LC coupled to ESI, MALDI and ICP MS – A multiple hyphenation for metalloproteomic studies

Coufalíková, Kateřina; Benešová, Iva; Vaculovič, Tomáš; Kanický, Viktor; Preisler, Jan

doi:10.1016/j.aca.2017.03.016

Cited by 13 publications

(6 citation statements)

References 47 publications

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“…However, the metamorphic nature of the protein with structures depending on metal load and oxidation state and different structures of Zn 4–6 MT and high coordination dynamics of Zn 2+ ions in the protein may prevent the development of such antibodies [110,111]. Another possibility are mass spectrometry techniques with combined elemental and molecular resolution [26,112,113]. …”

Section: Metallothionein: a Metamorphic Protein With A Structure Dmentioning

confidence: 99%

The Functions of Metamorphic Metallothioneins in Zinc and Copper Metabolism

Krężel

Maret

2017

IJMS

235

143

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Recent discoveries in zinc biology provide a new platform for discussing the primary physiological functions of mammalian metallothioneins (MTs) and their exquisite zinc-dependent regulation. It is now understood that the control of cellular zinc homeostasis includes buffering of Zn2+ ions at picomolar concentrations, extensive subcellular re-distribution of Zn2+, the loading of exocytotic vesicles with zinc species, and the control of Zn2+ ion signalling. In parallel, characteristic features of human MTs became known: their graded affinities for Zn2+ and the redox activity of their thiolate coordination environments. Unlike the single species that structural models of mammalian MTs describe with a set of seven divalent or eight to twelve monovalent metal ions, MTs are metamorphic. In vivo, they exist as many species differing in redox state and load with different metal ions. The functions of mammalian MTs should no longer be considered elusive or enigmatic because it is now evident that the reactivity and coordination dynamics of MTs with Zn2+ and Cu+ match the biological requirements for controlling—binding and delivering—these cellular metal ions, thus completing a 60-year search for their functions. MT represents a unique biological principle for buffering the most competitive essential metal ions Zn2+ and Cu+. How this knowledge translates to the function of other families of MTs awaits further insights into the specifics of how their properties relate to zinc and copper metabolism in other organisms.

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Section: Metallothionein: a Metamorphic Protein With A Structure Dmentioning

confidence: 99%

The Functions of Metamorphic Metallothioneins in Zinc and Copper Metabolism

Krężel

Maret

2017

IJMS

235

143

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“…Taking into consideration all of the previous discussion, it would seem that an alternative workflow may be proposed, whereby IHC, LC-MS, SALD ICP MS and MALDI MSI are used as complementary techniques [47,48]. This more objective approach may notably decrease the timescale for biomarker discovery studies.…”

Section: Discussionmentioning

confidence: 99%

Mass spectrometric imaging of cysteine rich proteins in human skin

Vaníčková

Guráň

Kollár

et al. 2019

International Journal of Biological Macromolecules

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Looking insight pathological processes, metallothioneins (MTs) are considered to be potential biomarkers for monitoring of a development of various types of malignant disease, such as cancer. The early identification of the MT biomarkers in biological tissues could be important tool for the estimation of appropriate clinical therapy. Therefore here we investigated the application of matrix assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) together with immunohistochemical analyses (IHC) using MT-1/2 antibody for MT detection in formalin-fixed paraffin-embedded (FFPE) biopsy specimens of human skin.Principal component analyses revealed differences in the peptide/protein profiles separating healthy skin from the carcinoma specimens. Statistically significant ion peaks at m/z 6038, 6300, 6676, and 7026 were more frequently detected in squamous cell carcinoma (SCC), basal cell carcinoma (BCC) and melanoma. Using IHC, we found that MT-1/2 was significantly higher in SCC and melanoma compared to healthy skin. Surprisingly, significantly low levels of MT-1/2 were found in BCC. On one side, the results indicate important role of MTs in melanoma occurrence and progression, as on the second side, there are hidden processes associated with MTs based on differences of the occurrence of the MS peaks, which could be associated with cycling of MTs isoforms.

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“…35 Also, the power of mass spectrometry in both molecular and elemental arrangements reported detection of MT. 22,36 The combination of these two principally different techniques is a very powerful and elegant approach to the investigation of important biomolecules. Moreover, the exploitation of the selective extraction strategy may signicantly improve the overall sensitivity.…”

Section: Mag-mip-based Extraction Of Mtmentioning

confidence: 99%