LC-APCI-MS method for detection and analysis of tryptanthrin, indigo, and indirubin in Daqingye and Banlangen

Liau, Bing-Chung; Jong, Ting‐Ting; Lee, Maw-Rong; Chen, Shih-Shiung

doi:10.1016/j.jpba.2006.06.029

Cited by 90 publications

(63 citation statements)

References 12 publications

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“…Indirubin results were similar or lower than those in the previous studies (Liau et al 2007;Gilbert et al 2004;Zou and Koh 2007;Chen et al 2011). Indigo content (2.80 mg/ g) was previously reported by our research group (Karaman et al 2016).…”

Section: Resultssupporting

confidence: 89%

“…In natural Isatis plants, some indigo precursors and indirubin known as red isomer of indigo were also tested for their genotoxicity (Candido-Bacani et al 2011) and indirubin and its derivatives were reported to possess low toxicity (Zhen et al 2007). Various biological activities including antifebrile, anti-inflammatory, antiviral, antimicrobial, detoxifying purposes, restenosis and skin psoriasis (Hoessel et al 1999;Chiang et al 2013;Liau et al 2007) and antileukemic activity against myelocytic leukemia in Traditional Chinese Medicine (Hoessel et al 1999) have been reported for indirubin and its derivatives (Hoessel et al 1999). Studies with respect to the monitoring the possible toxic effects of plant species are of great concern for public and environmental health for desired sustainable life comfort.…”

Section: Introductionmentioning

confidence: 99%

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The role of natural indigo dye in alleviation of genotoxicity of sodium dithionite as a reducing agent

et al. 2016

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Indigo blue is a natural dye used for thousands of years by civilizations to dye fabric blue and it is naturally obtained from Isatis tinctoria. I. tinctoria is not only used for extraction of indigo blue color but also used medicinally in Traditional Chinese Medicine because of its active compounds. Sodium dithionite (Na 2 S 2 O 4 ) is used in dye bath for indigo blue extraction, but this reducing agent and its derivatives are major pollutants of textile industry and subsequently have hazardous influences on public health. Herein, the present study was designed to obtain the high yield of natural indigo dye but with low possible toxic effect. In this context, genotoxic effects of particular combinations of natural dye solutions obtained from Isatis tinctoria subsp. tomentolla with Na 2 S 2 O 4 as reducing agent were investigated. Dye solutions were obtained using two different pH levels (pH 9 and 11) and three different concentrations of Na 2 S 2 O 4 (2.5, 5 and 10 mg/ml). In addition to the dye solutions and reducing agent, aqueous extracts of I. tinctoria were assessed for their genotoxicity on human lymphocytes. For in vitro testing of genotoxicity, chromosomal aberrations (CAs), sister chromatid exchanges (SCEs) and mitotic indexes (MI) assays were used. Accordingly, Na 2 S 2 O 4 caused significant increases in CA and SCE as well decrease in MI but the genotoxic effects of sodium dithionite were reduced with natural indigo dye. As a result, aqueous extracts of Isatis leaves removed the toxic effects of sodium dithionite and showed anti-genotoxic effect. For the optimal and desired quality but with less toxic effects of natural dye, 2.5 mg/ml (for wool yarn) and 5 mg/ml (for cotton yarn) of Na 2 S 2 O 4 doses were found to be the best doses for reduction in the dye bath at Ph 9.

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Section: Resultssupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

The role of natural indigo dye in alleviation of genotoxicity of sodium dithionite as a reducing agent

et al. 2016

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“…TLC plate는 aluminum plate에 Silica gel 60 F254 가 도포된 것을 사용하였다(Merck, Darmstatt, Germany). 전개액은 chloroform과 acetonitrile을 8.5: (Liau et al, 2007), 발효 과정에서 인디칸의 가수분 해가 완전히 이루어졌는지의 여부 (Angelini et al, 2004;Gilbert et al, 2004;Stoker et al, 1998;Torimoto, 1987) …”

Section: ) Tlc(thin Layer Chromatography) 분석unclassified

Analysis of the Pigment Contents of Commercial Indigo Powders and Their Effect on the Color and the Antimicrobial Function of Dyed Cotton Fabrics

Ahn²

2013

Journal of the Korean Society of Clothing and Textiles

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Market available fermented indigo powders of Indian origin (FI1, FI2), Chinese origin (FC1, FC2), and raw indigo powders of Indian origin (R1, R2) were examined using TLC and HPLC analyses to investigate their pigment contents. TLC analysis gave Rf values of 0.81 and 0.72 for blue and red pigments, respectively. All the powder products and the synthetic and natural indigo standards eluted at 6.9 min and 8.3 min in the HPLC chromatograms, and the peaks showed the λmax at 610nm and 542nm, representing indigotin and indirubin, respectively. The pigment content calculated based on the area of indigotin and indirubin peaks in the HPLC chromatograms showed that the indigotin content was higher in FC1 and FC2, while FI1 and FI2 had a higher indirubin content. The relative percentage of indirubin was the highest in R2, but the HPLC peak intensity was quite low. Despite the higher indigotin content in FC1 and FC2, cotton dyed with FI1 and FI2 (versus cotton dyed with FC1 and FC2) showed a higher blue (B) hue, the highest K/S values, and the highest antimicrobial effect.

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“…Immediately before extraction, dried leaf material was grounded. 1.0 g frozen and powdered samples was extracted by Pressurised Liquid Extraction (PLE) with an ASE 200 instrument (Liau et al, 2007). Bagci and Özçelik (2009) investigated fatty acid in the seeds of Isatis tinctoria using GC and HPLC.…”

Section: Extraction and Separationmentioning

confidence: 99%

Analysis of the Extracts of <i>Isatis tinctoria</i> by New Analytical Approaches of HPLC, MS and NMR

Zhou¹,

Qu²

2011

Afr. J. Trad. Compl. Alt. Med.

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The methods of extraction, separation and analysis of alkaloids and indole glucosinolates (GLs) of Isatis tinctoria were reviewed. Different analytical approaches such as High-pressure Liquid Chromatography (HPLC), Liquid Chromatography with Electrospray Ionization Mass Spectrometry (LC/ESI/MS), Electrospray Ionization Time-Of-FlightMass Spectrometry (ESI-TOF-MS), and Nuclear Magnetic Resonance (NMR) were used to validate and identity of these constituents. These methods provide rapid separation, identification and quantitative measurements of alkaloids and GLs of Isatis tinctoria. By connection with different detectors to HPLC such as PDA, ELSD, ESI-and APCI-MS in positive and negative ion modes, complicated compounds could be detected with at least two independent detection modes. The molecular formula can be derived in a second step of ESI-TOF-MS data. But for some constituents, UV and MS cannot provide sufficient structure identification. After peak purification, NMR by semi-preparative HPLC can be used as a complementary method.

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LC-APCI-MS method for detection and analysis of tryptanthrin, indigo, and indirubin in Daqingye and Banlangen

Cited by 90 publications

References 12 publications

The role of natural indigo dye in alleviation of genotoxicity of sodium dithionite as a reducing agent

The role of natural indigo dye in alleviation of genotoxicity of sodium dithionite as a reducing agent

Analysis of the Pigment Contents of Commercial Indigo Powders and Their Effect on the Color and the Antimicrobial Function of Dyed Cotton Fabrics

Analysis of the Extracts of <i>Isatis tinctoria</i> by New Analytical Approaches of HPLC, MS and NMR

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