LC/ESI-MS method for the determination of trimetazidine in human plasma: Application to a bioequivalence study on Chinese volunteers

Jiao, Yang; Su, Mingming; Chen, Minjun; Wang, Jia; Chou, Yiqun; Hu, Zhongyi; Yang, Nanlin; Tong, Weida

doi:10.1016/j.jpba.2006.11.040

Cited by 25 publications

(33 citation statements)

References 8 publications

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“…The mechanism of action is based on changing the energy substrate preference from fatty acid oxidation to glucose oxidation which adjusts the cellular energy processes, and in the main time it maintains appropriate energy metabolism during ischemia condition. It has no effect on the myocardial oxygen consumption or the coronary blood flow [6]. …”

Section: Introductionmentioning

confidence: 99%

“…Different analytical methods have been applied for the determination of atenolol in biological fluids and pharmaceutical products such as HPLC [3, 11], LC-MS [5, 6], GC-MS [12], TLC [13], spectrophotometric and spectrofluorimetric methods [14–16], titrimetry [17], voltammetry [18], chemiluminescence [19], and capillary electrophoresis [20]. Several clean-up methods such as liquid-liquid extraction [3, 11, 12], solid phase extraction [21], and protein precipitation [22] have been used for extracting ATN from biological fluid samples.…”

Section: Introductionmentioning

confidence: 99%

“…TMZ has been isolated from biological fluids using liquid-liquid extraction [5], solid phase extraction [35], and protein precipitation methods [6]. …”

Section: Introductionmentioning

confidence: 99%

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Determination of Atenolol and Trimetazidine in Pharmaceutical Tablets and Human Urine Using a High Performance Liquid Chromatography-Photo Diode Array Detection Method

El-Alfy

Ismaiel

El-Mammli

et al. 2019

International Journal of Analytical Chemistry

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A simple RP-HPLC-PDA method for determination of atenolol (ATN) and trimetazidine (TMZ) in human urine and tablets has been developed. Analytes were separated on a Caltrex BI column (125× 4.0 mm, 5 μm) with 25mM potassium dihydrogen phosphate pH 3.3, methanol, and acetonitrile mobile phases. The PDA detector was operated at 210 nm for TMZ and 225 nm for ATN and the flow rate was 1.0 mL/ min. Linearity was obtained over a concentration range of (1.0-100 μg/mL) for both analytes in standard solutions and the method was successfully applied for determination of target analytes in their pharmaceutical tablets. Excellent linearity was also obtained over concentration ranges of (0.25-25 μg/mL) and (0.5-25 μg/mL) in human urine for TMZ and ATN, respectively. A simple liquid-liquid extraction was applied for urine sample clean-up and a gradient method was used for chromatographic separation. The lower limit of quantitation (LOQ) was 0.99 and 0.60 μg/mL for ATN and TMZ, respectively. The limit of detection (LOD) was 0.30 and 0.18 μg/mL for ATN and TMZ, respectively. Inter- and intraday precision and accuracy for ATN were within ±1.89% in pure form and within ±2.85% in urine samples. Inter- and intraday precision and accuracy for TMZ were within ± 3.99% in pure form and within ± 3.19% in urine samples.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Determination of Atenolol and Trimetazidine in Pharmaceutical Tablets and Human Urine Using a High Performance Liquid Chromatography-Photo Diode Array Detection Method

El-Alfy

Ismaiel

El-Mammli

et al. 2019

International Journal of Analytical Chemistry

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“…12 Several methods are described in the literature for the quantitative analysis of Trimetazidine in serum. [13][14][15] Some authors reported the use of fluorescence detector 7 ; while others reported the use of mass spectroscopy 13,14 which is not available in all laboratories for routine analysis. Hence, the aims of the present study were to develop and validate a simple and rapid RP-HPLC method for the determination of trimetazidine in human serum samples with good resolution having the desired sensitivity when applying to a pharmacokinetic study of trimetazidine in healthy Bangladeshi male volunteers.…”

Section: Introductionmentioning

confidence: 99%

Validation and Optimization of a Simple RP-HPLC Method for Determination of Trimetazidine in Human Serum and its Application in a Pharmacokinetic Study with Healthy Bangladeshi Male Volunteers

Chowdhury

Ullah

Maruf

et al. 2012

Dhaka Univ. J. Pharm. Sci

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Abstract:Trimetazidine is an effective and well-tolerated antianginal drug. In the present study, a simple, sensitive and specific liquid chromatography (HPLC) method with UV detection was developed and validated for the quantification of trimetazidine in human serum samples using caffeine as internal standard. Protein precipitation method with methanol was employed in the extraction of trimetazidine and caffeine from biological matrix. The chromatographic separation was accomplished on Xterra C 18 Column with a mobile phase consisting 0.01 M potassium dihydrogen phosphate buffer (pH 4.16 ± 0.01 adjusted with orthophosphoric acid, with a solvent system of triethanolamine and acetonitrile (90:10) at a flow rate of 1.0 ml/min. The chromatogram was monitored at a wavelength of 207 nm. The method was validated over a linear concentration range of 5-200 ng/ml and limit of quantification (LOQ) was 5.0 ng/ml with a coefficient of correlation (r 2 ) ≥ 0.996. The intra-day and inter-day precision expressed as relative standard deviation was 3.40%-11.63% and 1.30%-10.21%, respectively. The average recovery of trimetazidine from serum was 97.44%. The method was successfully applied to a pharmacokinetic study after oral administration of modified release trimetazidine hydrochloride tablet (35 mg) in healthy Bangladeshi volunteers.

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“…19 Mean plasma concentration-time curve for trimetazidine after oral administration of 20 mg trimetazidine as test and reference formulations in 19 healthy volunteers. Reproduced with permission from Ref [264],. ; test: *.…”

mentioning

confidence: 99%

The Role of Analytical Chemistry in the Safety of Drug Therapy

Gǒrög

2012

Analytical Techniques for Clinical Chemistry

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LC/ESI-MS method for the determination of trimetazidine in human plasma: Application to a bioequivalence study on Chinese volunteers

Cited by 25 publications

References 8 publications

Determination of Atenolol and Trimetazidine in Pharmaceutical Tablets and Human Urine Using a High Performance Liquid Chromatography-Photo Diode Array Detection Method

Determination of Atenolol and Trimetazidine in Pharmaceutical Tablets and Human Urine Using a High Performance Liquid Chromatography-Photo Diode Array Detection Method

Validation and Optimization of a Simple RP-HPLC Method for Determination of Trimetazidine in Human Serum and its Application in a Pharmacokinetic Study with Healthy Bangladeshi Male Volunteers

The Role of Analytical Chemistry in the Safety of Drug Therapy

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