC) remained a riddle. Methods: RT-qPCR was used to examine the expression of NFIC/LBX2-AS1/miR-491-5p/ZNF703 in GC cell lines. The functions of LBX2-AS1 in GC were appraised by colony formation, EdU, flow cytometry analysis, transwell and wound healing assays. Luciferase reporter, ChIP and RNA pull down assays were utilized to evaluate the interactions among genes. Results: LBX2-AS1 was up-regulated in GC cell lines. Knockdown of LBX2-AS1 repressed the proliferative, migratory, and invasive abilities of GC cells. Moreover, LBX2-AS1 was transcriptionally activated by NFIC. And LBX2-AS1 could bind with miR-491-5p. Besides, miR-491-5p depletion or ZNF703 upregulation could counteract the repressing effects of LBX2-AS1 silence on GC progression. Conclusion: In a word, LBX2-AS1 up-regulated by NFIC promoted GC progression via targeting miR-491-5p/ZNF703, implying LBX2-AS1 was an underlying treatment target for GC patients.

MiR-491-5p, as a Tumor Suppressor, Prevents Migration and Invasion of Breast Cancer by Targeting ZNF-703 to Regulate AKT/mTOR Pathway

“…Further clarification of the mechanism and role of different miRNAs in BC is of great significance for diagnosis and targeted therapy. MiR-491-5p, as a member of the miRNA family with regulatory oncogenes, can prevent the processes of multiple cancers, including bladder cancer, 14 non-Small cell lung cancer, 15 lung adenocarcinoma, 16 acute lymphoblastic leukemia, 17 gastric cancer, 18 and so on. However, the role and mechanism of miR-491-5p in BC progression are completely unclear.…”

Overexpression of sperm associated antigen 5 promotes cell growth, metastasis, and adriamycin resistance in esophageal squamous cell carcinoma via activating PI3K/AKT signaling pathway

“…On the contrary, overexpression of SPAG5 gene significantly promoted the viability, proliferation, migration, invasion and inhibition of apoptosis of ESCC cells (Figure 4 and Figure 5). It is reported that cell proliferation, apoptosis, migration and invasion are closely related to the changes of the related protein expression [11,15,16,19,24]. The expression of Caspase-3, Ki67 and VEGF in tumor tissues is closely related to the proliferation and apoptosis.…”

“…The proliferation, apoptosis, migration and invasion of tumor cells are the necessary stages of tumor occurrence and development [2,11,15,16,19]. Drug resistance is one of the important reasons for the low survival rate of cancer patients [21].…”

“…The abnormal cell proliferation is an important mechanism of tumorigenesis [11]. In the process of cell mitosis, the abnormal structure and function of spindle and centromere are important factors for the abnormal cell proliferation.…”

Role of microRNAs in Pressure Ulcer Immune Response, Pathogenesis, and Treatment

“…The inhibition of MMP9 by miR-491-5p has been implicated in lowering metastasis and tissue invasion in hepatocellular carcinoma and osteosarcoma [129,130]. MiR-491-5p also decreases cellular proliferation and motility in neoplastic models through activity on other gene targets, but further investigation of its effects on MMP activity during impaired wound healing is required [131,132]. Within placental tissue, miR-491-5p inhibits MMP9 activity, leading to lower trophoblast invasion and higher rates of pre-eclampsia [133].…”