LC-MS and NMR Based Plant Metabolomics: A Comprehensive Phytochemical Investigation of Symphytum anatolicum

Kılınc, Hilal; D’Urso, Gilda; Paolillo, Annunziata; Alankus, Ozgen; Piacente, Sonia; Masullo, Milena

doi:10.3390/metabo13101051

Cited by 5 publications

(2 citation statements)

References 38 publications

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“…One of the common reference standards used in NMR spectroscopy for quantitative analysis is trimethylsilyl propionate (TSP). So, utilizing a known concentration of TSP as a reference, and employing the sophisticated targeted profiling technology provided by the Chenomx software (Chenomx NMR Suite version 10.0), the accurate deconvolution and quantification of identified metabolites was performed [45,46].…”

Section: H-nmr Analysis Of P Granatum Juice and Extractsmentioning

confidence: 99%

Development of a New Extraction Method for Pomegranate and Metabolite Profiling by a LC-MS and 1H NMR Combined Approach

Polcaro,

Samani,

Piacente

et al. 2024

Foods

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The pomegranate (Punica granatum L.) market has steadily grown due to the increasing demand of health-conscious consumers of products with health-promoting effects. Recently, a growing interest in developing ecological and environmentally friendly extraction methods has led to investigating extraction procedures to obtain a higher extraction yield using a lower amount of solvents and energy. Herein, a new extraction procedure was developed to obtain an enriched pomegranate food supplement by using the peels of pomegranate, cultivar “Dente di Cavallo” and its juice. The extraction was performed through a non-conventional extraction technique like SLDE-Naviglio using ethanol and pomegranate juice as a solvent, and peels as a matrix. The extract was analysed by a combined approach based on LCESI/QExactive/MS/MS and NMR analysis, and its chemical profile was compared with those of pomegranate juice and of the extract obtained from peels by SLDE-Naviglio by using ethanol:H2O. The LC-MS analysis highlighted the presence of hydrolysable tannins, flavonoids, ellagic acid and phenol glucoside derivatives, while 1H NMR analysis completed the profile by detecting the primary metabolites. The LC-MS and 1H NMR analysis indicated that the extract obtained by SLDE-Naviglio using ethanol and pomegranate juice was enriched in the bioactives as confirmed by the highest phenolic, tannin and flavonoid content.

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Section: H-nmr Analysis Of P Granatum Juice and Extractsmentioning

confidence: 99%

Development of a New Extraction Method for Pomegranate and Metabolite Profiling by a LC-MS and 1H NMR Combined Approach

Polcaro,

Samani,

Piacente

et al. 2024

Foods

Self Cite

View full text Add to dashboard Cite

show abstract

“…There are a number of software choices for multivariate data analysis and data processing, including MestReNova [ 190 ], MetaboLab [ 191 ], the Soft independent modeling by class analogy (SIMCA) [ 192 ], Metaboanalyst [ 193 ], and Chenomx [ 194 ]. Software exclusively designed for mass spectrometry include XCMS (one of the most popular software for MS) [ 195 ], MZmine [ 196 ], Automated Data Analysis Pipeline (ADAP) [ 196 ], Mass Spectrometry-Data Independent Analysis software (MS-DIAL) [ 197 ] and METLIN [ 198 ].…”

Section: Steps For the Discovery Of Antiviral Compounds In Plant Meta...mentioning

confidence: 99%

Medicinal Plants against Viral Infections: A Review of Metabolomics Evidence for the Antiviral Properties and Potentials in Plant Sources

Adeosun,

Loots

2024

Viruses

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Most plants have developed unique mechanisms to cope with harsh environmental conditions to compensate for their lack of mobility. A key part of their coping mechanisms is the synthesis of secondary metabolites. In addition to their role in plants’ defense against pathogens, they also possess therapeutic properties against diseases, and their use by humans predates written history. Viruses are a unique class of submicroscopic agents, incapable of independent existence outside a living host. Pathogenic viruses continue to pose a significant threat to global health, leading to innumerable fatalities on a yearly basis. The use of medicinal plants as a natural source of antiviral agents has been widely reported in literature in the past decades. Metabolomics is a powerful research tool for the identification of plant metabolites with antiviral potentials. It can be used to isolate compounds with antiviral capacities in plants and study the biosynthetic pathways involved in viral disease progression. This review discusses the use of medicinal plants as antiviral agents, with a special focus on the metabolomics evidence supporting their efficacy. Suggestions are made for the optimization of various metabolomics methods of characterizing the bioactive compounds in plants and subsequently understanding the mechanisms of their operation.

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Symphytum genus—from traditional medicine to modern uses: an update on phytochemistry, pharmacological activity, and safety

Trifan,

Wolfram,

Skalicka-Woźniak

et al. 2024

Phytochem Rev

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With around 34 recognized species, Symphytum genus (comfrey) has a noteworthy position within the Boraginaceae family. Comfrey species have been empirically used since ancient times as wound-healing and skin-regenerating agents in ulcers, wounds, bone fractures, and rheumatic complaints. This review aims to provide a thorough examination of recent scientific advances and challenges within the Symphytum genus, covering data published between 2013 and 2023. It delivers an updated overview of the taxonomy, ethnopharmacological uses, chemical composition, and pharmacological activities of the genus. Special emphasis is put on molecular identification methods for species taxonomy, emerging extraction technologies for comfrey phytochemicals, metabolomics techniques for mapping chemical complexity, modern bioassay platforms revealing its poly-pharmacology, formulation strategies, and remediation approaches for removal of toxic pyrrolizidine alkaloids (PAs). For instance, recent metabolomic studies employing advanced spectro-chromatographic techniques have revealed a diverse chemical composition of comfrey plants, including polysaccharides, allantoin, benzoic and cinnamic acid derivatives, flavonoids, fatty acids, and unsaturated necine-structure-based PAs. The mechanisms underlying their anti-inflammatory, analgesic, wound-healing, anti-irritant, and osteo-regenerative properties were targeted in modern pharmacological setups. Thus, key compounds like allantoin, rosmarinic acid, globoidnans A and B, rabdosiin, and comfreyn A, have been identified as significant contributors to the anti-inflammatory and wound-healing effects of Symphytum-derived preparations. Despite their well-established clinical use, concerns about PAs-induced toxicity have prompted the development of novel PA remediation strategies, enabling the production of comfrey extracts with enhanced safety profiles that can meet the regulatory standards imposed by authorities.

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LC-MS and NMR Based Plant Metabolomics: A Comprehensive Phytochemical Investigation of Symphytum anatolicum

Cited by 5 publications

References 38 publications

Development of a New Extraction Method for Pomegranate and Metabolite Profiling by a LC-MS and 1H NMR Combined Approach

Development of a New Extraction Method for Pomegranate and Metabolite Profiling by a LC-MS and 1H NMR Combined Approach

Medicinal Plants against Viral Infections: A Review of Metabolomics Evidence for the Antiviral Properties and Potentials in Plant Sources

Symphytum genus—from traditional medicine to modern uses: an update on phytochemistry, pharmacological activity, and safety

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