LC-HRMS analysis of corn mint <i>Mentha arvensis</i> L. for anticancer activity against triple-negative breast cancer targeting inflammatory and apoptosis signaling pathways

Pratyusha, Beesetti Bhavya; Sparjan Samuvel, Rajan Marystella; Nivetha, Selvaraju; Bhuvaneshwari, Vuyyala; Muralidharan, Kathirvel; Swain, Debasish; Ramalingam, Vaikundamoorthy

doi:10.1039/d3nj04548j

Cited by 3 publications

(2 citation statements)

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“…Initially, DU145 cells (3 × 10 5 /well) were cultivated in a six-well plate for 24 h, and then they were treated with FCN and cocrystals at an IC 50 concentration for another 24 h. Following the treatment, the cells were treated with 10 mg/mL of acridine orange and 5 mg/mL of ethidium bromide before being incubated in the dark for 20 min. To ascertain the apoptotic effect, the cells were examined under a fluorescent microscope after the excess stain was cleaned off …”

Section: Materials and Methodsmentioning

confidence: 99%

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Repurposing of Antifungal Drug Flucytosine/Flucytosine Cocrystals for Anticancer Activity against Prostate Cancer Targeting Apoptosis and Inflammatory Signaling Pathways

Kanagavel,

Sparjan Samuvel,

Ramalingam

et al. 2024

Mol. Pharmaceutics

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This study aimed to repurpose the antifungal drug flucytosine (FCN) for anticancer activity together with cocrystals of nutraceutical coformers sinapic acid (SNP) and syringic acid (SYA). The cocrystal screening experiments with SNP resulted in three cocrystal hydrate forms in which two are polymorphs, namely, FCN-SNP F−I and FCN-SNP F−II, and the third one with different stoichiometry in the asymmetric unit (1:2:1 ratio of FCN:SNP:H 2 O, FCN-SNP F−III). Cocrystallization with SYA resulted in two hydrated cocrystal polymorphs, namely, FCN-SYA F−I and FCN-SYA F−II. All the cocrystal polymorphs were obtained concomitantly during the slow evaporation method, and one of the polymorphs of each system was produced in bulk by the slurry method. The interaction energy and lattice energies of all cocrystal polymorphs were established using solid-state DFT calculations, and the outcomes correlated with the experimental results. Further, the in vitro cytotoxic activity of the cocrystals was determined against DU145 prostate cancer and the results showed that the FCN-based cocrystals (FCN-SNP F−III and FCN-SYA F−I) have excellent growth inhibitory activity at lower concentrations compared with parent FCN molecules. The prepared cocrystals induce apoptosis by generating oxidative stress and causing nuclear damage in prostate cancer cells. The Western blot analysis also depicted that the cocrystals downregulate the inflammatory markers such as NLRP3 and caspase-1 and upregulate the intrinsic apoptosis signaling pathway marker proteins, such as Bax, p53, and caspase-3. These findings suggest that the antifungal drug FCN can be repurposed for anticancer activity.

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Section: Materials and Methodsmentioning

confidence: 99%

“…To ascertain the apoptotic effect, the cells were examined under a fluorescent microscope after the excess stain was cleaned off. 31 2.8.6. Propidium Iodide/DAPI Dual Staining Assay.…”

Section: Hot-stage Microscopymentioning

confidence: 99%